“…Ex vivo approaches have been used most frequently to evaluate BAPN-induced AAD in mice ( Fig 2 , C ). 12 , 47 , 48 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 63 , 64 , 79 BAPN induces heterogeneous aortic pathologies in the entire thoracic aorta. Because ex vivo approaches can detect AAD directly in any region, it has advantages for the evaluation of aortic pathologies in BAPN-administered mice.…”