Posttranslational changes in band 3 in adult and aging brain following treatment with ergoloid mesylates, comparison to changes observed in Alzheimer's disease

Cover, Cathleen; Poulin, Jeff E.; Gustafson, Monica; Wyant, Timothy; Gamble, Debra N.; Kay, Marguerite M. B.

doi:10.1016/s0024-3205(96)80004-x

Cited by 5 publications

(9 citation statements)

References 17 publications

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“…Those proteins with the longest half-life will accumulate posttranslational modifications and serve as the best markers for alterations. The normal lifespan of band 3 correlates with the lifespan of the cell (6,19,20,22,24,25). The breakdown of band 3 and the accumulation of SCA on cells triggers removal of that cell (6,8,9,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 97%

“…To date, our aging studies indicate that the anion transport ability of band 3 decreases in brains and erythrocytes from old mice (10,19,(23)(24)(25)(26)(27). This decreased transport ability precedes obvious structural changes such as band 3 degradation and generation of SCA (10,19,23,(20)(21)(22)(23)(24)(25)(26)(27) and occurs concommitantly with generation of aged/altered band 3.…”

Section: Discussionmentioning

confidence: 99%

“…E and 200 mg/kg of 13- (9,19). Splenic lymphocytes were isolated by Ficoll/Hypaque density separation as described (19).…”

mentioning

confidence: 99%

“…After lymphocyte isolation, cells were extensively washed using influx buffer (300 mM sucrose/30 mM Tris-Hepes, pH 7.0) and anion transport was performed as described (9,15,16,19) except that 0.25 mCi (1 Ci = 37GBq) of radioactive sulfate (Na235SO4 from ICN) was added to each cell suspension (final concentration, 0.0625 mCi/ml). Cpm were converted to rates (pmol per 2 x 106 cells per min) for analysis.…”

mentioning

confidence: 99%

“…The immunodot assay with antibodies to aged or altered band 3 was performed as described (19 Fig. 1).…”

mentioning

confidence: 99%

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Vitamin E prevents oxidative modification of brain and lymphocyte band 3 proteins during aging.

Poulin

Cover

Gustafson

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Antioxidants may play an important role in preventing free radical damage associated with aging by interfering directly in the generation of radicals or by scavenging them. We investigated the effects of a high vitamin E and/or a high 8-carotene diet on aging of the anion transporter, band 3, in lymphocytes and brain. The band 3 proteins function as anion transporters, acid base regulators, CO2 transporters, and structural proteins that provide a framework for membrane lipids and that link the plasma membrane to the cytoskeleton. Senescent cell antigen (SCA), which terminates the life of cells, is a degradation product of band 3. This study was conducted as a double-blind study in which eight groups of middle-aged or old mice received either high levels of a-carotene and/or vitamin E or standard levels of these supplements in their diets. Anion transport kinetic assays were performed on isolated splenic lymphocytes. Immunoreactivity of an antibody that recognizes aging changes in old band 3 preceding generation of SCA was used to quantitate aged band 3 in brain tissue. Results indicate that vitamin E prevented the observed age-related decline in anion transport by lymphocytes and the generation of aged band 3 leading to SCA formation. 18-Carotene had no significant effect on the results of either assay. Since increased aged band 3 and decreased anion transport are initial steps in band 3 aging, which culminates in the generation of SCA and cellular removal, vitamin E prevents or delays aging of band 3-related proteins in lymphocytes and brain.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

“…E and 200 mg/kg of 13- (9,19). Splenic lymphocytes were isolated by Ficoll/Hypaque density separation as described (19).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The immunodot assay with antibodies to aged or altered band 3 was performed as described (19 Fig. 1).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Vitamin E prevents oxidative modification of brain and lymphocyte band 3 proteins during aging.

Poulin

Cover

Gustafson

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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HeTDR: Drug repositioning based on heterogeneous networks and text mining

Jin

Niu²,

Jiang

et al. 2021

Patterns

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Circulating Autoantibodies Recognize and Bind Dying Neurons Following Injury to the Brain

Stein¹,

Fedynyshyn²,

Kalil³

2002

J Neuropathol Exp Neurol

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While it is known that autoimmune cells can protect against cell damage following traumatic injury of the brain, the role of autoantibodies in brain injury is less clear. Here we present evidence in adult rats that following a cortical lesion of the brain, circulating IgG autoantibodies bind to dying neurons in the vicinity of the lesion. At intervals that ranged from 4 h to 7 days after making a unilateral lesion of visual cortex, we observed neurons near the lesion that were immunopositive for rat IgG. Many of these IgG-positive neurons were in advanced stages of degeneration. The magnitude of the immunostaining observed was directly proportional to the percent reactivity to rat IgG of the antibodies that were used. Preadsorption of the antibodies with rat serum eliminated the immunostaining. In addition, immunostaining for serum albumin in sections through the cortical lesion was negative, supporting the conclusion that the positive staining for IgG does not result from the passive diffusion of serum proteins into injured cells. Instead, the evidence presented here strongly suggests that naturally occurring IgG autoantibodies bind specifically to dying neurons in the injured brain. We propose that this autoantibody binding may participate in the phagocytosis and removal of injured neurons.

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Posttranslational changes in band 3 in adult and aging brain following treatment with ergoloid mesylates, comparison to changes observed in Alzheimer's disease

Cited by 5 publications

References 17 publications

Vitamin E prevents oxidative modification of brain and lymphocyte band 3 proteins during aging.

Vitamin E prevents oxidative modification of brain and lymphocyte band 3 proteins during aging.

HeTDR: Drug repositioning based on heterogeneous networks and text mining

Circulating Autoantibodies Recognize and Bind Dying Neurons Following Injury to the Brain

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