Postoperative Infection With Pseudomonas Aeruginosa in an Eye Hospital

Ayliffe, G.A.J.; Barry, D. R.; Lowbury, E. J. L.; Roper-Hall, M. J.; Walker, Woolf

doi:10.1016/s0140-6736(66)91135-4

Cited by 59 publications

(6 citation statements)

References 12 publications

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“…Conflicting views have been presented regarding the frequency and importance of microbial contamination of eye drops. Thus bacterial growth from eye drop containers and the occurrence of serious ocular infections following the use of contaminated solutions have been reported by among others Allen & Mangiaracine (1964), Ayliffe et al (1966), Harte et al (1978), McCulloch (1943), Schmelzer Hervding Contamination of in-use eye drops (1965) and Vaughn (1955). On the other hand, some studies show only a negligible incidence of bacterial growth (Barkman et al 1969;Hugo & Wilson 1970;h u n d et al 1978).…”

mentioning

confidence: 90%

Bacterial Contamination of Drops and Dropper Tips of In‐use Multidose Eye Drop Bottles

Høvding

Sjursen

1982

Acta Ophthalmologica

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The type and frequency of bacterial contamination of 638 in-use multidose eye drop bottles are reported. By dripping, bacteria were recovered from 82 bottles (12.9%). Bacterial growth considered to be clinically and microbiologically significant were found in 12 cases (1.9%). The swabbings from 38 of 180 dropper tips (2 1.1 %) yielded bacteria, while none of the pipette aspirates from 196 bottles were positive. The flora obtained by dripping and swabbing was very similar to the normal conjunctival and skin flora. It is suggested that microorganisms isolated by dripping often originate from contaminated dropper tips. The frequency of contaminated drops did not increase with increasing duration of use of the bottles. Repeated examinations and inoculation studies indicated that the solutions were self-sterilizing. This indicates that multidose eye drop bottles preserved and dispensed as in the present study may be used for more than the usual 4 weeks without increasing the risk of ocular infection.

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confidence: 90%

Bacterial Contamination of Drops and Dropper Tips of In‐use Multidose Eye Drop Bottles

Høvding

Sjursen

1982

Acta Ophthalmologica

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“…Pseudomonas aeruginosa has been involved in the contamination of ophthalmic phanmaceuticals, and severe ocular infections have occurred as a result of using these medicaments (4, 5, 17). P. aeruginosa is a major contaminant of water used in the preparation of pharmaceutical products (3,13,19). Favero et al (8) have shown that P. aeruginosa is capable of growth in filtersterilized distilled water.…”

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confidence: 99%

Resistance of selected strains of Pseudomonas aeruginosa to low-intensity ultraviolet radiation

Abshire¹,

Dunton²

1981

Appl Environ Microbiol

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The resistances of 10 strains of Pseudomonas aeruginosa and other microorganisms to an ultraviolet (UV) intensity of 100 muW/cm2 were determined. Organisms were exposed in 2- or 15-ml saline suspensions contained in uncapped polyethylene bottles for increasing periods of time, and the surviving fractions were enumerated. Decimal reduction times were calculated by regression analysis, using the least-squares method. The 10 strains of P. aeruginosa, compared with Micrococcus radiodurans and Candida albicans, were very susceptible to low-intensity UV radiation. Results from this study showed that a UV intensity of 100 muW/cm2 penetrated saline suspensions up to 40 mm deep sufficiently to kill high levels of microbial cells, especially P. aeruginosa cells. These results allowed us to design a system for determining and monitoring the sterilization capability of low-intensity UV radiation. In our particular case, UV proved to be an efficient mode for sterilizing saline suspensions of P. aeruginosa in polyethylene bottles. The significance and application of these findings with regard to supporting UV as a sterilant are discussed.

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“…But after intraocular operations, sepsis is nearly always very serious; in almost every case there is a real possibility that the eye will be lost. Thus, in a series of 22 infections (Allen & Mangiaracine 1964), evisceration of the eye was necessary in 11 and useful vision was lost in a further 7; in the Birmingham outbreak 15 infections led to the loss of 6 eyes (Ayliffe et al 1966).…”

Section: Results and Incidence Ofpost-operative Sepsismentioning

confidence: 99%

Control of Infection in Ophthalmic Surgery

Blowers

1967

Proceedings of the Royal Society of Medicine

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Postoperative Infection With Pseudomonas Aeruginosa in an Eye Hospital

Cited by 59 publications

References 12 publications

Bacterial Contamination of Drops and Dropper Tips of In‐use Multidose Eye Drop Bottles

Bacterial Contamination of Drops and Dropper Tips of In‐use Multidose Eye Drop Bottles

Resistance of selected strains of Pseudomonas aeruginosa to low-intensity ultraviolet radiation

Control of Infection in Ophthalmic Surgery

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