Postnatal Changes in Oxygen Transport of Term, Premature, and Sick Infants: The Role of Red Cell 2,3-Diphosphoglycerate and Adult Hemoglobin

Delivoria‐Papadopoulos, Maria; Roncević, N; Oski, Frank A.

doi:10.1203/00006450-197106000-00001

Cited by 205 publications

(60 citation statements)

References 22 publications

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“…2,3-DPG reduces oxygen affinity either by stabilizing the quaternary structure of Hb or by altering the intraerythrocytic pH relative to plasma pH. Both mechanisms apply in the intact erythrocyte, and at concentrations of 2,3-DPG above normal the latter predominates [16,17]. HbF has decreased interaction with 2,3-DPG.…”

Section: Discussionmentioning

confidence: 99%

“…Levels of lactate and 2,3-DPG were measured enzymatically after immediate deproteinization of the blood sample (Sigma Diagnostics) [11,12]. Functional fraction of 2,3-DPG (FF 2,3-DPG) and partial pressure of O 2 at 50% saturation of Hb (P 50 ) were estimated indirectly as proposed by Delivoria et al [15][16][17] and Orzalesi et al [18,19] respectively.…”

Section: Methodsmentioning

confidence: 99%

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Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Soubasi¹,

Kremenopoulos²,

Tsantali

et al. 2000

Neonatology

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The aim of this study was to investigate the effect of recombinant human erythropoietin (rHu-EPO) on oxygen affinity and adequate oxygen delivery to the tissues of stable premature infants. 36 very-low-birth-weight infants were randomly assigned to either receive rHu-EPO (200 units/kg every other day) or not, and both groups were supplemented with iron, folic acid and vitamin E. Arterial blood gases, oxygen saturation, complete blood counts, fetal haemoglobin, 2,3-diphosphoglycerate (2,3-DPG) and blood lactate were analysed weekly, from the 1st week till discharge. Patients in the two groups were comparable. There was a trend in increasing lactate values towards the 4th to 5th weeks of life, which did not reach statistical significance. There was no correlation between lactate values and the studied variables (pH, BE, oxygen saturation). In 35 transfusions, pre- and 24 h post-transfusion blood lactate status was studied. In 23 of them, a decrease in post-transfusion lactate was noticed, whilst an increased post-transfusion level was shown in 10 cases and no change in 2 cases. The mean pre-transfusion lactate value was significantly higher than the post-transfusion one (24.04 ± 11.9 mg/dl before and 16.27 ± 8.5 mg/dl after transfusion; p = 0.0025). In both groups there was a steady rise in 2,3-DPG concentration over the period of study, and the 2,3-DPG values at the end of our study were significantly increased in the rHu-EPO group (rHu-EPO 5.98 ± 0.9, control 4.84 ± 0.7; p = 0.04). In conclusion, the use of rHu-EPO did not affect blood lactate levels compared to the control group. Regarding oxygen affinity, it seems that rHu-EPO causes a shift of the oxy-haemoglobin dissociation curve to the right. This is a previously unreported effect of rHu-EPO and its clinical use may, thus, confer to preterm babies an added advantage.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Soubasi¹,

Kremenopoulos²,

Tsantali

et al. 2000

Neonatology

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“…The extra-uterine life, with normal oxygen tension, forces circulating cells to modify themselves; in fact, their capacity to free oxygen to tissues is al ready augmented during the 1st day of life [Delivoria-Papadopoulos et al, 1971]; these modifications will probably induce a nat ural selection, promoting those surface changes, including sialic acid decrement, which result in the sequestration of a num ber of cells. That could explain the tran sient rise of sialylation in the 2nd week, when most of the cells with a low sialic acid content have been eliminated, and the mean value rises; then it decreases again, since fetal cells are ageing and are not re placed by new erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte Membrane Sialic Acid in New-Born Infants

Calatroni¹,

Cordaro

Salpietro

et al. 1984

Acta Haematol

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Erythrocyte surface sialic acid was measured in 94 full-term new-born infants and in 20 premature infants of different ages, varying from 1 to 16 post-natal days. After the first 3 days of life, the amount of sialic acid, both per cell and per gramme of haemoglobin, was rapidly reduced to 70–80% of the initial value; these lower levels were maintained on the following days. The decrease observed on the 4th day may represent an expression of the mechanism by which a large amount of fetal red blood cells is eliminated from the circulation during the same period. Therefore, the relationship between sialic acid decrement and red cell sequestration exists also in fetal erythrocytes, and this may contribute to clarify the exact role of sialic acid in red cell survival time.

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“…Pooled blood from a group of 7 mice was obtained following 6 days exposure to hyperoxia and 48 h after the second 19-nortestosterone decanoate (19-ND) injec tion, and RBC 2,3-DPG and the partial pressure at which 50% of the hemoglobin is oxygenated (PJ0) were measured according to the method of Delivorla-Papadopoulos et al [7], Nortestosterone decanoate was kindly supplied by Dr. Henry Strade (Organon, West Orange, N.J.) in a concentration of 25 mg/ml dissolved in propylene glycol. Injections were given on the 4th and 5th hyperoxic day.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hyperoxia and Androgen on Red Cell 2,3-Diphosphoglycerate and Oxygen Affinity

Gorshein¹,

Gardner²,

Tyree³

et al. 1976

Acta Haematol

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Increased plasma erythropoietin (ESF) activity, a rise in RBC 2,3-DPG, and a ‘right shift’ of the oxygen hemoglobin equilibrium curve following androgen administration to mice in ambient or hyperoxic conditions, was demonstrated. If androgens had a direct effect on the RBC metabolism, increased 2,3-DPG would result in a facilitated release of oxygen to the tissue. This would have been accompanied by a decrease rather than an increase in the level of ESF. Hyperoxia has abolished detectable rising levels of plasma ESF and RBC 2,3-DPG following androgen administration. These levels were close to those seen in the ambient nontreated mice.

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Postnatal Changes in Oxygen Transport of Term, Premature, and Sick Infants: The Role of Red Cell 2,3-Diphosphoglycerate and Adult Hemoglobin

Cited by 205 publications

References 22 publications

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Erythrocyte Membrane Sialic Acid in New-Born Infants

Effect of Hyperoxia and Androgen on Red Cell 2,3-Diphosphoglycerate and Oxygen Affinity

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