“…The immunohistochemical staining was quantified as an area in square micrometers (maximum area for whole screen was 307,200 sq micrometers at 100× magnification) occupied by the densities of peroxidase staining above the sample background, as described previously [25,26]. Briefly, the illumination of empty field (image background) was first adjusted to 40 in 0 to 255 scale.…”
Background: Pelvic floor disorders affect almost 50% of aging women. An important role in the pelvic floor support belongs to the levator ani muscle. The p27/kip1 (p27) protein, multifunctional cyclin-dependent kinase inhibitor, shows changing expression in differentiating skeletal muscle cells during development, and relatively high levels of p27 RNA were detected in the normal human skeletal muscles.
“…The immunohistochemical staining was quantified as an area in square micrometers (maximum area for whole screen was 307,200 sq micrometers at 100× magnification) occupied by the densities of peroxidase staining above the sample background, as described previously [25,26]. Briefly, the illumination of empty field (image background) was first adjusted to 40 in 0 to 255 scale.…”
Background: Pelvic floor disorders affect almost 50% of aging women. An important role in the pelvic floor support belongs to the levator ani muscle. The p27/kip1 (p27) protein, multifunctional cyclin-dependent kinase inhibitor, shows changing expression in differentiating skeletal muscle cells during development, and relatively high levels of p27 RNA were detected in the normal human skeletal muscles.
“…In addition, new granulosa cell nests should be formed and descent with the assistance of MDC from the surface into the lower ovarian cortex as well (Bukovsky et al, 1995;Bukovsky et al, 2004). Hence, when compared to laboratory rodents, which exhibit during adulthood conditions similar to human fetal ovaries, the follicular renewal in adult humans is a much more complex process and OSC niche more sensitive to its alteration, with more frequently occurring POF as compared to the laboratory rodents, where such condition can be induced during the postnatal adaptive period (first week of postnatal life in rats and mice) by alteration of early ovarian development with sex steroids (Bukovsky et al, 2000.…”
Section: Ovarian Stem Cell Niche In Adult Human Ovaries Origin Of Newmentioning
Stem cell niche consists of perivascular compartment, which connects the stem cells to the immune and vascular systems. During embryonic period, extragonadal primordial germ cells colonize coelomic epithelium of developing gonads. Subsequently, ovarian stem cells (OSC) produce secondary germ cells under the influence of OSC niche, including immune system-related cells and hormonal signaling. The OSC in fetal and adult human ovaries serve as a source of germ and granulosa cells. Lack of either granulosa or germ cell niche will result in premature ovarian failure in spite of the presence of OSC. During perinatal period, the OSC transdifferentiate into fibroblast-like cells forming the ovarian tunica albuginea resistant to environmental threats. They represent mesenchymal precursors of epithelial OSC during adulthood. The follicular renewal during the prime reproductive period (PRP) ensures that there are fresh eggs available for a healthy progeny. End of PRP is followed by exponentially growing fetal genetic abnormalities. The OSC are present in adult, aging, and postmenopausal ovaries, and differentiate in vitro into new oocytes. During in vitro development of large isolated oocytes reaching 200 lm in diameter, an ancestral mechanism of premeiotic nurse cells, which operates during oogenesis in developing ovaries from invertebrates to mammalian species, is utilized. In vitro developed eggs could be used for autologous IVF treatment of premature ovarian failure. Such eggs are also capable to produce parthenogenetic embryos like some cultured follicular oocytes. The parthenotes produce embryonic stem cells derived from inner cell mass, and these cells can serve as autologous pluripotent stem cells. Anat Rec, 294:1284Rec, 294: -1306Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.
“…EE ( g/kg/day) aged rats (Bukovsky et al, 2000). Some ovaries from animals in the control group developed cystic follicles and lacked corpus luteum.…”
-Xenoestrogen exposure during the critical period of sexual differentiation of the brain causes delayed effects on female reproduction. We investigated the internal dose of orally administered ethynylestradiol (EE) during the critical period and its delayed effects by administering 0 (vehicle control), 0.4, or 2 μg/kg EE to female Sprague-Dawley rats for 5 days from postnatal day (PND) 1. Determination of serum EE level 24 hr after the initial dosing and 6 and 24 hr after the final dosing of 2 μg/kg indicated that the administered EE entered the circulation and cleared after every administration. Although the treatment did not affect physical development, including growth, eyelid opening, and vaginal opening, the estrous cycle was arrested from postnatal week (PNW) 12 even with 0.4 μg/kg EE, with an inverse correlation between doses and arresting ages. Although ovarian morphology at PNW 22-23 indicated that the treatment caused long-term anovulation and cystic follicle formation, the number of primordial follicles at PNW 22-23 was similar among the groups. Because this number was lower than that at PND 10 in all groups, primordial follicles may have been consumed under long-term anovulation. The treatment also caused other abnormalities, including mammary gland hyperplasia, increase in pituitary and liver weights, and decrease in the uterine weight. Because the highest circulating EE level in the 2 μg/kg-treated neonates is considered to be comparable to the physiological range of estradiol-17β, we concluded that a slight increase in the circulating estrogens during the neonatal period exerts irreversible delayed effects.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.