Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Gye, Myung Chan; Kim, Chun; Ahn, Hyun Soo; Kim, Y S

doi:10.1080/01485010150211155

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…This suggests that Ca 2þ homeostasis through the T-type Ca 2þ channel, as well as L-type Ca 2þ channel, is crucial for normal spermatogenesis. During the late stage of spermatogenesis, round spermatids undergo substantial changes in morphology, rearrangements in the composition and function of a number proteins including regulator for the intracellular concentration of Ca 2þ and Ca 2þ -binding proteomes [12,28,30]. Of these, protamines, as well as transition proteins, which are crucial for nuclear remodeling, are expressed in postmeiotic germ cells under control of transcription factor cAMP-responsive element modulator (CREM) [22].…”

Section: Discussionmentioning

confidence: 99%

Effects of Calcium Channel Blockers on the Spermatogenesis and Gene Expression in Peripubertal Mouse Testis

Lee

Kim

et al. 2006

Archives of Andrology

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Treatment of Ca(2+) channel blockers (CCB) to relieve hypertension causes reversible male infertility, suggesting deregulation of Ca(2+) homeostasis in testis is closely related with male infertility. To investigate the possible toxicity of therapeutic application of CCB in childhood, the effect of nifedipine and ethosuximide, an L-type and T-type CCB, respectively, on the spermatogenesis and testicular gene expression was examined. Following the intraperitoneal injection of either drug for 7 days to 18 days on old mice, the paired testes weights were significantly lower in mice treated with nifedipine (> or = 10 mg/kg/day) or ethosuximide (100 mg/kg/day) than vehicle controls. In mice given high drug dosing (100 mg/kg), seminiferous tubules showed immaturity with spermatogenic arrest at elongating spermatid stage and poorly developed lumen. Unexpectedly, the expression of activator isoform of transcription factor cAMP-responsive element modulator (CREM) mRNA increased together with transition protein 2 and protamine 2 mRNA in drug-treated mice testes, suggesting that CCB may deregulate expression of activator isoform of CREM in male germ cells and that spermatogenic defect following CCB treatment may attribute to ectopic expression of CREM-dependent gene battery in testis. Therapeutic application of CCB in childhood should be cautious because of their potential to cause spermatogenic defect and altered gene expression in testis.

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Section: Discussionmentioning

confidence: 99%

Effects of Calcium Channel Blockers on the Spermatogenesis and Gene Expression in Peripubertal Mouse Testis

Lee

Kim

et al. 2006

Archives of Andrology

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“…Direct relation of the increase in expression of CREST in pancreatic β-cells of hyperglycemic rats to high glucose-induced apoptosis suggests multifunction of CREST in non-nervous tissues (Men et al 2013). Interestingly, crest mutant mice often develop infertility (Aizawa et al 2004) and a series of proteins interacting with CREST are found closely related to the differentiation and maturation of the spermatogenic epithelial cells (Gye et al 2001;Don and Stelzer 2002;Boussouar et al 2014;Carre et al 2018). Histone acetyltransferases p300 and CREB-binding protein (CBP), two of the earliest identified CREST-interacting proteins, regulate the determination and differentiation of testicular tissues as well as the metabolic remodeling in the later stages of spermatogenesis (Gye et al 2001;Don and Stelzer 2002;Boussouar et al 2014;Carre et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes

Jiao

et al. 2021

Histochem Cell Biol

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Calcium-responsive transactivator (CREST), a nuclear protein highly expressed in postmitotic neurons, is involved in the regulation of cell cycle, differentiation and dendritic development of neuronal cells. Its mRNA has been detected in the testis of adult rat, whilst its protein expression and distribution pattern in the testis remain to be elucidated. In this study, we examined the distribution of CREST in the adult testes of both rats and human as well as the expression pattern of CREST in the testes of postnatal developing rats. In the adult testes of both human and rats, immunohistochemical analysis revealed that CREST was selectively distributed in the mature Sertoli cells but not in the spermatogenic cells. In the testes of postnatal developmental rats, CREST was expressed not only in Sertoli cells but also in the gonocytes and spermatogenic cells at the initial stage of spermatogenic cell differentiation. CREST immunoreactivity continued to increase in Sertoli cells during differentiation, reaching its peak in adulthood. However, CREST immunostaining intensity dramatically decreased as the spermatogenic cells differentiate, disappearing in the post-differentiation stage. Furthermore, Brg1 and p300, two CREST-interacting proteins ubiquitously expressed in the body, are found to be colocalized with CREST in the spermatogenic epithelial cells including Sertoli cells. The unique expression pattern of CREST in developing testis suggests that CREST might play regulatory roles in the differentiation of spermatogenic epithelial cells. The Sertoli cell-specific expression of CREST in the adulthood hints that CREST might be a novel biomarker for the mature Sertoli cells.

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Stage‐specific and tissue‐specific expression characteristics of differentially expressed genes during mouse spermatogenesis

Guo

Guan

et al. 2004

Molecular Reproduction Devel

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Spermatogenesis occurs in successive mitotic, meiotic, and post-meiotic phase, and involves a number of unique processes including meiosis and dramatic morphological changes. The unique differentiation mechanisms of spermatogenesis suggest the existence of germ-cell-specific molecules. The most straight forward strategy to elucidate differentiation mechanisms is to identify and characterize differentiation-specific molecules and their associated genes in germ cells. However, only a few genes specifically involved in spermatogenesis have been studied. In the present study, six different types of spermatogenic cells (primitive type A spermatogonia, type B spermatogonia, preleptotene spermatocytes, pachytene spermatocytes, round spermatids, and elongating spermatids) were isolated from Balb/c mice testes using velocity sedimentation and Atlas cDNA arrays containing 1,176 known mouse genes were used to determine the gene expression profiles of the spermatogenic cells. The expression of 260 genes were detected in six different stages of spermatogenic cells and a number of genes showed differential expression. The 23 differentially expressed genes were further analysed by reverse transcription polymerase chain reaction (RT-PCR) for their stage-specific and tissue-specific expression characteristics. Based on the results of RT-PCR, six genes highly express in both primitive type A and type B spermatogonia, four genes up-regulate in type B spermatogonia, two genes up-regulate in spermatocytes, two genes up-regulate in spermatids, three genes express constantly from primitive A spermatogonia to elongating spermatids, two genes express constantly from primitive A spermatogonia to round spermatids, two genes do not change in their expression during spermatogenesis, two genes can be detected highly in adult testis, but are undetectable in spermatogenic cells. The tissue-specific expression characteristics of the 23 genes showed that some of them specifically expressed in testes or other tissues. These data provide new information for further studies into spermatogenesis-related genes and may lead to the identification of genes with potential relevance to the differentiation of spermatogenic cells. In addition, some of these genes could be considered to be used as the molecular markers for different stages of spermatogenic cells.

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Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Cited by 3 publications

References 16 publications

Effects of Calcium Channel Blockers on the Spermatogenesis and Gene Expression in Peripubertal Mouse Testis

Effects of Calcium Channel Blockers on the Spermatogenesis and Gene Expression in Peripubertal Mouse Testis

Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes

Stage‐specific and tissue‐specific expression characteristics of differentially expressed genes during mouse spermatogenesis

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