Potassium and Sodium Transport in Yeast

Yenush, Lynne

doi:10.1007/978-3-319-25304-6_8

Cited by 53 publications

(44 citation statements)

References 279 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Potassium is accumulated at high concentrations by all living organisms. For this purpose, cells have developed very efficient transport systems that can work against potassium concentration gradients (see reviews in Ariño, Ramos, & Sychrová, ; Yenush, ). There is an important diversity concerning both the type and the number of plasma membrane potassium transporters in yeast species.…”

Section: The Trk Potassium Uptake Systemmentioning

confidence: 99%

Monovalent cation transporters at the plasma membrane in yeasts

Ariño

Ramos

Sychrová

2018

Yeast

View full text Add to dashboard Cite

Maintenance of proper intracellular concentrations of monovalent cations, mainly sodium and potassium, is a requirement for survival of any cell. In the budding yeast Saccharomyces cerevisiae, monovalent cation homeostasis is determined by the active extrusion of protons through the Pma1 H -ATPase (reviewed in another chapter of this issue), the influx and efflux of these cations through the plasma membrane transporters (reviewed in this chapter), and the sequestration of toxic cations into the vacuoles. Here, we will describe the structure, function, and regulation of the plasma membrane transporters Trk1, Trk2, Tok1, Nha1, and Ena1, which play a key role in maintaining physiological intracellular concentrations of Na , K , and H , both under normal growth conditions and in response to stress.

show abstract

Section: The Trk Potassium Uptake Systemmentioning

confidence: 99%

Monovalent cation transporters at the plasma membrane in yeasts

Ariño

Ramos

Sychrová

2018

Yeast

View full text Add to dashboard Cite

show abstract

“…In the yeast Saccharomyces cerevisiae , large amounts of genomic and functional information have been acquired with respect to key genes involved in salt tolerance including several Na + and K + transporters (Haro et al, ; Posas et al, ; Yenush, ). However, until now, not much has been elucidated about the homologous ion transporters that operate in those fungi to establish symbiotic relationships, whether pathogenic or mutualistic, with plants.…”

Section: Introductionmentioning

confidence: 99%

The endophyte Serendipita indica reduces the sodium content of Arabidopsis plants exposed to salt stress: fungal ENA ATPases are expressed and regulated at high pH and during plant co‐cultivation in salinity

Lanza

Haro

Conchillo

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

Summary Serendipita indica (formerly Piriformospora indica) is an endophytic fungus that colonizes plant roots producing a beneficial effect on plant growth and development under optimal and suboptimal conditions provoked by any biotic or abiotic stress, such as salt stress. Salinity induces osmotic and ionic imbalances in plants, mainly by altering the Na+ and K+ contents. However, the mechanism by which Serendipita improves plant growth has yet to be elucidated. Previous works suggest that this fungus improves the plant osmotic state but not much is known about whether it participates in readjustment of the ionic imbalance in plants. Here, we report that co‐cultivation with Serendipita reduces the Na+ content of Arabidopsis plants under saline conditions. Additionally, we describe the functional characterization of the two Serendipita ENA ATPases, which are homologous to the main proteins involved in the salt tolerance of other fungi. Their heterologous expression in salt‐sensitive yeast mutants shows that SiENA1 is involved in Na+ and K+ efflux, while SiENA5 seems to only be involved in Na+ detoxification. Both are induced and might have a relevant function at alkaline pH, condition in which there are few chlamydospores in the mycelium, as well as during co‐cultivation with plants exposed to saline conditions.

show abstract

“…Therefore, based on previous knowledge we devised a strategy to directly test the presence of Ena1 endocytosis abnormalities in ΔΔ cells. It is known that under salt stress conditions Ena1 expression is induced and the protein is translocated to the plasma membrane to promote sodium efflux (41)(42)(43). Further, Ena1 has been proposed to undergo internalization (44) and vacuolar degradation (45).…”

Section: Resultsmentioning

confidence: 99%

Ubiquitination and Phosphorylation are Independently Required for Epsin-Mediated Internalization of Cargo inS. cerevisiae

A¹,

Hsieh

et al. 2020

Preprint

View full text Add to dashboard Cite

# Equally contributing authorsRunning Title: Lys-Ubiquitination and a Ser/Thr-phosphorylation are required for internalization of a yeast epsin-specific cargo.Endocytosis is a vital cellular process required for multiple cellular functions including nutrient uptake and control of cell membrane composition. This process involves the selective retention of cargo proteins into nascent endocytic sites by the endocytic machinery through the recognition of sequence-encoded motifs or post-translationally added tags.In addition to its classical role in proteosome-mediated protein degradation, ubiquitin (Ub) plays an important role as post-translational tag for protein sorting into different subcellular compartments. Indeed, the Ub-tag mediates membrane protein internalization and targeting to the lysosomal/vacuolar and recycling compartments (reviewed in (1, 2)).Since Ub constitutes the main trafficking signal in Saccharomyces cerevisiae (reviewed in (3)), studies in yeast have been instrumental in advancing our understanding of the mechanisms operating in Ub-mediated sorting. For example, it has been clearly established that budding yeast utilizes Rsp5, the only yeast HECT domain-containing, E3 ubiquitin ligase, to promote cargo ubiquitination relevant to endocytosis and other vesicle trafficking events (3, 4). Indeed, Rsp5-mediated ubiquitination is required for the internalization of cargoes such as the pheromone receptor Ste2 (5, 6), the uracil transporter Fur4 (7), the general amino acid permease Gap1 (8) and the zinc transporter Zrt1 (9) among others.Although Rsp5 is capable of directly interacting with PPxY motif-containing substrates via its WW domains (10-13), this consensus sequence is not present in all cargoes that undergo 14). In those cases, the PPxY motif-containing, Arrestin-Related Trafficking (ARTs) adaptor family specifically bridges cargo proteins to Following ubiquitination, plasma membrane cargoes are recognized by endocytic adaptors, such as epsin and Eps15 (or Ede1 in S. cerevisiae), which contain ubiquitinbinding elements like the Ub-interacting motifs (UIMs) or Ub-Associated (UBA) domains. As a consequence of these interactions, cargo is incorporated into nascent endocytic sites and trafficked towards degradative vacuolar/lysosomal compartments or recycling routes.Specifically, eps15 is known to be responsible for the internalization of the AMPA receptor (21) and Cx43 (22) among other ubiquitinated cargoes; while epsin it is involved in the uptake of vascular endothelial growth factor receptor 2 (VEGFR2) (23), notch ligands (24-27), and the epithelial sodium channel ENaC (28,29).

show abstract

Potassium and Sodium Transport in Yeast

Cited by 53 publications

References 279 publications

Monovalent cation transporters at the plasma membrane in yeasts

Monovalent cation transporters at the plasma membrane in yeasts

The endophyte Serendipita indica reduces the sodium content of Arabidopsis plants exposed to salt stress: fungal ENA ATPases are expressed and regulated at high pH and during plant co‐cultivation in salinity

Ubiquitination and Phosphorylation are Independently Required for Epsin-Mediated Internalization of Cargo inS. cerevisiae

Contact Info

Product

Resources

About