Postinternalization Inhibition of Adenovirus Gene Expression and Infectious Virus Production in Human T-Cell Lines

Phylogenetic Analysis and Structural Predictions of Human Adenovirus Penton Proteins as a Basis for Tissue-Specific Adenovirus Vector Design

“…A proof of principle that modification of the penton base is a feasible strategy in the retargeting of HAdV-C5 backbone vectors was the insertion of a hemagglutinin epitope (10). Thus, a retargeted vector with a species HAdV-C backbone, which provides features like the capability of causing persistent infections in immunocompetent patients by its early genes, may be achieved (27,30). Considering our modeling, swapping of penton base HVRs between HAdV types seems to be feasible, since the structures adjacent to the HVRs are very well conserved between the different HAdV species (Fig.…”

Neutralized Adenovirus-Immune Complexes Can Mediate Effective Gene Transfer via an Fc Receptor-Dependent Infection Pathway

“…ϩ CD8 ϩ CD3 ϩ T lymphocytes appear to be the major harbor for Ad in lymphoid tissue and support low levels of viral replication, although a small but detectable population of Ad was detected in a CD4 ϩ CD8 ϩ CD3 Ϫ population that included dendritic cells (16,43). Garnett et al (16) noted that the level of Ad DNA decreased with age in the study population, correlating with the age-related loss of Fc receptor expression on follicular dendritic cells (4).…”

Modeling Adenovirus Latency in Human Lymphocyte Cell Lines

“…As an initial step in developing a picture of adenovirus infections in lymphocytes, a number of groups have reported success at infecting human lymphocyte cell lines with species C adenoviruses, and most infections appeared to be nonlytic (5,17,19,20,33), at least over the short term. In this report we explore adenovirus species C infection of several T-and B-lymphocyte cell lines to determine the nature of the virus-host cell interaction over extended periods of time and to unravel the cellular mechanisms controlling the virus replicative cycle as well as changes in the host cell initiated by the virus.…”

“…Transduction of human lymphocyte cell lines with a CAR-expressing retrovirus was performed essentially as described before with a few modifications (19). In brief, Phoenix packaging cells were subcultured onto 100-mm culture dishes the day before transduction.…”

“…Infection of lymphocyte cell lines with adenovirus was performed as described previously (19) with minor modifications. Lymphocytes were collected and washed in serum-free (SF) RPMI medium.…”