Postharvest Control of Gray Mold in Apples with Lyophilized Formulations of Cryptococcus laurentii: the Effect of Cold Stress in the Survival and Effectiveness of the Yeast

Navarta, Leonardo G.; Calvo, Juan; Posetto, Paola; Cerutti, Soledad; Raba, Julio; Benuzzi, Delia; Sanz, María I.

doi:10.1007/s11947-014-1303-0

Cited by 17 publications

(14 citation statements)

References 23 publications

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“…They found that C. infirmominiatum was sensitive and M. fructicola was relatively tolerant to oxidative stress. The antagonistic yeast C. laurentii has been widely studied and has shown excellent biocontrol efficacy against many postharvest diseases of apples, strawberries, mangoes, and sweet cherries ( Tian et al, 2004 ; Bautista-Rosales et al, 2014 ; Navarta et al, 2014 ; Zhang et al, 2015 ). A previous study has indicated that oxidative stress tolerance of an antagonistic yeast species is closely associated with its biocontrol performance in postharvest application ( Castoria et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit

Zhang

Chen

et al. 2017

Front. Microbiol.

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The limitations of chemical fungicides for the control of postharvest diseases have recently become more apparent. The utilization of antagonistic microorganisms is a promising alternative to that of fungicides to control postharvest decay. In previous studies, the antagonistic yeast Cryptococcus laurentii has shown excellent effects of biocontrol and great potential for practical application. Adverse conditions, such as oxidative stress, limit the practical application of antagonistic yeast. In this study, we investigated the oxidative stress tolerance of C. laurentii and the associated mechanisms. The results indicated that exogenous oxidative stress has a significant effect on the viability and biocontrol efficiency of C. laurentii. H2O2-induced oxidative stress led to the accumulation of reactive oxygen species. The results of flow cytometric analysis suggested that apoptosis is responsible for the reduced survival rate of C. laurentii under oxidative stress. Using tests of antioxidant activity, we found that C. laurentii could employ enzymatic systems to resist exogenous oxidative stress. The addition of exogenous glutathione, a non-enzymatic antioxidant, to the media can significantly enhance oxidative tolerance and biocontrol efficiency of C. laurentii.

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Section: Introductionmentioning

confidence: 99%

Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit

Zhang

Chen

et al. 2017

Front. Microbiol.

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“…; Navarta et al . ). Screening assays were designed based on previous studies, as illustrated in Fig.…”

Section: Discussionmentioning

confidence: 97%

“…; Navarta et al . ). The effect of protectants on the viability of agents varies depending on the microbial strain, and no information has been published on appropriate protectants for freeze‐dried Pi.…”

Section: Introductionmentioning

confidence: 97%

Optimization of a protective medium for freeze-dried Pichia membranifaciens and application of this biocontrol agent on citrus fruit

et al. 2016

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“…The use of biopesticides containing antagonistic microorganisms is a likely strategy, and the literature shows the successful use of bacteria and yeasts as biological control agents (BCAs) against pathogens [3,11,16,19,27]. A critical point in the application of biological control is the preparation of a formulation in which the active ingredient (microorganism) may be maintained stable, effective, and be safely handled.…”

Section: Introductionmentioning

confidence: 99%

Freeze-drying of a mixture of bacterium and yeast for application in postharvest control of pathogenic fungi

et al. 2020

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The use of formulations based on microorganisms for the control of phytopathogenic fungi increases continuously, due to the risk of pesticide residues in food and the negative impact on the environment. Herein we present the obtaining by lyophilization of a dry mixed formulation containing two biological control agents, the bacterium Kosakonia radicincitans and the yeast Cryptococcus laurentii for the biocontrol of Botrytis cinerea and Penicillium expansum on stored apple fruit. For the formulation is used a complex cryoprotectant, that consists of 10% skimmed nonfat milk, 0.5% yeast extract, and 1% glucose. Two pre-freezing temperatures and different concentrations of the cryoprotectant are tested. Survival and antagonistic capacity of the formulation are compared with the mix of the bacterium and the yeast separately lyophilized. Results show higher antagonism of the mixture against B. cinerea and P. expansum than each microorganism separately. Concerning survival, it decreases significantly for both microorganisms in comparison when they are lyophilized individually. The bacterium seems the most affected by joint lyophilization. In the lyophilized mixture, the viability of the microorganisms is 38.8% for K. radicincitans and 44.7% C. laurentii. While for microorganisms that are lyophilized individually, the viability is 92.5% for the bacterium and 81.9% for the yeast. The change in concentration of complex cryoprotectant improves survival. K. radicincitans reaches the highest survival value (53.9%), but the survival of C. laurentii is not altered. The antagonistic activity of the lyophilized mixture also improves. The incidence of disease for P. expansum decreases to 26% and for B. cinerea to 16%. The formulation developed could have practical advantages for its commercialization and application since the two biological control agents are present in a single product.

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Postharvest Control of Gray Mold in Apples with Lyophilized Formulations of Cryptococcus laurentii: the Effect of Cold Stress in the Survival and Effectiveness of the Yeast

Cited by 17 publications

References 23 publications

Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit

Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit

Optimization of a protective medium for freeze-dried Pichia membranifaciens and application of this biocontrol agent on citrus fruit

Freeze-drying of a mixture of bacterium and yeast for application in postharvest control of pathogenic fungi

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