Postpartum human breast milk levels of neutrophil gelatinase-associated lipocalin (NGAL) and matrix metalloproteinase-9 (MMP-9)/NGAL complex in normal and pregnancies complicated with insulin-dependent gestational diabetes mellitus. A prospective pilot case-control study

Metallinou, Dimitra; Lykeridou, Katerina; Karampas, Grigorios; Liosis, Georgios; Skevaki, Chrysanthi; Rizou, Myrto; Papassotiriou, Ioannis; Rizos, Demetrios

doi:10.1080/01443615.2019.1628191

Cited by 6 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metallinou et al evaluated the levels of Neutrophil gelatinase-associated lipocalin (NGAL) and its complex with matrix metalloproteinase-9 (MMP-9) in the colostrum and found that the mean complex concentration was significantly higher in diabetic pregnancies in comparison to normal ones [26].…”

Section: Anti-infective Proteinsmentioning

confidence: 99%

Influence of Diabetes during Pregnancy on Human Milk Composition

Peila

Gazzolo²,

Bertino

et al. 2020

Nutrients

View full text Add to dashboard Cite

Human milk (HM) is a unique nourishment believed to contain biological factors contributing to both short and long-term benefits. Considering that a mother’s own milk is often considered the first choice for nutrition of neonates, an aspect of increased interest is the possible effect of diabetes on the mammary gland and therefore on breast milk composition. This article aims to review the published literature on this topic, and to offer additional insights on the role of this disease on the composition of HM. This review was performed by searching the MEDLINE, EMBASE, CINHAL and Cochrane Library databases. A total of 50 articles were selected, focused specifically on one of the two types of diabetes: gestational diabetes mellitus (21 studies) and insulin-dependent diabetes mellitus (8 studies). Overall, the findings from the literature suggest that diabetes can alter the composition of HM. Nevertheless, the studies in this field are scarce, and the related protocols present some limitations, e.g., evaluating the variability of just a few specific milk biochemical markers in association with this syndrome.

show abstract

Section: Anti-infective Proteinsmentioning

confidence: 99%

Influence of Diabetes during Pregnancy on Human Milk Composition

Peila

Gazzolo²,

Bertino

et al. 2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…Despite significant medical progress in recent years, cancer is still one of the most common causes of death. Early-stage tubular adenocarcinoma of the colon is usually cured by surgical excision, but it is difficult to detect this disease in the early stage[ 10 , 11 ], which is due to the lack of effective biomarkers. More importantly, it is necessary to determine the progression of tubular adenocarcinoma of the colon during treatment, to allow efficient evaluation and adjustment of therapeutic strategies.…”

Section: Discussionmentioning

confidence: 99%

“…NGAL and MMP-9 have been reported to be involved in several types of cancers and have been used as biomarkers to indicate disease progression[ 10 - 15 ]. In our study, we found that the expression of NGAL was positively related to the occurrence of tubular adenocarcinoma of the colon using qRT-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Advances in the understanding of colorectal carcinogenesis offer opportunities to identify biomarkers for earlier diagnosis. Recent studies demonstrated that neutrophil gelatinase-associated lipocalin (NGAL) and matrix metalloproteinase-9 (MMP-9) could be used as diagnostic and prognostic biomarkers in various cancers, including breast cancer[ 10 ], bladder cancer[ 11 , 12 ], gastric cancer[ 13 ], endometrial cancer[ 14 ] and kidney tumors[ 15 ]. NGAL, also called lipocalin-2 (LCN2), is a small glycoprotein, which is encoded by the LCN2 gene.…”

Section: Introductionmentioning

confidence: 99%

“…The increased expression level of MMP-9 has been observed in several cancers, which was related to the aggressiveness of cancer cells and the overall survival of patients[ 23 ]. In combination with NGAL, MMP-9 can avoid proteolytic degradation, which increases its enzymatic activity and the movability of malignancies[ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Combination of neutrophil gelatinase-associated lipocalin and matrix metalloproteinase-9 are biomarkers for the detection of colon tubular adenocarcinoma

Yuan¹,

Xie²,

Zhu³

et al. 2021

WJGO

View full text Add to dashboard Cite

BACKGROUND Tubular adenocarcinoma of the colon, which originates from the epithelium of the glands, is a major health concern worldwide. However, it is difficult to detect at an early stage. The lack of biomarkers is a main barrier to the diagnosis and treatment of tubular adenocarcinoma. Neutrophil gelatinase-associated lipocalin (NGAL) is a secreted protein that induces the expression of matrix metalloproteinase-9 (MMP-9) and is involved in various tumors. NGAL and MMP-9 have been reported to be associated with tumorigenesis and development. They may have potential as biomarkers for diagnosis of tubular adenocarcinoma of the colon. AIM To determine whether NGAL and MMP-9 can be used as potential biomarkers to indicate the progression of tubular adenocarcinoma of the colon. METHODS Samples were collected from surgically excised tissue from various patients. The content of pro-gastrin-releasing peptide (pro-GRP) in the serum was measured by an electrochemiluminescence immunoassay. The expression patterns of NGAL and MMP-9 and the relationship between NGAL and MMP-9 were examined by quantitative real-time PCR, Western blotting and immunohistochemical analysis. RESULTS In this study, we found that NGAL and MMP-9 can be used as biomarkers for the detection of tubular adenocarcinoma of the colon and that their combination improved diagnostic accuracy. By analyzing the expression of NGAL in tubular adenocarcinoma at different levels, we found that NGAL expression was significantly upregulated in primary tubular adenocarcinoma tissues compared with normal tissues. The upregulation of NGAL expression was strongly correlated with both the degree of differentiation and the disease stage (I–III), indicating that NGAL could serve as a diagnostic biomarker for tubular adenocarcinoma. When using NGAL as a biomarker for diagnosis, the accuracy was similar to that achieved with the widely used biomarker pro-GRP, suggesting that NGAL is reliable. Moreover, the expression of MMP-9 was also strongly correlated with the differentiation stage, demonstrating that MMP-9 could be used as a biomarker to indicate the progression of tubular adenocarcinoma of the colon. More importantly, the combination of NGAL and MMP-9 produced a more accurate diagnosis of tubular adenocarcinoma, and these results were further confirmed by immunohistochemical analysis of tissue sections. CONCLUSION Our study demonstrated that both NGAL and MMP-9 can be used as biomarkers for the diagnosis of colon tubular adenocarcinoma and that the results could be further improved by combining them.

show abstract