Postlaminectomy bone and scar formations in presence of ankaferd blood stopper and bitter melon (momordica charantia); an experimental study

Abstract

ABSTRACTobserved after laminectomy and revealed by magnetic resonance imaging (MRI) (22). Recently, more attention has been focused on PF, which may be responsible for recurrent pain after laminectomy (9,16) to prevent post laminectomy scarring and to induce new bone formation in different approaches (5,8,30). A number of synthetic and natural materials have been used to prevent or reduce PF and induce new bone formation after laminectomy in both animal and human studies (22). Especially, the PASS principles (Primary wound closure/ █ INTRODUCTION P eridural fibrosis (PF) is an undesirable outcome of decompressive spinal surgery that may compress the dura and nerve roots (3). PF develops out of the laminectomy membrane, which covers the defect created by the bone resection to replace the resected areas. Commonly, the bone defects as a result of laminectomy regenerate as fibrous tissue that intends to regenerate resected lamina (24). Briefly; PF is a part of the natural healing process and it could be AIm: A quantitative model of postlaminectomy was designed in rats. The effects of Momordica Charantia (MC) and Ankaferd blood stopper (ABS) on the bone and scar formation after laminectomy were concurrently evaluated. mATERIAl and mEThODS: Eighteen adult Wistar albino rats underwent lumbar laminectomy at L2-L3 vertebral levels, and were randomly assigned to one of three groups of six rats each. The Treatment group received MC and ABS treatment and the Control group was left untreated. Rats were sacrificed 4 weeks after treatment. Then; the lumbar spine was excised en-block, fixed and decalcified. Sections were stained with hematoxylin and eosin (H&E) and Masson's trichrome, and evaluated for peridural fibrosis (PF), new bone formation, and vascular proliferation. RESUlTS:Total volume of new bone in the MC group was significantly increased in comparison to the Control group (p<0.05). Also; there was highly significant increase in terms of the total volume of fibrous tissue in the MC and ABS groups when compared with the Control group (p<0.01). Besides; there was a highly significant difference between the MC and the Control groups (p<0.01) in point of total volume of vessel.CONClUSION: Both MC and ABS are not convenient to prevent the PF formation and MC may promote new bone formation and angiogenesis after lumbar laminectomy in rats.

A novel rat model of vertebral inflammation–induced intervertebral disc degeneration mediated by activating cGAS/STING molecular pathway

“…Sections were subjected to haematoxylin‐eosin (HE), safranin O/fast green (SOFG) and Masson's trichrome histological stainings, according to conventional procedures 8 , 29 and using standard HE, SOFG and Masson dye solutions (Wuhan Servicebio Technology Co., Ltd.). Microscope images were kept at different magnifications, using a scanning imaging system (ECLIPSE E100 and DS‐U3, NIKON).…”

<i>Momordica charantia</i> Enhances Tendon Healing in Rats: An Experimental Study

“…Kuruoğlu et al used MC extract in their study to improve bone and connective tissue stroma degeneration following experimental laminectomy. The researchers reported that MC extract application has been useful following bone and connective tissue injury, and the effect possibly resulted from increased collagen biosynthesis and TGF-β levels [Kuruoglu et al, 2017]. Previous studies have reported that treatment with MC significantly enhanced wound closure in diabetic rats and upregulated TGF-β expression in wounds, playing an essential role in regulating cell growth and differentiation [Hussan et al, 2014].…”

Evaluation of the effects of Ankaferd haemostat application on bone regeneration in rats with calvarial defects: histochemical, immunohistochemical and scintigraphic study

“…In previous histological studies investigating the effects of ABS on the bone tissue, it was found to have an acceleratory effect on the short-term bone healing process in samples taken on the 7th postoperative day [16,27]. Recently, Kuruoğlu et al (2017) evaluated the late effects of the combination of ABS and β-tricalcium phosphate (TCP) using immunohistochemical methods and they found the bone healing effects of ABS hemostat in their study [17]. Afterwards, Tanık et al (2018) also investigated the late period effects of the combination of ABS and β-TCP on the regeneration of bone tissue in rats [29].…”