Post-Translational Modifications of Exosomal Proteins

Moreno-Gonzalo, Olga; Villarroya-Beltrí, Carolina

doi:10.3389/fimmu.2014.00383

Cited by 97 publications

(91 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, ubiquitin and UBLs are not the only PTMs detected in EVs [73,107]. For example, many phosphorylated proteins appear in exosomes, including the calcium-dependent phospholipid-binding protein Annexin A2, which is enriched in cholesterol-rich lipid raft micro-domains of exosome membranes [101,108,109].…”

Section: Other Ptms In Evsmentioning

confidence: 99%

“…Specific antibodies for ubiquitin and poly-ubiquitin chains allowed to identify mono-, oligoand poly-ubiquitinated proteins in exosomes [57]. Various studies and proteomic analysis identified specific ubiquitinated proteins (reviewed in [73]) ( Table 2 and Fig. 1).…”

Section: Ubiquitinated Proteins In Evsmentioning

confidence: 99%

“…Besides, protein glycosylation has improved the efficacy of therapeutic proteins [143]. Thus, addition of glycosylating motifs to the protein of interest could also mediate its incorporation to exosomes, although protein glycosylation is still technically complex for industrial production [73,107] (Fig. 3).…”

Section: Disease Treatmentmentioning

confidence: 99%

“…1). Moreover, several studies have revealed the presence of glycosylated proteins in EVs (reviewed in [73,107]). Notably, EVs contain mainly mannose or sialic acid enriched-glycans and N-linked glycoproteins.…”

Section: Other Ptms In Evsmentioning

confidence: 99%

See 3 more Smart Citations

Post-translational add-ons mark the path in exosomal protein sorting

Moreno-Gonzalo

Fernández-Delgado

Sánchez‐Madrid

2017

Cell. Mol. Life Sci.

Self Cite

View full text Add to dashboard Cite

Section: Other Ptms In Evsmentioning

confidence: 99%

Section: Ubiquitinated Proteins In Evsmentioning

confidence: 99%

Section: Disease Treatmentmentioning

confidence: 99%

Section: Other Ptms In Evsmentioning

confidence: 99%

See 2 more Smart Citations

Post-translational add-ons mark the path in exosomal protein sorting

Moreno-Gonzalo

Fernández-Delgado

Sánchez‐Madrid

2017

Cell. Mol. Life Sci.

Self Cite

View full text Add to dashboard Cite

“…In vivo composition of exosomes seems not to be formed in a random process but by a regulated selection of specific components. 71 Several most recent studies on therapeutic potential of extracellular vesicles of various origin were presented by Merino et al 72 On the other hand, it should be stressed that approaches to target exosomes mediating pathological intercellular communication during the disease course may also show high therapeutic potential 73 ( FIgure 3A).…”

mentioning

confidence: 99%

Exosomes as mediators of intercellular communication: clinical implications

Nazimek¹,

Bryniarski²,

Santocki³

et al. 2015

Polish Archives of Internal Medicine

View full text Add to dashboard Cite

mainly through: 1) simple membrane-crossing diffusion (eg, transport of steroids); 2) active transport via membrane ion-channels, pumps, or transporting proteins; 3) the formation of synapses including immunological and nerve synapses; and 4) the exchange of cell membrane fragments termed trogocytosis.1 Furthermore, cells communicate in an auto-, para-, and endocrine manner by sensing circulating endogenous bioactive compounds including: 1) proteins (eg, hormones, cytokines), lipoproteins, lipids, and steroids; 2) simpler compounds such as eicosanoids, monoamines (eg, neurotransmitters), endorphins, or cannabinoids; and, finally, 4) extracellular microRNA (miRNA) molecules associated with protein chaperones. The majority of these signaling compounds were found in the content of extracellular vesicles, mainly exosomes. It should be stressed that the interaction of extracellular vesicles with targeted cells can produce varied biological effects mainly resulting from direct exosome-cell stimulation and action of transferred exosome cargo. 2 Exosomes release their content into an acceptorIntroduction Studies of the last decade uncovered the special role of extracellular vesicles, especially exosomes, in local and systemic cell-to-cell communication. Moreover, exosomes were shown to carry various bioactive compounds including proteins, lipids, and diverse RNA molecules. At present, particular emphasis is placed on the extremely high potential of exosomes as prognostic and diagnostic markers as well as therapeutic nanocarriers, especially after demonstration that their cargo and targeting specificity could be manipulated.The current review briefly summarizes the role of exosomes in intercellular signaling pathways orchestrating varied biological mechanisms, with a special focus on the use of exosomes in the diagnosis and treatment of various inflammatory, cardiovascular, metabolic, and neurodegenerative disorders as well as cancer. Modes of cell-to-cell communication AbstrActCells of multicellular organisms exchange informative signals by diverse mechanisms. Recent findings uncovered the special role of extracellular vesicles, especially exosomes, in intercellular communication. Exosomes, present in all tested human bodily fluids, carry various functional compounds including proteins, lipids, and diverse RNA molecules. The composition of exosome cargo in vivo is likely formed by a regulated selection of specific components and can express the current status of the exosome--secreting cell. Therefore, particular emphasis is now placed on the extremely high potential of exosomes as essentially noninvasive prognostic and diagnostic biomarkers, but also as therapeutic nanocarriers, especially after the discovery that their cargo as well as cell-targeting specificity could be shaped in vitro. In addition, targeting the exosomes mediating pathological intercellular communication may also express high therapeutic potential. Hence, numerous studies are conducted to explore the profile and function of exosomes and their cargo ...

show abstract

Exosomes as Potential Functional Nanomaterials for Tissue Engineering

Yao

Jiang

Song

et al. 2022

Adv Healthcare Materials

View full text Add to dashboard Cite

Exosomes are cell‐derived extracellular vesicles of 40–160 nm diameter, which carry numerous biomolecules and transmit information between cells. They are used as functional nanomaterials with great potential in biomedical areas, such as active agents and delivery systems for advanced drug delivery and disease therapy. In recent years, potential applications of exosomes in tissue engineering have attracted significant attention, and some critical progress has been made. This review gives a complete picture of exosomes and their applications in the regeneration of various tissues, such as the central nervous systems, kidney, bone, cartilage, heart, and endodontium. Approaches employed for modifying exosomes to equip them with excellent targeting capacity are summarized. Furthermore, current concerns and future outlook of exosomes in tissue engineering are discussed.

show abstract

Post-Translational Modifications of Exosomal Proteins

Cited by 97 publications

References 57 publications

Post-translational add-ons mark the path in exosomal protein sorting

Post-translational add-ons mark the path in exosomal protein sorting

Exosomes as mediators of intercellular communication: clinical implications

Exosomes as Potential Functional Nanomaterials for Tissue Engineering

Contact Info

Product

Resources

About