Postharvest processing decreases the richness of bacterial taxa in the phyllosphere of broccoli

Kim, Min-Soo; Bae, Jin‐Woo; Park, E.-J.

doi:10.1111/jam.13759

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…All procedures for sample collection and preparation were described precisely [ 8 , 31 ]. Briefly, 66 florets of broccoli were collected from 22 farms distributed in four regions of Jeju Island (six farms in Daejeong [A], five farms in Hallim [B], five farms in Jocheon [C], and six farms in Seongsan [D]) during harvest season from November 2014 to February 2015 (Additional file 1 : Fig.…”

Section: Methodsmentioning

confidence: 99%

Composition and variability of core phyllosphere fungal mycobiota on field-grown broccoli

Kim

Park

2023

Environmental Microbiome

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Background Fresh vegetables harbor an assemblage of different microorganisms on their surfaces. The phyllosphere microbiota is important for maintaining plant health and managing crop quality before and after harvest. However, the diversity and ecology of fungal communities are largely unexplored in fresh vegetables. This study investigated the phyllosphere mycobiota of field-grown broccoli florets (n = 66) collected from 22 farms across four regions in Korea, using culturing, amplicon sequencing of the internal transcribed spacer region, and microbial network analysis. Results Microbial network analysis identified core genera (Purpureocillium, Filobasidium, Cystofilobasidium, Papiliotrema, Aureobasidium, and unclassified genera of Capnodiales) specific to the broccoli phyllosphere. The composition and network complexity of core and unique populations varied among farming regions, and was associated with local agro-meteorological conditions. The complexity of microbial associations was higher in mature communities than in immature communities, but complexity was lost upon development of plant pathogenic disease. Broccoli mycobiota were classified according to the dominance of Purpurecillium. While Purpurecillium-type microbiota were prevalent in normal samples, Filobasidium-type microbiota were frequently observed in immature, damaged, or postharvest samples. Conclusions Together, fungal communities were important components of phyllosphere microbiota on fresh vegetables, and have substantial potential for exploitation to enhance and stabilize plant health and growth.

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Section: Methodsmentioning

confidence: 99%

Composition and variability of core phyllosphere fungal mycobiota on field-grown broccoli

Kim

Park

2023

Environmental Microbiome

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Postharvest-induced microbiota remodeling increases fungal diversity in the phyllosphere mycobiota of broccoli florets

Kim

Park

2021

Postharvest Biology and Technology

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The food–gut axis: the phyllosphere virome of fresh vegetables is diverse, abundant, and transmitted to the human gut virome

Park,

Yun,

Cho

et al. 2024

Preprint

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Fresh vegetables harbor diverse microorganisms on leaf surfaces, yet their viral communities remain unexplored. We investigated the diversity and ecology of phyllosphere viromes of six leafy green vegetables using virus-like particle (VLP) enrichment and shotgun metagenome sequencing. On average, 9.2 × 107viruses were present per gram of leaf tissue. The majority (93.1 ± 6.2%) of these viruses were taxonomically unclassified. Virome compositions were distinct among vegetable types and exhibited temporal variations. Virulent phages with replication-enhancing auxiliary metabolic genes (AMGs) were more dominant than temperate phages with host fitness-benefiting AMGs. Analysis of 1,498 human fecal VLP metagenomes revealed that approximately 10% of vegetable viruses were present in the human gut virome, including viruses commonly observed in multiple studies. These gut-associated vegetable viruses were enriched with short-term vegetable intake, and depleted in individuals with metabolic and immunologic disorders. Overall, this study elucidates the ecological contribution of the fresh vegetable virome to human gut virome diversity.

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Postharvest processing decreases the richness of bacterial taxa in the phyllosphere of broccoli

Cited by 4 publications

References 40 publications

Composition and variability of core phyllosphere fungal mycobiota on field-grown broccoli

Composition and variability of core phyllosphere fungal mycobiota on field-grown broccoli

Postharvest-induced microbiota remodeling increases fungal diversity in the phyllosphere mycobiota of broccoli florets

The food–gut axis: the phyllosphere virome of fresh vegetables is diverse, abundant, and transmitted to the human gut virome

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