Postirradiation Growth, Division, and Recovery in Bacteria

High-efficiency transformation of bacterial cells by electroporation

“…UV sensitivity in such cell lines is typically an order of magnitude greater than that of normal cells, as judged by the slope of the survival curves (1). It has been reported that growth at 43°C or growth on plates containing pantoyl lactone after irradiation can restore near normal survival in such cultures (2).…”

CHARACTERS OF NON-FILAMENTOUS DERIVATIVES OF <i>ESCHERICHIA</i> <i>COLI</i> STRAIN B_s-1 COMPARED WITH RELATED STRAINS OF <i>E. COLI</i> K12 AND B

“…This strain is, however, still filamentous (Fil+) in the sense that less filamentous (Fil-) strains can be derived from strain BS_1 as also reported by Witkin (1967) and that cells of strain BS_1 produce filaments after U V irradiation although their filaments are shorter than those found in UV-irradiated cells of strain B as also pointed out by Adler and Hardigree (1965) for the case of X-ray irradiation.…”

“…Adler and Hardigree (1965) have already reported that BS_1 shows shiftdown recovery after X-ray irradiation. Throughout this paper we regard strain BS_1 as a Fil+ strain.…”

“…It is not clear yet whether these three loci are identical or not. Hill and Feiner (1964) and Adler and Hardigree (1965) regarded strain BS_1 as a Filstrain.…”

NAD-dependent Recovery of Cell Division in UV-irradiatedEscherichia coliB and Lon-Mutant ofEscherichia coliK-12

“…1 ,2) Our previous studies have shown that phenethyl alcohol and butyl alcohol reverse this kind of UV-damage by suppressing the initiation of DNA-replication and possess division stimulating activities 3 ,4) which are similar to those found with pantoyl lactone. 5 ) Now we aimed to isolate active materials capable of repairing UV-damage from bacterial cells. Assay for the activity of divisionstimulating materials was carried out by burying the cell suspension of irradiated E. coli B in bouillon-agar plates with or without test materials which were passed through a 0.45 p membrane filter (Sartorius Co.) to remove contaminating cells and by counting the macrocolonies after 48-hr incubation at 37°C as previously described.…”