Postnatal testicular development in the Chinchilla rabbit

Vigueras-Villaseñor, Rosa María; Montelongo-Solís, Paola; Chávez-Saldaña, Margarita; Gutiérrez-Pérez, Oscar; Arteaga-Silva, Marcela; Rojas-Castañeda, Julio César

doi:10.1016/j.acthis.2013.02.004

Cited by 4 publications

(5 citation statements)

References 30 publications

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“…Taking advantage of the traceability of Venus expression, developmental stage-specific expression of this ectopic protein was examined. The postnatal testicular development of rabbit is well described and although it reveals some rabbit breed specific differences, overall its timing resembles that of human [ 24 – 26 ]. Therefore, we compared the testes of young bucks of the # SB3JT line at the pre-spermatogenesis stage (42dpp), at 60 dpp, the beginning of spermatogenesis and in adult buck’s testis (120 dpp).…”

Section: Resultsmentioning

confidence: 99%

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits

et al. 2016

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The Sleeping Beauty transposon system was established as a robust and efficient method for germline transgenesis in different mammalian species. The generation of transgenic mice, rats, rabbits and swine carrying an identical Venus reporter construct delivered by transposon-mediated gene transfer enables comparative studies of gene expression in these lines of mammalian models. Whereas comparable expression patterns of the Venus reporter were found in somatic tissues, preliminary studies suggested that a striking difference in reporter expression may exist in mature spermatozoa of these species. Here we clearly show the differential expression of Venus reporter protein during spermatogenesis of the two compared species, the laboratory rabbit and mice. We provide evidence for the functionality of intercellular bridges in the male germline and genotype-independent transgenic phenotype of rabbit spermatids. Our data suggest that the reporter rabbit line may be a suitable tool to identify molecular mechanisms in testicular development, and may contribute to develop better animal models for male infertility in men.

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Section: Resultsmentioning

confidence: 99%

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits

et al. 2016

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“…Two groups (n = 12 each, control and COI) of rabbits ( Oryctolagus cuniculus , European Chinchilla breed) were randomly assorted, and COI rabbits were treated as follows: seven days after birth, periodic subcutaneous administration of 17β-estradiol 3-benzoate (E2, acting as an endocrine disruptor (ED), SIGMA, Burlington, MA, USA) was started, inoculating 16.6 µg E2 every third day (as a diluent, corn oil was used, total dose of 500 μg); this pharmacological protocol acts on a temporal window in which testis descending is irreversibly prevented [ 46 , 71 ]. The control group was inoculated with a vehicle solution.…”

Section: Methodsmentioning

confidence: 99%

Downregulation of Serotonergic System Components in an Experimentally Induced Cryptorchidism in Rabbits

Jiménez-Trejo,

Arriaga-Canon,

Herrera

et al. 2024

IJMS

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Cryptorchidism (CO) or undescended testes is defined as the failure of one or both testes to be positioned inside the scrotum. Typically, cryptorchidism is detected at birth or shortly thereafter, and in humans, it is considered to be part of the testicular dysgenesis syndrome (TDS), a complex pathology regarding the male reproductive system that apparently involves the interaction of both genetic and environmental harmful factors, mainly during embryonic development. Serotonin (5-HT) is an ancient molecule that participates in a broad range of body functions, and in recent years, its importance in reproduction has started to be elucidated. In male pathologies such as infertility, varicocele, erectile dysfunction, and primary carcinoid tumors, an increase in 5-HT concentration or its metabolites in the blood, semen, and urine has been directly related; nevertheless, the role of 5-HT in CO remains unknown. In the present work, our goal was to answer two important questions: (1) whether some serotonergic system components are present in adult male Oryctolagus cuniculus (chinchilla rabbit) and (2) if there are changes in their expression in an experimental model of CO. Using histological, molecular, and biochemical approaches, we found the presence of some serotonergic system components in the adult chinchilla rabbit, and we demonstrated that its expression is downregulated after CO was pharmacologically induced. Although we did not test the role of 5-HT in the etiology of CO, our results suggest that this indoleamine could be important for the regulation of steroidogenesis and spermatogenesis processes in the chinchilla rabbit during adulthood. Finally, in parallel experimental series, we found downregulation of kynurenine concentration in COI rabbits when compared to control ones, suggesting that CO could be affecting the kynurenine pathway and probably testicular immune privilege which in turn could lead to infertility/sterility conditions in this disorder.

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“…Recientemente se ha mostrado que el gonocito neonatal migra entre las células de Sertoli hacia la periferia del cordón entre los 3 y 9 meses de edad en humanos, a los 2 y 6 días de edad en ratones y de los 45-50 días en el conejo (Vigueras-Villaseñor et al 2013;Docampo y Hadziselimovic 2015). Se ha establecido que con la separación placentaria, se activa el eje hipotálamo-hipófisis-gónada y los cambios hormonales que se producen se identifican como mini pubertad.…”

Section: Infertilidad Masculinaunclassified

“…Es importante señalar que la EGCG, evidenció estos efectos también en el grupo CS/EGCG, lo que indicó que las alteraciones inespecíficas reportadas para el conejo raza chinchilla (Vigueras et al, 2013) son reducidas al administrar la EGCG. Esto se vio reflejado en una espermatogénesis completa, incrementando la proliferación, la esteriodogénesis y estimulando al sistema antioxidante endógeno en conejos sanos.…”

Section: Egcg Y Lipoperoxidaciónunclassified

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Efecto de la epigalocatequina-3-galato sobre las alteraciones producidas en la cripcoquidia experimental

Cabrera

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Postnatal testicular development in the Chinchilla rabbit

Cited by 4 publications

References 30 publications

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits

Downregulation of Serotonergic System Components in an Experimentally Induced Cryptorchidism in Rabbits

Efecto de la epigalocatequina-3-galato sobre las alteraciones producidas en la cripcoquidia experimental

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