Postovulatory aging causes the deterioration of porcine oocytes
            <i>via</i>
            induction of oxidative stress

Miao, Yun; Zhou, Changyin; Cui, Zhaokang; Zhang, Mianqun; ShiYang, Xiayan; Lu, Yajuan; Xiong, Bo

doi:10.1096/fj.201700908r

Cited by 55 publications

(53 citation statements)

References 33 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Excessive production of reactive oxygen species has detrimental effects on cellular metabolism and other physiological activities. Studies have found that excessive levels of ROS can cause deterioration in oocytes quality and disrupts the meiotic process (Chaube et al ; Miao et al ). After exposure to IBP, we assessed the level of oxidative stress in the cytoplasm of oocytes.…”

Section: Discussionmentioning

confidence: 99%

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Meng

Jiao

Chen

et al. 2020

Environ and Mol Mutagen

View full text Add to dashboard Cite

As a member of parabens (PBs), Isobutylparaben (IBP) has a broad-spectrum antimicrobial activity and widely used in personal care products and cosmetics. Recent studies have indicated that usage of IBP poses a potential threat to reproductive health. In this study, we aimed to reveal the effects of acute exposure to IBP on the meiotic maturation of porcine cumulus oocyte complexes. Initial study showed that 200 μM of IBP significantly reduced the rate of the first polar body extrusion with no significant effect on cumulus cell expansion; however, 400 μM of IBP could significantly affect both. Further research revealed that abnormal spindles, misalignment chromosomes, and aberrant distributed actin filaments were detected in IBP-treated oocytes, which indicates that the cytoskeleton architecture of oocyte could be the target of IBP. At the same time, ROS level and apoptosis rate of oocyte were significantly increased by IBP exposure. Moreover, the levels of H3K9me3 and H3K27me3 were significantly induced in oocytes by IBP. Collectively, these results demonstrate that acute exposure to IBP could disrupt porcine oocyte maturation through affecting cytoskeleton, oxidative stress, viability and epigenetic modification. Environ. Mol. Mutagen. 61:433-444, 2020. Fig. 7. Schematic representation of negative effects of IBP on porcine oocytes. Oocyte exposure to IBP during meiotic maturation leads to inhibition of cumulus cell expansion, disruption of cytoskeleton, generation of oxidative stress and apoptosis, and alteration of epigenetic modifications. Environmental and Molecular Mutagenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Meng

Jiao

Chen

et al. 2020

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Very little information is available about antiaging effectors in oocytes, and the mechanism for rescuing or preventing oocyte aging Several studies have investigated the degradation of oocyte quality with aging time. Embryos from oocytes aged for more than 48 hr rarely develop into blastocysts (Lee et al, 2014;Miao et al, 2018;Němeček, Dvořáková, Heroutová, Chmelíková, & Sedmíková, 2017). Although aging for 24 hr also decreases oocyte competence, this effect can be recovered by antiaging systems (Lee et al, 2014;Ma, Zhang, Zhang, Han, & Rui, 2015).…”

Section: Discussionmentioning

confidence: 99%

Allicin protects porcine oocytes against damage during aging in vitro

Park

Lee

Yoon

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

Allicin, a chemical component of garlic, has strong antioxidant activity and is thought to exert antiaging effects in vitro. We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. The rates of oocyte survival and polar body extrusion in samples treated with 1 µM allicin (1 AL) were significantly higher than in untreated samples (0 AL). In addition, 1 AL prevented defects in spindle formation and chromosome alignment, as well as decreases in the expression of maturation markers, during in vitro aging. In this study, we considered allicin to be a regulator of autophagy rather than an antioxidant or antiapoptotic agent. At the embryo level, although the cleavage rate after parthenogenetic activation was similar in all groups, the blastocyst formation rate was higher in the 1 AL group than in the 0 AL group. Our findings demonstrate that allicin effectively prevents the deterioration of porcine oocytes during aging in vitro, and could therefore be used to improve the quality of aged oocytes used in in vitro experiments.

show abstract

“…With the treatment of increasing doses of the inhibitor, the oocytes exhibited a significantly decreased rate of polar body extrusion with poor expansion of cumulus cells, suggesting that inhibition of Eg5 perturbs the meiotic progression. Previous studies by us and others have shown that oocyte meiotic arrest is largely caused by the defective spindle assembly and chromosome alignment [ 31 , 36 , 37 ]. Additionally, Eg5 has been found to sort and push microtubules apart to drive the separation of spindle poles [ 12 ], and loss of Eg5 leads to cell cycle arrest and defective centrosome separation resulting in the development of monopolar spindles [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundKinesin superfamily proteins are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. However, their exact roles during mammalian oocyte meiosis have not been fully clarified.ResultsHerein, we investigated the critical events during porcine oocyte meiotic maturation with the treatment of Eg5-specific inhibitor monastrol. We found that Eg5 inhibition resulted in oocyte meiotic failure by displaying the poor expansion of cumulus cells and reduced rate of polar body extrusion. In the meantime, the spindle assembly and chromosome alignment were compromised, accompanied by the decreased level of acetylated α-tubulin, indicative of less stable microtubules. Impaired actin dynamics and mitochondria integrity were also observed in Eg5-inhibited oocytes. Additionally, inhibition of Eg5 caused the abnormal distribution of cortical granules and ovastacin, a cortical granule component, potentially leading to the fertilization failure.ConclusionsOur findings reveal that Eg5 possesses an important function in porcine oocyte meiotic progression by regulating the organelle dynamics and arrangement.

show abstract

Postovulatory aging causes the deterioration of porcine oocytes via induction of oxidative stress

Cited by 55 publications

References 33 publications

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Allicin protects porcine oocytes against damage during aging in vitro

Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement

Contact Info

Product

Resources

About