Postprandial Dried Blood Spot–Based Nutritional Metabolomic Analysis Discriminates a High-Fat, High-Protein Meat-Based Diet from a High Carbohydrate Vegan Diet: A Randomized Controlled Crossover Trial

McNairn, Megan; Brito, Alex; Dillard, Kayla; Heath, Hannah; Pantaleon, Matthew; Fanter, Rob; Pilolla, Kari; Amin, Samir; Frano, Michael R. La

doi:10.1016/j.jand.2020.10.024

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…This study demonstrates that dietary metabolomics in DBS was able to distinguish the HFPM and HCV diets and can be an effective approach to monitoring food intake. It may be a useful tool to provide a more objective measure of food intake and enable a complementary alternative to conventional dietary assessment procedures [ 84 ].…”

Section: Microsampling Applications Monitoring Lipids and Metabolitesmentioning

confidence: 99%

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids

Bossi,

Limo,

Pagani

et al. 2024

Metabolites

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Blood serves as the primary global biological matrix for health surveillance, disease diagnosis, and response to drug treatment, holding significant promise for personalized medicine. The diverse array of lipids and metabolites in the blood provides a snapshot of both physiological and pathological processes, with many routinely monitored during conventional wellness checks. The conventional method involves intravenous blood collection, extracting a few milliliters via venipuncture, a technique limited to clinical settings due to its dependence on trained personnel. Microsampling methods have evolved to be less invasive (collecting ≤150 µL of capillary blood), user-friendly (enabling self-collection), and suitable for remote collection in longitudinal studies. Dried blood spot (DBS), a pioneering microsampling technique, dominates clinical and research domains. Recent advancements in device technology address critical limitations of classical DBS, specifically variations in hematocrit and volume. This review presents a comprehensive overview of state-of-the-art microsampling devices, emphasizing their applications and potential for monitoring metabolites and lipids in blood. The scope extends to diverse areas, encompassing population studies, nutritional investigations, drug discovery, sports medicine, and multi-omics research.

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Section: Microsampling Applications Monitoring Lipids and Metabolitesmentioning

confidence: 99%

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids

Bossi,

Limo,

Pagani

et al. 2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…Metabolomics is a promising approach to objectively assess the impact of diet on chronic disease risk by quantifying small molecules in a biological system [1,15]. The majority of published literature using untargeted metabolomics in nutrition research has been biomarker discovery based on individual foods and dietary patterns [15][16][17][18][19][20]. Fewer studies have compared metabolomic profiles among dietary fats and oils [21], especially compared metabolite profiling between soybean oil and partially-hydrogenated fat [22,23] or determined the association with underlying metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Plasma Metabolome Response to Diets Enriched in Soybean and Partially-Hydrogenated Soybean Oil in Moderately Hypercholesterolemic Adults-A Pilot Study

et al. 2023

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Partially-hydrogenated fat/trans fatty acid intake has been associated with adverse effects on cardiometabolic risk factors. Comparatively unexplored is the effect of unmodified oil relative to partially-hydrogenated fat on the plasma metabolite profile and lipid-related pathways. To address this gap, we conducted secondary analyses using a subset of samples randomly selected from a controlled dietary intervention trial involving moderately hypercholesterolemic individuals. Participants (N = 10, 63 ± 8 y, BMI, 26.2 ± 4.2 kg/m2, LDL-C, 3.9 ± 0.5 mmol/L) were provided with diets enriched in soybean oil (SO) and partially-hydrogenated soybean oil (PHSO). Plasma metabolite concentrations were determined using an untargeted approach and pathway analysis using LIPIDMAPS. Data were assessed using a volcano plot, receiver operating characteristics curve, partial least square-discrimination analysis and Pearson correlations. Among the known metabolites higher in plasma after the PHSO diet than the SO diet, the majority were phospholipids (53%) and di- and triglycerides (DG/TG, 34%). Pathway analysis indicated upregulation of phosphatidylcholine synthesis from DG and phosphatidylethanolamine. We identified seven metabolites (TG_56:9, TG_54:8, TG_54:7, TG_54:6, TG_48:5, DG_36:5 and benproperine) as potential biomarkers for PHSO intake. These data indicate that TG-related metabolites were the most affected lipid species, and glycerophospholipid biosynthesis was the most active pathway in response to PHSO compared to SO intake.

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Dietary Assessment and Metabolomic Methodologies in Human Feeding Studies: A Scoping Review

Clarke,

Ferguson,

Stanford

et al. 2023

Advances in Nutrition

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Postprandial Dried Blood Spot–Based Nutritional Metabolomic Analysis Discriminates a High-Fat, High-Protein Meat-Based Diet from a High Carbohydrate Vegan Diet: A Randomized Controlled Crossover Trial

Cited by 5 publications

References 29 publications

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids

Comparison of Plasma Metabolome Response to Diets Enriched in Soybean and Partially-Hydrogenated Soybean Oil in Moderately Hypercholesterolemic Adults-A Pilot Study

Dietary Assessment and Metabolomic Methodologies in Human Feeding Studies: A Scoping Review

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