Postnatal testicular development and testosterone changes in Ghezel ram lambs

Nazari-Zenouz, F.; Moghaddam, Gh; Hamidian, Gholamreza; Ashrafi, Javad; Rafat, S.A.; Qasemi-Panahi, Babak

doi:10.1016/j.smallrumres.2016.07.001

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…The plasma testosterone values at D60 when the animals were 6 months old were similar to those previously described for sheep. In the present study, a plasma testosterone value of 1.75 ng/mL was found in the control group, close to that reported by Nazari-Zenouz et al (2016) in 6-month-old lambs (1.69 ng/mL). Gokdal et al (2010), Tesema et al (2019), andNeedham et al (2019) investigated the effects of anti-GnRH immunocastration on the reproductive characteristics of lambs.…”

Section: Plasma Testosterone Concentrationsupporting

confidence: 90%

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

et al. 2021

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The objective of this study was to evaluate the testicular biometric, seminal, and plasma testosterone levels in lambs subjected to an anti-GnRH vaccine as a method of castration. Thirty entire, crossbred Santa Inês male lambs were randomly distributed into three treatment (T): T1 was the control group, with the administration of 1 mL of saline solution subcutaneously (SC); 1.0 and 0.5 mL of an anti-GnRH vaccine were administered SC in T2 and T3, respectively. Testicular biometric variables, physical and morphological variables of semen, and plasma testosterone concentrations were evaluated. At D60, there was a reduction in testicular length, width, thickness, and scrotal circumference of the immunocastrated animals regardless of the vaccine dose used (P < 0.05). A reduction in semen physical variables at both dosages (P < 0.05) was observed, with azoospermia, in 80% and 70% of animals in the T2 and T3 groups, respectively. At D60, the immunocastrated animals also showed an increase in spermatozoa defects (P < 0.05), whereas plasma testosterone concentration decreased (P < 0.05). Immunocastration of lambs using the Bopriva vaccine at doses of 1.0 and 0.5 mL is efficient in inducing azoospermia in up to 80% of animals, although two doses in a 30-day interval are necessary for it to be an effective and safe method. Efficacy was demonstrated through a reduction in serum testosterone levels, testicular biometry, and seminal fluid analysis. Considering the efficacy of both doses in this study, we recommend using the lower dose (0.5 mL), which will allow for a 50% reduction in vaccine costs.

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Section: Plasma Testosterone Concentrationsupporting

confidence: 90%

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

et al. 2021

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“…31 The lumen of the seminiferous tubules and spermatocytes appeared by 4 months of age in Ghezel ram. 32 Our results showed that the seminiferous tubule diameter increased from 0 days, with a slow increase between 0 and 30 days and a rapid increase from 30 days to 180 days. In addition, during the 180-day stage, advanced spermatogenic cells, such as various stages of spermatocytes, round/elongating spermatids and spermatozoa, and the seminiferous tubule lumen were discernible.…”

Section: Discussionsupporting

confidence: 48%

“…The lumen appears at 12 weeks and spermatocytes appear at 15 weeks in black Bailey sheep 31 . The lumen of the seminiferous tubules and spermatocytes appeared by 4 months of age in Ghezel ram 32 . Our results showed that the seminiferous tubule diameter increased from 0 days, with a slow increase between 0 and 30 days and a rapid increase from 30 days to 180 days.…”

Section: Discussionsupporting

confidence: 44%

Study of spermatogenic and Sertoli cells in the Hu sheep testes at different developmental stages

Yang

Zhao

et al. 2023

The FASEB Journal

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Spermatogenesis is a highly organized process by which undifferentiated spermatogonia self‐renew and differentiate into spermatocytes and spermatids. The entire developmental process from spermatogonia to sperm occurs within the seminiferous tubules. Spermatogenesis is supported by the close interaction of germ cells with Sertoli cells. In this study, testicular tissues were collected from Hu sheep at 8 timepoints after birth: 0, 30, 90, 180, 270, 360, 540, and 720 days. Immunofluorescence staining and histological analysis were used to explore the development of male germ cells and Sertoli cells in the Hu sheep testes at these timepoints. The changes in seminiferous tubule diameter and male germ cells in the Hu sheep testes at these different developmental stages were analyzed. Then, specific molecular markers were used to study the proliferation and differentiation of spermatogonia, the timepoint of spermatocyte appearance, and the maturation and proliferation of Sertoli cells in the seminiferous tubules. Finally, the formation of the blood–testes barrier was studied using antibodies against the main components of the blood–testes barrier, β‐catenin, and ZO‐1. These findings not only increased the understanding of the development of the Hu sheep testes, but also laid a solid theoretical foundation for Hu sheep breeding.

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“…Two major changes in testicular development were also observed in sheep and salmon. In a study on Ghezel sheep (an early-puberty native Iranian sheep), the number of spermatogonia in seminiferous tubules increases remarkably from the age of 3 months, and spermatocyte and seminiferous tubule lumen are apparently observed from the age of 4 months [10]. Some low vertebrates have similar phenomena.…”

Section: Developmental Stages Of Testismentioning

confidence: 99%

Multipathway synergy promotes testicular transition from growth to spermatogenesis in early-puberty goats

Jiang

Liu

et al. 2020

BMC Genomics

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Background The microscopic process of postnatal testicular development in early-puberty animals is poorly understood. Therefore, in this study, 21 male Yiling goats with average ages of 0, 30, 60, 90, 120, 150 and 180 days old (each age group comprised three goats) were used to study the changes in organs, tissues and transcriptomes during postnatal testicle development to obtain a broad and deep insight into the dynamic process of testicular transition from growth to spermatogenesis in early-puberty animals. Results The inflection point of testicular weight was at 119 days postpartum (dpp), and the testicular weight increased rapidly from 119 dpp to 150 dpp. Spermatozoa were observed in the testis at 90 dpp by using haematoxylin–eosin staining. We found from the transcriptome analysis of testes that the testicular development of Yiling goat from birth to 180 dpp experienced three stages, namely, growth, transition and spermatogenesis stages. The goats in the testicular growth stage (0–60 dpp) showed a high expression of growth-related genes in neurogenesis, angiogenesis and cell junction, and a low expression of spermatogenesis-related genes. The goats aged 60–120 dpp were in the transitional stage which had a gradually decreased growth-related gene transcription levels and increased spermatogenesis-related gene transcription levels. The goats aged 120–180 dpp were in the spermatogenesis stage. At this stage, highly expressed spermatogenesis-related genes, downregulated testicular growth- and immune-related genes and a shift in the focus of testicular development into spermatogenesis were observed. Additionally, we found several novel hub genes, which may play key roles in spermatogenesis, androgen synthesis and secretion, angiogenesis, cell junction and neurogenesis. Moreover, the results of this study were compared with previous studies on goat or other species, and some gene expression patterns shared in early-puberty mammals were discovered. Conclusions The postnatal development of the testis undergoes a process of transition from organ growth to spermatogenesis. During this process, spermatogenesis-related genes are upregulated, whereas neurogenesis-, angiogenesis-, cell junction-, muscle- and immune-related genes are downregulated. In conclusion, the multipathway synergy promotes testicular transition from growth to spermatogenesis in early-puberty goats and may be a common rule shared by mammals.

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Postnatal testicular development and testosterone changes in Ghezel ram lambs

Cited by 15 publications

References 24 publications

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

Study of spermatogenic and Sertoli cells in the Hu sheep testes at different developmental stages

Multipathway synergy promotes testicular transition from growth to spermatogenesis in early-puberty goats

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