Post-translational modification of plant-made foreign proteins; glycosylation and beyond

Webster, Diane; Thomas, Merlin C.

doi:10.1016/j.biotechadv.2011.07.015

Cited by 74 publications

(49 citation statements)

References 108 publications

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“…It also alters the essential biological functions, such as immunogenicity, catalytic activity, clearance, ligand-receptor interactions, etc. (Webster and Thomas, 2012). Two major types of protein glycosylation have been discovered in eukaryotes, O-linked and N-linked glycosylation.…”

Section: Resultsmentioning

confidence: 99%

Correlations between predicted protein disorder and post-translational modifications in plants

et al. 2014

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Motivation: Protein structural research in plants lags behind that in animal and bacterial species. This lag concerns both the structural analysis of individual proteins and the proteome-wide characterization of structure-related properties. Until now, no systematic study concerning the relationships between protein disorder and multiple post-translational modifications (PTMs) in plants has been presented.Results: In this work, we calculated the global degree of intrinsic disorder in the complete proteomes of eight typical monocotyledonous and dicotyledonous plant species. We further predicted multiple sites for phosphorylation, glycosylation, acetylation and methylation and examined the correlations of protein disorder with the presence of the predicted PTM sites. It was found that phosphorylation, acetylation and O-glycosylation displayed a clear preference for occurrence in disordered regions of plant proteins. In contrast, methylation tended to avoid disordered sequence, whereas N-glycosylation did not show a universal structural preference in monocotyledonous and dicotyledonous plants. In addition, the analysis performed revealed significant differences between the integral characteristics of monocot and dicot proteomes. They included elevated disorder degree, increased rate of O-glycosylation and R-methylation, decreased rate of N-glycosylation, K-acetylation and K-methylation in monocotyledonous plant species, as compared with dicotyledonous species. Altogether, our study provides the most compelling evidence so far for the connection between protein disorder and multiple PTMs in plants.Contact: tokmak@phoenix.kobe-u.ac.jp or tetsuya.sakurai@riken.jpSupplementary information: Supplementary data are available at Bioinformatics online.

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Section: Resultsmentioning

confidence: 99%

Correlations between predicted protein disorder and post-translational modifications in plants

et al. 2014

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“…Plant-derived IL6 might be differentially glycosylated in a similar manner to the native protein, since the potential N - and O -glycosylation sites of IL6, as described by Orita et al . [74], were found to be utilized in plants and humans [75]–[77]. Though, despite conserved recognition sites, the structure of the glycan moieties differs between humans and plants [75]–[78].…”

Section: Discussionmentioning

confidence: 99%

High-Level Transient Expression of ER-Targeted Human Interleukin 6 in Nicotiana benthamiana

et al. 2012

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Tobacco plants can be used to express recombinant proteins that cannot be produced in a soluble and active form using traditional platforms such as Escherichia coli. We therefore expressed the human glycoprotein interleukin 6 (IL6) in two commercial tobacco cultivars (Nicotiana tabacum cv. Virginia and cv. Geudertheimer) as well as the model host N. benthamiana to compare different transformation strategies (stable vs. transient expression) and subcellular targeting (apoplast, endoplasmic reticulum (ER) and vacuole). In T0 transgenic plants, the highest expression levels were achieved by ER targeting but the overall yields of IL6 were still low in the leaves (0.005% TSP in the ER, 0.0008% in the vacuole and 0.0005% in the apoplast). The apoplast variant accumulated to similar levels in leaves and seeds, whereas the ER-targeted variant was 1.2-fold more abundant in seeds and the vacuolar variant was 6-fold more abundant in seeds. The yields improved in subsequent generations, with the best-performing T2 plants producing the ER-targeted IL6 at 0.14% TSP in both leaves and seeds. Transient expression of ER-targeted IL6 in leaves using the MagnICON system resulted in yields of up to 7% TSP in N. benthamiana, but only 1% in N. tabacum cv. Virginia and 0.5% in cv. Geudertheimer. Although the commercial tobacco cultivars produced up to threefold more biomass than N. benthamiana, this was not enough to compensate for the lower overall yields. The recombinant IL6 produced by transient and stable expression in plants was biologically active and presented as two alternative bands matching the corresponding native protein.

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“…44-46 Yeast expression systems work similarly to bacterial systems and can also be scaled up using fermentation technology. Some common yeast species that have been developed for VLP production include Saccharomyces cerevisiae and Pichia pastoris , 47 and VLPs that have successfully been produced in yeast include Qβ, 47 CCMV, 48 and HPV, 49 which incidentally is how Merck produces the vaccine Gardasil.…”

Section: Viruses In a Materials Worldmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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Post-translational modification of plant-made foreign proteins; glycosylation and beyond

Cited by 74 publications

References 108 publications

Correlations between predicted protein disorder and post-translational modifications in plants

Correlations between predicted protein disorder and post-translational modifications in plants

High-Level Transient Expression of ER-Targeted Human Interleukin 6 in Nicotiana benthamiana

Design of virus-based nanomaterials for medicine, biotechnology, and energy

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