Post translational modifications of milk proteins in geographically diverse goat breeds

Rout, P. K.; Verma, Mahima

doi:10.1038/s41598-021-85094-9

Cited by 25 publications

(20 citation statements)

References 67 publications

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“…Peaks at m / z : 14,386, 14,496, and 14,599 correspond to other protein forms. Molar mass values of proteins will be differentiated by many factors (protein origin, post-translational modifications, and calcium content). ,, The protein can be glycosylated at the asparagine moiety. , Consequently, many isoforms can be characterized. , The spectrum also shows a peak from the dimeric form occurring at m / z 28,326 and trimeric form (at m / z 42,495) and a low intensity peak from the doubly ionized form of the pseudo-molecular ion at an m / z of 7081. The choice of the matrix is crucial for observation and the stability of protein oligomers and isoforms under measurement conditions.…”

Section: Resultsmentioning

confidence: 99%

Functionalization of Alpha-Lactalbumin by Zinc Ions

et al. 2022

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Alpha-lactalbumin (α-LA) and binding of zinc cations to protein were studied. Molecular characteristics of protein was determined by MALDI-TOF/MS and electrophoresis SDS-PAGE, and also, for complexes, it was determined by spectroscopic techniques (ATR-FT-IR and Raman) and microscopic techniques (SEM along with an EDX detector and also TEM). The pH dependence of zeta potential of α-LA was determined in saline solution. The zinc binding to the protein mechanism was investigated; zinc binding to protein kinetics, the molecular modeling by the DFT method, and electron microscopy (SEM and TEM) for microstructure observation were performed. The experiments performed indicate a quick binding process (equilibrium takes place after 2 min of incubation) which occurs onto the surface of α-LA. Zinc cations change the conformation of the protein and create spherical particles from the morphological point of view. DFT studies indicate the participation of acidic functional groups of the protein (aspartic acid and glutamic acid residues), and these have a decisive influence on the interaction with zinc cations. Application studies of general toxicity and cytotoxicity and bioavailability were conducted.

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Section: Resultsmentioning

confidence: 99%

Functionalization of Alpha-Lactalbumin by Zinc Ions

et al. 2022

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“…The most abundant milk proteins are: αs1(CSN1S1), αs2 (CSN1S2), β (CSN2) and κ-casein (CSN3), β-lactoglobulin (BLG), and α-lactalbumin (LALBA) and they represent 95% of the total protein content in ruminant milk [89]. These proteins Goat Science -Environment, Health and Economy are encoded on chromosome 6 in a segment of 250 kbps [90], have different posttranslation modification [91], and their milk concentration changes according to the gene expression of these casein genes [92].…”

Section: αS1 αS2 β and κ-Casein Polymorphismmentioning

confidence: 99%

“…These casein genes have a polymorphism within the same breed [93] and among diverse breeds [27,90], and this biodiversity might impact the goat milk quality and milk properties in term of their role with the immune system, nutritional quality, and as raw material to produce other products derived from milk [91].…”

Section: αS1 αS2 β and κ-Casein Polymorphismmentioning

confidence: 99%

Goat Type Selection and Molecular Markers; a Solution for Milk Production in Recently Desertified Zones

Strahsburger¹,

Scopinich-Cisternas²

2023

Goat Science - Environment, Health and Economy

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Goat farming has been severely affected by Desertification, limiting their water and food resources and inducing physiological heat stress that reduces the doe milk yield. Does well adapted to heat stress would be a possible solution, but creole or indigenous goats from desert or arid areas produce between 0.5 to 1.5 L of milk per day, which is lower than the 3 L of milk per day produced by dairy goats like the Saanen breed. Nevertheless, in this chapter, we will discuss the disadvantages of introducing common dairy goats in dry places. Instead, we propose the introduction of desert goats from the Middle East or India, because they produce high-quality milk with low feed intake, making a profitable goat farming activity, and an opportunity to include crossbreeding strategies to improve the herd milk yield. Creole goats, on other hand, has been an underestimated livestock animal with a rich and unveil genetic patrimony that migth improve the herd milk yield. The effect of improved diets and extensive husbandry conditions remains unexplored in desert creole goats, and the use of advanced knowledge in goat genomics, genetic expression, and a wide variety of molecular markers can improve the studies on creole goats for crossbreeding strategies identifying the best traits involved in high-quality milk production and adaptation to dry environments. In this way, the synergy between goat type selection and molecular markers should boost goat farming in recently new desert or arid zones, counteracting the detrimental effects produced by the desertification.

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“…Advances in proteomics have improved the identification of these compounds. Through an approach using the connection of several mass analyzers (MS/MS), it has been possible to decipher proteins of the membrane of milk fat globules of different species [68]. Pisanu et al [69] managed to identify 140 proteins belonging to this membrane in sheep's milk using LC tandem mass spectrometry (LC-MS/MS).…”

Section: Advances In Proteomics In the Characterization Of Milk And D...mentioning

confidence: 99%

Proteomic Advances in Milk and Dairy Products

et al. 2021

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Proteomics is a new area of study that in recent decades has provided great advances in the field of medicine. However, its enormous potential for the study of proteomes makes it also applicable to other areas of science. Milk is a highly heterogeneous and complex fluid, where there are numerous genetic variants and isoforms with post-translational modifications (PTMs). Due to the vast number of proteins and peptides existing in its matrix, proteomics is presented as a powerful tool for the characterization of milk samples and their products. The technology developed to date for the separation and characterization of the milk proteome, such as two-dimensional gel electrophoresis (2DE) technology and especially mass spectrometry (MS) have allowed an exhaustive characterization of the proteins and peptides present in milk and dairy products with enormous applications in the industry for the control of fundamental parameters, such as microbiological safety, the guarantee of authenticity, or the control of the transformations carried out, aimed to increase the quality of the final product.

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Post translational modifications of milk proteins in geographically diverse goat breeds

Cited by 25 publications

References 67 publications

Functionalization of Alpha-Lactalbumin by Zinc Ions

Functionalization of Alpha-Lactalbumin by Zinc Ions

Goat Type Selection and Molecular Markers; a Solution for Milk Production in Recently Desertified Zones

Proteomic Advances in Milk and Dairy Products

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