Postischemic Intravenous Administration of Magnesium Sulfate Inhibits Hippocampal CA1 Neuronal Death after Transient Global Ischemia in Rats

Miles, Andrew N.; Majda, Bernadette T.; Meloni, Bruno P.; Knuckey, Neville W.

doi:10.1097/00006123-200112000-00025

Cited by 47 publications

(17 citation statements)

References 30 publications

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“…We also used MgSO 4 . Similar to our results, previous studies [29][30][31] have also found that MgSO 4 treatment had a neuroprotective effect in cerebral ischemia.…”

Section: Discussionsupporting

confidence: 93%

Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels after Cerebral Ischemia

Barışkaner¹,

Üstün

Ak³

et al. 2003

Pharmacology

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In the present study, the effects of magnesium sulfate on tissue lactate and malondialdehyde (MDA) levels after cerebral ischemia in rabbits were studied. The rabbits were divided equally into three groups. Group 1 (n = 8) was the sham-operated control group, in group 2 (n = 8) only cerebral ischemia was induced by clamping bilaterally the common carotid arteries for 60 min, and in group 3 (n = 8) magnesium sulfate was administered at a dose of 100 mg/kg i.v. within 5 min after opening the clamps. In group 1 EEG recordings were obtained immediately and 60 and 120 min after craniectomy. In groups 2 and 3 EEG recordings were obtained immediately after craniectomy but before clamping and 60 min after clamping. One hour after opening the clamps and taking EEG recordings, brain cortices were resected, and the concentrations of lactate and MDA were determined using spectrophotometric/enzymatic and thiobarbituric acid methods, respectively. In all groups, there were significant differences between MDA and lactate levels (p < 0.05). There were no significant differences in lactate levels between groups 2 and 3 (p > 0.05), and also the preischemic EEG grades were the same in all groups. Preischemic and postischemic EEG values were significantly different (p < 0.05), and there were also significant differences between postischemic EEG grades in groups 2 and 3 (p < 0.05). There was a correlation between postischemic EEG grades and MDA and lactate levels. These results demonstrate that cerebral ischemia-reperfusion injury leads to an increase in brain tissue lactate and MDA levels, that magnesium sulfate suppresses the increase of MDA and lactate concentrations, and that magnesium sulfate treatment improves the EEG changes. The EEG grades correlated well with MDA and lactate levels.

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“…We also used MgSO 4 . Similar to our results, previous studies [29][30][31] have also found that MgSO 4 treatment had a neuroprotective effect in cerebral ischemia.…”

Section: Discussionsupporting

confidence: 93%

Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels after Cerebral Ischemia

Barışkaner¹,

Üstün

Ak³

et al. 2003

Pharmacology

View full text Add to dashboard Cite

show abstract

“…Magnesium, the second most common intracellular cation and the most abundant intracellular divalent cation (32)(33)(34), has been shown to be neuroprotective in several experimental models of ischemic and excitotoxic brain injury (20,21). However, other studies have failed to detect a neuroprotective effect of magnesium (22,23).…”

Section: Discussionmentioning

confidence: 99%

Hypoxia Induces an Increase in Intracellular Magnesium via Transient Receptor Potential Melastatin 7 (TRPM7) Channels in Rat Hippocampal Neurons in Vitro

Zhang¹,

Zhao²,

Zhao

et al. 2011

Journal of Biological Chemistry

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“…Magnesium sulfate has been shown to be neuroprotective in several experimental models of ischemic brain injury (Lampl et al, 2001;Izumi et al, 1991;Kaya et al, 2001;Miles et al, 2001;Yang et al, 2000;Şirin et al, 1998;Marinov et al, 1996;Chung et al, 2004). This effect may be due to the inhibition by Mg 2+ of the mitochondrial permeability transition (Zoratti and Szabò, 1995).…”

Section: Introductionmentioning

confidence: 99%

Ginkgo biloba Leaf Extract (EGb761) Combined with Neuroprotective Agents Reduces the Infarct Volumes of Gerbil Ischemic Brain

et al. 2006

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Ginkgo biloba exerts many pharmacological actions. It possesses antioxidant properties, the ability of neurotransmitter/receptor modulation and antiplatelet activation factor. This research is designed to investigate the neuroprotective effects of long-term treatment with EGb761 (a standard form of the extract of Ginkgo biloba leaf) in combination with MgSO(4), FK506, or MK-801 on the infarct volume of male gerbils' brain induced by unilateral middle cerebral artery occlusion (MCAO). Thirty-five gerbils fed a standard diet were intragastrically given water or EGb761 (100 mg/kg/day) for one week. Five randomized groups were established: control (n = 7), EGb761 (n = 8), EGb761 + MgSO(4) (n = 7), EGb761 + FK506 (n = 7), and EGb761 + MK-801 (n = 6). The three drug-combination groups were injected with MgSO(4) (90 mg/kg), FK506 (0.5 mg/kg), or MK-801 (1 mg/kg), respectively 30 min before MCAO. Gerbils were anesthetized and craniectomized to expose the right middle cerebral artery (MCA). The right MCA was constricted with an 8-0 suture to produce a permanent ligation for 24 hours. Postmortem infarct volumes were determined by quantitative image analysis of 2,3,5-triphenyltetrazolium chloride (TTC)-stained brain sections. Results showed that the total infarct volumes of the four treated groups either EGb761 alone or in combination with drugs were lower than the control group by 36.1% (EGb761 alone), 40.3% (EGb761 + MgSO(4)), 35.3% (EGb761 + FK506), and 56.4% (EGb761 + MK-801), respectively (p < 0.01). The main affected areas of the brain in the four treated groups were significantly focused between 4 and 6 mm from the frontal pole, when compared to the control group (p < 0.01). All animals in the five groups had infarctions in both cortex and subcortex. These results indicate that long-term pre-treatment of EGb761 administered either alone or in combination with drugs significantly effective neuroprotection on infarct volume in gerbil ischemic brains.

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Postischemic Intravenous Administration of Magnesium Sulfate Inhibits Hippocampal CA1 Neuronal Death after Transient Global Ischemia in Rats

Abstract: These results demonstrate a neuroprotective intravenous dose of MgSO(4), which is effective when administered before or late after ischemia, and a previously uncharacterized dose-response curve for MgSO(4).

Cited by 47 publications

References 30 publications

Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels after Cerebral Ischemia

Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels after Cerebral Ischemia

Hypoxia Induces an Increase in Intracellular Magnesium via Transient Receptor Potential Melastatin 7 (TRPM7) Channels in Rat Hippocampal Neurons in Vitro

Ginkgo biloba Leaf Extract (EGb761) Combined with Neuroprotective Agents Reduces the Infarct Volumes of Gerbil Ischemic Brain

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