Postnatal characteristics of calves produced by nuclear transfer cloning

Garry, Franklyn B.; Adams, R.; McCann, J P; Odde, K. G.

doi:10.1016/0093-691x(95)00363-d

Cited by 164 publications

(111 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Em alguns destes estudos foi observado peso ao nascimento superior em animais concebidos por biotécnicas artificiais (Garry et al 1996, Kruip & den Daas 1997, Jacobsen et al 2000, Batchelder et al 2007, Meirelles et al 2010.…”

Section: Resultsunclassified

“…aliadas ao gigantismo, são bem caracterizadas nos bezerros clonados, assim como o maior tamanho dos vasos umbilicais e a maior incidência de doenças envolvendo essas estruturas (Garry et al 1996, Batchelder et al 2007, Miglino et al 2007, Meirelles et al 2010). Nos produtos de PIVE isso não está bem elucidado.…”

Section: Resultsunclassified

“…Como evidenciado na rotina prática, esses bezerros podem nascer com os vasos umbilicais mais calibrosos, os quais se atrofiam mais lentamente. No caso dos bezerros clonados já está bem caracterizado que nascem com tamanho e peso maiores, com vasos umbilicais de maior diâmetro e apresentam maior chance de desenvolverem doenças umbilicais (Garry et al 1996, Batchelder et al 2007, Miglino et al 2007, Meirelles et al 2010. A ocorrência da síndrome do bezerro grande (Large Offspring Syndrome) é comumente descrita em animais frutos de processos artificiais de fertilização e não somente nos bezerros clonados (Behboodi et al 1995, Kruip & den Daas 1997, Young et al 1998, Jacobsen et al 2000, Bertolini & Anderson 2002, Farin et al 2006.…”

Section: Introductionunclassified

See 2 more Smart Citations

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

Sturion

Lisbôa

2013

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Esse trabalho foi desenvolvido com o objetivo de caracterizar a involução das estruturas umbilicais em bezerros sadios da raça Nelore ao longo dos primeiros 35 dias de vida, e de comparar esse processo em bezerros concebidos por métodos naturais ou por fertilização in vitro (FIV). Quarenta bezerros foram distribuídos em dois grupos (n=20) de acordo com o método de concepção (natural ou FIV) e cada grupo foi composto por dez machos e dez fêmeas. A ultrassonografia (transdutor microconvexo de 7,5 MHz) foi empregada para examinar o conjunto das estruturas remanescentes do cordão umbilical que compõem o umbigo externo e as estruturas abdominais (veia umbilical, artéria umbilical esquerda e ducto alantóide), mensurando-se os seus diâmetros em locais definidos. Os exames foram realizados entre 24 e 36 horas de vida e aos 7, 14, 21, 28 e 35 dias de idade. Testaram-se os efeitos do sexo, da idade e do método de concepção por meio da análise de variâncias de medidas repetidas. O exame ultrassonográfico provou-se adequado para a avaliação das estruturas umbilicais extra e intracavitárias permitindo a caracterização do processo fisiológico de involução das mesmas. No umbigo externo, as veias umbilicais foram observadas como imagem individualizada até os 14 dias de vida e um conjunto de estruturas em processo de atrofia era visualizado após essa idade. No abdômen, a veia e a artéria umbilicais foram visualizadas até os 35 dias de idade e o ducto alantóide somente durante a primeira semana de vida. Essas estruturas apresentaram-se com parede hiperecóica regular e contínua e lúmen homogeneamente anecóico. O diâmetro de todas as estruturas umbilicais estudadas se reduziu continuamente ao longo do primeiro mês de vida (p<0,05), sem efeito do sexo (p>0,05). Comparados aos bezerros concebidos por métodos naturais, os produtos de FIV nasceram com os vasos umbilicais e o ducto alantóide um pouco mais calibrosos (diâmetros 1 a 3 mm maiores). Distintamente dos valores mais elevados estabelecidos em estudos prévios para os bezerros de raças européias, pode-se admitir, por fim, que nos bezerros recém-nascidos sadios da raça Nelore a espessura das estruturas que compõem o umbigo externo não deve ultrapassar 2 cm, o diâmetro da veia e da artéria umbilicais pode chegar a 1 cm e o do ducto alantóide é próximo a 0,5 cm.

show abstract

Section: Resultsunclassified

Section: Introductionunclassified

See 1 more Smart Citation

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

Sturion

Lisbôa

2013

Pesq. Vet. Bras.

View full text Add to dashboard Cite

show abstract

“…Morphologically, they showed successful reprogramming without any defects at least until the pre-implantation stage. However, it is widely acknowledged that nuclear transfer, even with embryonic blastomeres, results in increased rates of abortion throughout pregnancy, high birth weight, perinatal deaths, and poor adaptation to extra-uterine life [2,4,11]. These effects appear more extreme with somatic cell nuclear transfer [1, Second, due to the relatively small size of HanWoo calves, the chance of dystocia by large calf derived from in vitro produced embryos can be reduced, even in twin pregnancies [5].…”

Section: Discussionmentioning

confidence: 99%

Production of HanWoo(Bos taurus coreanae) Fetuses Following Interbreed Somatic Cell Nuclear Transfer.

