Posterior Urethral Valves: The Experience of a Tunisian Department ofPediatric Surgery

Trabelsi, Fatma; Bouthour, H.; Bustame, Samer; Jabloun, A.; Abdallah, Rabiaa Ben; Kaabar, N

doi:10.4172/2572-4983.1000109

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…Most tissue engineering studies to date have constructed cell-seeded or acellular scaffolds that aim to reconstruct the epithelial and muscle layers, often ignoring the submucosa and the critical role of the surrounding corpus spongiosum in providing mechanical support and blood supply [10][11][12][13]. The corpus spongiosum is inadequate in hypospadias [14,15], so failure to replace this tissue may lead to long-term failure of urethral reconstruction [16]. Therefore, it is essential to develop tissue engineering strategies that involve all layers of the urethra.…”

Section: Introductionmentioning

confidence: 99%

Trilayer composite scaffold for urethral reconstruction: in vitro evaluation of mechanical, biological, and angiogenic properties

Abbas,

Parangusan,

Yalcin

et al. 2024

Biomed. Mater.

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Regeneration of damaged urethral tissue remains a major challenge in the field of lower urinary tract reconstruction. To address this issue, various synthetic and natural biodegradable biomaterials are currently being explored for the fabrication of scaffolds that promote urethral regeneration and healing. In this study, we present an approach to fabricate a trilayer hybrid scaffold comprising a central layer of poly(lactic acid) (PLA) between two layers of chitosan. The chitosan/PLA/chitosan (CPC) scaffolds were fabricated by a sequential electrospinning process and their properties were evaluated for their suitability for urethral tissue engineering. The physical and biological properties of the CPC scaffolds were evaluated in comparison to electrospun PLA scaffolds and acellular dermis (Alloderm) as controls for a synthetic and a natural scaffold, respectively. Compared to the controls, the CPC scaffolds exhibited higher elastic modulus and ultimate tensile strength, while maintaining extensibility and suture retention strength appropriate for clinical use. The CPC scaffolds displayed significant hydrophilicity, which was associated with a higher water absorption capacity of the chitosan nanofibres. The degradation products of the CPC scaffolds did not exhibit cytotoxicity and promoted wound closure by fibroblasts in vitro. In addition, CPC scaffolds showed increased growth of smooth muscle cells, an essential component for functional regeneration of urethral tissue. Furthermore, in a chicken embryo-based assay, CPC scaffolds demonstrated significantly higher angiogenic potential, indicating their ability to promote vascularisation, a crucial aspect for successful urethral reconstruction. Overall, these results suggest that CPC hybrid scaffolds containing both natural and synthetic components offer significant advantages over conventional acellular or synthetic materials alone. CPC scaffolds show promise as potential candidates for further research into the reconstruction of the urethra in vivo.

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