Postnatal Neuron Death in the Nodose Ganglia of the Rat

Bakal, Ron; Wright, Linda L.

doi:10.1159/000111312

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…There are only a few previously published estimates of the number of neurons in the rat nodose ganglia (Cooper, 1984;Bakal and Wright, 1993;Carobi, 1996;Chang et al, 2003;Wei et al, 2005). Our estimates for the total number of neurons in the nodose ganglia are in general agreement with most other investigators, who report numbers in the range of 5,000 -7,000 neurons per ganglion (Cooper, 1984;Carobi, 1996;Chang et al, 2003;Wei et al, 2005).…”

Section: Discussionsupporting

confidence: 90%

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat

2006

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Most vagal afferent neurons in rat nodose ganglia express mRNA coding for the NR1 subunit of the heteromeric N-methyl-D-aspartate (NMDA) receptor ion channel. NMDA receptor subunit immunoreactivity has been detected on axon terminals of vagal afferents in the dorsal hindbrain, suggesting a role for presynaptic NMDA receptors in viscerosensory function. Although NMDA receptor subunits (NR1, NR2B, NR2C, and NR2D) have been linked to distinct neuronal populations in the brain, the NMDA receptor subunit phenotype of vagal afferent neurons has not been determined. Therefore, we examined NMDA receptor subunit (NR1, NR2B, NR2C, and NR2D) immunoreactivity in vagal afferent neurons. We found that, although the left nodose contained significantly more neurons (7,603), than the right (5,978), the proportions of NMDA subunits expressed in the left and right nodose ganglia were not significantly different. Immunoreactivity for NMDA NR1 subunit was present in 92.3% of all nodose neurons. NR2B immunoreactivity was present in 56.7% of neurons; NR2C-expressing nodose neurons made up 49.4% of the total population; NR2D subunit immunoreactivity was observed in just 13.5% of all nodose neurons. Double labeling revealed that 30.2% of nodose neurons expressed immunoreactivity to both NR2B and NR2C, whereas NR2B and NR2D immunoreactivities were colocalized in 11.5% of nodose neurons. NR2C immunoreactivity colocalized with NR2D in 13.1% of nodose neurons. Our results indicate that most vagal afferent neurons express NMDA receptor ion channels composed of NR1, NR2B, and NR2C subunits and that a minority phenotype that expresses NR2D also expresses NR1, NR2B, and NR2C.

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Section: Discussionsupporting

confidence: 90%

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat

2006

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“…The numbers of total neurons determined in this study differ from numbers found in studies by Bakal and Wright (1993) and Cooper (1984). Bakal and Wright (1993) found that total neuron number in the nodose ganglion decreased between postnatal days 1 and 14, while Cooper (1984) noted that nodose neuron numbers remained constant from birth.…”

Section: Discussioncontrasting

confidence: 97%

“…Bakal and Wright (1993) found that total neuron number in the nodose ganglion decreased between postnatal days 1 and 14, while Cooper (1984) noted that nodose neuron numbers remained constant from birth. The current study showed that neuron numbers rise between PD6 and PD10, and then stabilize (Figure 3A).…”

Section: Discussionmentioning

confidence: 99%

Early postnatal ozone exposure alters rat nodose and jugular sensory neuron development

Zellner¹,

Brundage²,

Hunter³

et al. 2011

Toxicological & Environmental Chemistry

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Sensory neurons originating in nodose and jugular ganglia that innervate airway epithelium (airway neurons) play a role in inflammation observed following exposure to inhaled environmental irritants such as ozone (O3). Airway neurons can mediate airway inflammation through release of the neuropeptide substance P (SP). While susceptibility to airway irritants is increased in early life, the developmental dynamics of afferent airway neurons are not well characterized. The hypothesis of this study was that airway neuron number might increase with increasing age, and that an acute, early postnatal O3 exposure might increase both the number of sensory airway neurons as well as the number SP–containing airway neurons. Studies using Fischer 344 rat pups were conducted to determine if age or acute O3 exposure might alter airway neuron number. Airway neurons in nodose and jugular ganglia were retrogradely labeled, removed, dissociated, and counted by means of a novel technique employing flow cytometry. In Study 1, neuron counts were conducted on postnatal days (PD) 6, 10, 15, 21, and 28. Numbers of total and airway neurons increased significantly between PD6 and PD10, then generally stabilized. In Study 2, animals were exposed to O3 (2 ppm) or filtered air (FA) on PD5 and neurons were counted on PD10, 15, 21, and 28. O3 exposed animals displayed significantly less total neurons on PD21 than FA controls. This study shows that age-related changes in neuron number occur, and that an acute, early postnatal O3 exposure significantly alters sensory neuron development.

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Gastrointestinal projection maps of the vagus nerve are specified permanently in the perinatal period

Powley

Martinson

Phillips

et al. 2001

Developmental Brain Research

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Postnatal Neuron Death in the Nodose Ganglia of the Rat

Cited by 5 publications

References 14 publications

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat

Early postnatal ozone exposure alters rat nodose and jugular sensory neuron development

Gastrointestinal projection maps of the vagus nerve are specified permanently in the perinatal period

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