Postnatal proliferation of DRG non-neuronal cells in vitamin E-deficient rats

Cecchini, Tiziana; Ferri, Paola; Ciaroni, Sandra; Cuppini, Riccardo; Ambrogini, Patrizia; Papa, Stefano; Grande, Paolo Del

doi:10.1002/(sici)1097-0185(19991001)256:2<109::aid-ar1>3.0.co;2-t

Cited by 15 publications

(7 citation statements)

References 38 publications

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“…A subpopulation of SGCs in the adult DRG [16], vestibular and spiral ganglia [67], was found to be nestin‐positive. In the normal adult DRG, we found, similar to other reports, that SGCs incorporated BrdU [68, 69]. SGCs in neonatal DRG are multipotential precursors that can differentiate into oligodendrocytes, astrocytes, and Schwann cells [70].…”

Section: Discussionsupporting

confidence: 89%

Isolation and Characterization of Neural Crest Progenitors from Adult Dorsal Root Ganglia

2007

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After peripheral nerve injury, the number of sensory neurons in the adult dorsal root ganglia (DRG) is initially reduced but recovers to a normal level several months later. The mechanisms underlying the neuronal recovery after injury are not clear. Here, we showed that in the DRG explant culture, a subpopulation of cells that emigrated out from adult rat DRG expressed nestin and p75 neurotrophin receptor and formed clusters and spheres. They differentiated into neurons, glia, and smooth muscle cells in the presence or absence of serum and formed secondary and tertiary neurospheres in cloning assays. Molecular expression analysis demonstrated the characteristics of neural crest progenitors and their potential for neuronal differentiation by expressing a set of well-defined genes related to adult stem cells niches and neuronal fate decision. Under the influence of neurotrophic factors, some of these progenitors gave rise to neuropeptide-expressing cells and protein zero-expressing Schwann cells. In a 5-bromo-2-deoxyuridine chasing study, we showed that these progenitors likely originate from satellite glial cells. Our study suggests that a subpopulation of glia in adult DRG is likely to be progenitors for neurons and glia and may play a role in neurogenesis after nerve injury.

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Section: Discussionsupporting

confidence: 89%

Isolation and Characterization of Neural Crest Progenitors from Adult Dorsal Root Ganglia

2007

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“…The distribution of the cross‐sectional areas of the DRG cells innervating the nipples of lambs shows that these are cells of group I and II, belonging to the C and Aδ category, respectively. The finding that the DRG cells innervating the nipples of lambs are smaller than those of ewes is compatible with the observation that the size of the DRG cells increases during the development of the animal (Ohta et al, 1974; Larnicol et al, 1991, Cecchini et al, 1999). This finding as well as the frequency distribution of the cross‐sectional areas of the DRG cells after injections into the nipples of lambs resembles the results after injections into the pseudonipples.…”

Section: Discussionsupporting

confidence: 82%

Anatomic organization of the ascending branch of the milk‐ejection reflex in sheep: Primary afferent neurons

Tsingotjidou

Papadopoulos

2003

J of Comparative Neurology

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The present study examines the anatomic characteristics of the primary afferent neurons that innervate the nipples and pseudonipples of ewes and the nipples of lambs. For this purpose, horseradish-peroxidase coupled to wheat germ agglutinin (WGA-HRP) was injected intradermally into the whole extent, the tip, or the base of the nipples and pseudonipples, as well as into a region of the posterior surface of the udder. After survival periods of 72-96 hours, dorsal root ganglia (DRG), segments of the spinal cord and medulla oblongata were sectioned and reacted histochemically with tetramethylbenzidine to reveal the transganglionically transported tracer. Injections of WGA-HRP in the nipples and pseudonipples of the ewe resulted in labeled cells in the second to fifth ipsilateral lumbar spinal ganglia (L(2)-L(5)) and third and fourth (L(3) and L(4)) lumbar spinal ganglia, respectively. Labeled cells after WGA-HRP injections in the nipples of the lamb were found in the ipsilateral L(3)-L(5) spinal ganglia. Central projections of the DRG-labeled cells were found in the medial part of laminae I-III of the ipsilateral L(3) and L(4) spinal segments (ewe and lamb) and in the ipsilateral dorsal column nuclei (ewe). Central projections of the DRG-labeled cells after injections in the pseudonipples of the ewe were located in the medial part of laminae I-III of the ipsilateral L(3) spinal segment. The results of this study demonstrate that, whereas the innervation of the nipples of the ewe originates from four successive lumbar spinal ganglia (L(2)-L(5)), the innervation of the nipples of the lamb and the pseudonipples of the ewe originates from three (L(3)-L(5)) and two (L(3) and L(4)) successive ganglia, respectively.

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“…Like other glial cells, the SGC responds to nerve injury by upregulating glial fibrillary acidic protein (GFAP) expression and undergoing division (Humbertson et al , 1969; Elson et al , 2004b; Zhang et al , 2009) in response to injury (Gunjigake et al , 2009). Evidence that SGCs proliferate comes from the observation of mitotic figures (Humbertson et al , 1969) and BrdU positive cells surrounding ganglion neurons following the nerve damage (Cecchini et al , 1999; Elson et al , 2003, 2004a). One caveat to these studies is that specific markers were not used to unambiguously identify SGCs, rather they were recognized by morphological criteria alone.…”

Section: Introductionmentioning

confidence: 99%

Can satellite glial cells be therapeutic targets for pain control?

et al. 2010

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Satellite glial cells (SGCs) undergo phenotypic changes and divide the following injury into a peripheral nerve. Nerve injury, also elicits an immune response and several antigen-presenting cells are found in close proximity to SGCs. Silencing SCG-specific molecules involved in intercellular transport (Connexin 43) or glutamate recycling (glutamine synthase) can dramatically alter nociceptive responses of normal and nerve-injured rats. Transducing SGCs with glutamic acid decarboxylase can produce analgesia in models of trigeminal pain. Taken together these data suggest that SGCs may play a role in the genesis or maintenance of pain and open a range of new possibilities for curing neuropathic pain.

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Postnatal proliferation of DRG non-neuronal cells in vitamin E-deficient rats

Cited by 15 publications

References 38 publications

Isolation and Characterization of Neural Crest Progenitors from Adult Dorsal Root Ganglia

Isolation and Characterization of Neural Crest Progenitors from Adult Dorsal Root Ganglia

Anatomic organization of the ascending branch of the milk‐ejection reflex in sheep: Primary afferent neurons

Can satellite glial cells be therapeutic targets for pain control?

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