Postembryonic development of the posterior lateral line in the zebrafish

Nuñez, Viviana A; Sarrazin, Andres F.; Cubedo, Nicolas; Allende, Miguel L.; Dambly‐Chaudière, Christine; Ghysen, Alain

doi:10.1111/j.1525-142x.2009.00346.x

Cited by 66 publications

(75 citation statements)

References 28 publications

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“…For fate-mapping experiments, Kaede photoconversion was performed as described previously (Nuñez et al, 2009) using a 0.05 mm pinhole positioned as a field diaphragm in the excitation path of an Axioplan microscope (Zeiss) and a 20ϫ water-immersion objective, achieving an actual diameter of illumination of 25 m. For fate mapping at 17.5 hpf, we used a pinhole of 0.16 mm and an eyepiece reticle with a square lattice to position the beam relative to the outline of the otic vesicle. For synapse conversion, we also used a 0.16 mm pinhole and delivered two pulses of 30 s each, separated by 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…B, A case in which LI neuromasts, but not LII, are completely absent. LI and LII neuromasts can be distinguished based on the central, unlabeled region that is oriented along the anteroposterior axis in LI neuromasts and along the dorsoventral axis in LII (Nuñez et al, 2009). C, A case in which LII neuromasts, but not LI, are absent.…”

Section: Origin Of the Primary And Secondary Placodesmentioning

confidence: 99%

“…One migrates along the horizontal myoseptum, much as primI did, and has been called primII; the other one migrates along the dorsal midline and has been called primD (for review, see Ghysen and Dambly-Chaudière, 2007). primII and primD deposit seven to eight and five neuromasts, respectively, as well as a trail of interneuromast cells that proliferate later during larval development and form two new lines of neuromasts (Nuñez et al, 2009). primII and primD are therefore at the origin of three of the four anteroposterior lines of neuromasts present in juvenile and adult zebrafish, yet their origin has never been investigated.…”

Section: Introductionmentioning

confidence: 99%

“…By 48 hpf, the embryonic pattern is complete. In addition to the neuromasts, primI deposits a trail of interneuromast cells that will later proliferate and form intercalary neuromasts during larval development (Grant et al, 2005;LopezSchier and Hudspeth, 2005;Nuñez et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Sarrazin¹,

Nuñez²,

Sapède³

et al. 2010

Journal of Neuroscience

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The lateral line system of teleosts has recently become a model system to study patterning and morphogenesis. However, its embryonic origins are still not well understood. In zebrafish, the posterior lateral line (PLL) system is formed in two waves, one that generates the embryonic line of seven to eight neuromasts and 20 afferent neurons and a second one that generates three additional lines during larval development. The embryonic line originates from a postotic placode that produces both a migrating sensory primordium and afferent neurons. Nothing is known about the origin and innervation of the larval lines. Here we show that a "secondary" placode can be detected at 24 h postfertilization (hpf), shortly after the primary placode has given rise to the embryonic primordium and ganglion. The secondary placode generates two additional sensory primordia, primD and primII, as well as afferent neurons. The primary and secondary placodes require retinoic acid signaling at the same stage of late gastrulation, suggesting that they share a common origin. Neither primary nor secondary neurons show intrinsic specificity for neuromasts derived from their own placode, but the sequence of neuromast deposition ensures that neuromasts are primarily innervated by neurons derived from the cognate placode. The delayed formation of secondary afferent neurons accounts for the capability of the fish to form a new PLL ganglion after ablation of the embryonic ganglion at 24 hpf.

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Section: Methodsmentioning

confidence: 99%

Section: Origin Of the Primary And Secondary Placodesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Sarrazin¹,

Nuñez²,

Sapède³

et al. 2010

Journal of Neuroscience

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“…5 for zebrafish) lead to the juvenile stage, around 1 month post fertilization (mpf). At this stage, about 50 PLL neuromasts are aligned as four antero-posterior lines (6) (Fig. S1A).…”

mentioning

confidence: 99%

Innervation is required for sense organ development in the lateral line system of adult zebrafish

Wada

Dambly‐Chaudière

Kawakami

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Superficial mechanosensory organs (neuromasts) distributed over the head and body of fishes and amphibians form the "lateral line" system. During zebrafish adulthood, each neuromast of the body (posterior lateral line system, or PLL) produces "accessory" neuromasts that remain tightly clustered, thereby increasing the total number of PLL neuromasts by a factor of more than 10. This expansion is achieved by a budding process and is accompanied by branches of the afferent nerve that innervates the founder neuromast. Here we show that innervation is essential for the budding process, in complete contrast with the development of the embryonic PLL, where innervation is entirely dispensable. To obtain insight into the molecular mechanisms that underlie the budding process, we focused on the terminal system that develops at the posterior tip of the body and on the caudal fin. In this subset of PLL neuromasts, bud neuromasts form in a reproducible sequence over a few days, much faster than for other PLL neuromasts. We show that wingless/int (Wnt) signaling takes place during, and is required for, the budding process. We also show that the Wnt activator R-spondin is expressed by the axons that innervate budding neuromasts. We propose that the axon triggers Wnt signaling, which itself is involved in the proliferative phase that leads to bud formation. Finally, we show that innervation is required not only for budding, but also for long-term maintenance of all PLL neuromasts.Lgr | post-embryonic development | Thunnus thynnus | Danio rerio

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Ear and Lateral Line of Vertebrates: Organisation and Development

Harding

McCarroll

McGraw

et al. 2013

Encyclopedia of Life Sciences

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In vertebrates, perception of movement and sound is accomplished by the lateral line and inner ear sensory systems. These systems sense reverberations, movement and acceleration by transducing mechanical stimuli from the environment into electrical signals by means of mechanosensory hair cells. Vestibular and auditory hair cells have associated sensory neurons that transmit these signals from the periphery to the central nervous system. During development, cranial sensory systems arise from an initially homogeneous population of cells that ultimately give rise to discrete sensory structures. Although the demands for auditory and vestibular sensation differ between species and environments, vertebrates use common cell types, genetic programmes and molecules to achieve the development of these mechanosensory organs. In this article, the structure and function of the mechanosensory hair cells, lateral line and inner ear and how these systems develop across species are discussed, and as well as the innervation of these systems. Key Concepts: The vertebrate inner ear is composed of both auditory and vestibular components. Both the lateral line and the inner ear are derived from embryonic structures known as cranial placodes. All cranial placodes originate from a homogenous group of cells known as the preplacodal ectoderm. Hair cells are structurally and functionally similar in both auditory and lateral line systems. The adult lateral line mediates sensation of movement in the aquatic environment of fishes and frogs. Embryonic posterior lateral line development is accomplished by the pre‐patterned posterior lateral line primordium. The lateral line is an experimentally accessible model for studying mechanosensory system development and biology.

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Postembryonic development of the posterior lateral line in the zebrafish

Cited by 66 publications

References 28 publications

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Innervation is required for sense organ development in the lateral line system of adult zebrafish

Ear and Lateral Line of Vertebrates: Organisation and Development

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