Posthypoxic Reoxygenation-Induced Neurotoxicity Prevented by Free Radical Scavenger and NMDA/non-NMDA Antagonist in Tandem as Revealed by Dynamic Changes in Glucose Metabolism with Positron Autoradiography

Neuroprotective effect of chronic lithium treatment against hypoxia in specific brain regions with upregulation of cAMP response element binding protein and brain‐derived neurotrophic factor but not nerve growth factor: comparison with acute lithium treatment

“…Alamar Blue (AccuMed International Inc., Westklake, OH, USA) is an indicator of metabolic activity, and this dye is reduced by mitochondrial respiration (25). Previously we evaluated the brain slice neuronal viability after hypoxia loading using Alamar Blue (26). When CMRglc showed a significant decrease relative to control values, Alamar Blue reduction similarly showed corresponding significant changes, and when CMRglc did not show significant changes, Alamar Blue reduction did not show significant changes either.…”