Roh

Yoon

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The possibility of producing HanWoo (Bos taurus coreanae) calves from transferable bovine embryos, obtained by interbreed nuclear transfer using Holstein cytoplasts and surrogates, was investigated. As donor nuclei, HanWoo fetal fibroblasts were used. Cells were induced into quiescence by serum deprivation for 4-7 days before nuclear transfer. In vitro matured Holstein oocytes were enucleated, and single donor cell was placed into the perivitelline space of enucleated oocyte. After reconstruction, the embryos were fused, activated and cultured. On day 7, the embryos that developed to the blastocyst stages were transferred into Holstein recipient cows on day 6 to 7 of estrous cycle (estrus=0). The reconstructed embryos were successfully fused (58.8%; 47/80), cleaved (91.5%; 43/47), and developed to blastocysts (29.8%; 14/47). Eleven blastocysts were transferred into 5 Holstein recipient cows. Two recipients were pregnant, confirmed by ultrasonography at day 60 of gestation. But, one of them was opened between on day 80 to 100 of pregnancy, and the other had a stillbirth on day 255. The stillborn calf was physically normal, and we couldn't find any evidence of anomaly. These results show that cells derived from HanWoo somatic cell lines can be reprogrammed by interbreed nuclear transfer and develop subsequently in vivo as well as in vitro. KEY WORDS: HanWoo (Bos taurus coreanae), interbreed, nuclear transfer.J. Vet. Med. Sci. 63(9): 945-948, 2001 Production of cloned animal by somatic cell nuclear transfer has recently been developed. The birth of live offspring derived from embryos reconstructed with somatic cells have been reported in several species; sheep [10], cattle [1,3,8], and mouse [7].Animal cloning technology can be used to preserve endangered mammalian species by interbreed or interspecies nuclear transfer. The success of interbreed nuclear transfer was reported in last surviving cow of the Enderby island cattle breed [9]. HanWoo (Bos taurus coreanae) inhabits only in the region of Korea peninsula and becomes endangered animal in the bovine species. To produce HanWoo more efficiently, we developed in vitro production and embryo transfer system and produced HanWoo twin calves from the Holstein recipient cows [5]. Due to the relatively small size of HanWoo calves, we could obtain twins easily without dystocia or other problems during the pregnancy.In this study, we used Holstein recipient cytoplast for the production of HanWoo nuclear transfer embryos, and transfer them into Holstein cows to obtain cloned HanWoo offspring. MATERIALS AND METHODS Preparation of fetal fibroblasts:Fibroblast cells were obtained from HanWoo conceptuses on Day 50 to 60 of pregnancy. The head, extremities and internal organs were removed before tissues were cut into small pieces, and cells were dispersed by exposure to 0.25% (w/v) trypsin solution (Gibco BRL, Grand Island, NY). Cells were cultured in Dulbeco's minimum essential medium (Gibco BRL) supplemented with 10% fetal bovine serum (FBS, Gibco BRL). For each p...

show abstract

“…Research using SCNT technology suggested that abnormal expression of genes during the earliest stages of development could be the reason of embryonic losses (Arnold et al 2006). Abnormal gene expression might be accountable for the observed phenotypes in embryos produced by SCNT, not only during early embryonic development, but also during gestation and parturition leading in many cases to the so called abnormal offspring syndrome (AOS; Garry et al 1996;Ranilla et al 1998;Zhang et al 2004). Aberrant gene reprogramming after NT has been documented also for miRNA ).…”

Section: Introductionmentioning

confidence: 99%

A comparative study on expression profile of developmentally important genes during pre-implantation stages in buffalo hand-made cloned embryos derived from adult fibroblasts and amniotic fluid derived stem cells

Shah

Kataria

et al. 2015

Cytotechnology

View full text Add to dashboard Cite

Abnormal gene expression in somatic cell nuclear transfer embryos due to aberrant epigenetic modifications of the donor nucleus may account for much of the observed diminished viability and developmental abnormalities. The present study compared the developmentally important gene expression pattern at 4-cell, 8-to 16-cell, morula, and blastocyst stages of buffalo nuclear transfer (NT) embryos from adult fibroblasts (AFs) and amniotic fluid stem cells (AFSCs). In vitro fertilized embryos were used as control embryos. Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling, and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), oxidative stress (MnSOD), metabolism (GLUT1) regulation were observed in cloned embryos. The expression of transcripts in AFSC-NT embryos more closely followed that of the in vitro fertilized embryos compared with AF-NT embryos. It is concluded that AFSCs with a relatively undifferentiated genome may serve as suitable donors which could be reprogrammed more efficiently to reactivate expression of early embryonic genes in buffalo NT.

show abstract

Postnatal characteristics of calves produced by nuclear transfer cloning

Cited by 164 publications

References 20 publications

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

Production of HanWoo(Bos taurus coreanae) Fetuses Following Interbreed Somatic Cell Nuclear Transfer.

A comparative study on expression profile of developmentally important genes during pre-implantation stages in buffalo hand-made cloned embryos derived from adult fibroblasts and amniotic fluid derived stem cells

Contact Info

Product

Resources

About