Post-transcriptional Regulation of FLOWERING LOCUS T Modulates Heat-Dependent Source-Sink Development in Potato

Lehretz, Günter G.; Sonnewald, Sophia; Hornyik, Csaba; Corral, José M. Miguel del; Sonnewald, Uwe

doi:10.1016/j.cub.2019.04.027

Cited by 66 publications

(68 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AcFT1 promotes bulb formation, while AcFT4 inhibits bulb production in onions (Lee et al, 2013). Tuberization is regulated by the FT homolog StSP6A through modulation of source-sink in potatoes (Abelenda et al, 2019;Lehretz et al, 2019). Our transgenic plants were thinner than WT, which was caused by overexpression of PbFT.…”

Section: Expression and Function Of Pbftmentioning

confidence: 81%

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

Zhao¹,

Wei²,

Pang³

et al. 2020

PeerJ

View full text Add to dashboard Cite

Although Phosphatidylethanolamine-binding protein (PEBP) genes have been identified in several plants, little is known about PEBP genes in pears. In this study, a total of 24 PEBP genes were identified, in which 10, 5 and 9 were from Pyrus bretschneideri genome, Pyrus communis genome and Pyrus betuleafolia genome, respectively. Subsequently, gene structure, phylogenetic relationship, chromosomal localization, promoter regions, collinearity and expression were determined with these PEBP genes. It was found that only PbFT from PEBP genes of P. bretschneideri was relatively highly expressed in leaves during flower bud differentiation. Whereas, expression patterns of TFL1 homologues, gene23124 and gene16540, were different from PbFT in buds. The expression pattern and the treatment of reduction day-length indicated that the expression of PbFT in leaves were regulated by day-length and circadian clock. Additionally, the phenotype of transgenic Arabidopsis suggested that PbFT played a role in not only promoting flower bud differentiation, but also regulating the balance between vegetative and reproductive growth. These results may provide important information for further understanding of the evolution and function of PEBP genes in pears.

show abstract

Section: Expression and Function Of Pbftmentioning

confidence: 81%

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

Zhao¹,

Wei²,

Pang³

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…Figure 5) and therefore potentially constitute additional TAC targets. Of these, StMADS17 was previously described to be strongly upregulated in stolons (Gao et al, 2018) and shown to upregulated in the strongly induced to tuberise transgenic SES lines described by Lehretz et al (2019). StMADS17 has homology to SEPALLATA 4 (SEP 4) from Arabidopsis, with a reported role in the regulation of floral meristem development (Ditta et al, 2004).…”

Section: Impact Of Stcen On Stsp6a Gene Expressionmentioning

confidence: 99%

TERMINAL FLOWER‐1/CENTRORADIALIS inhibits tuberisation via protein interaction with the tuberigen activation complex

et al. 2020

View full text Add to dashboard Cite

SUMMARY Potato tuber formation is a secondary developmental programme by which cells in the subapical stolon region divide and radially expand to further differentiate into starch‐accumulating parenchyma. Although some details of the molecular pathway that signals tuberisation are known, important gaps in our knowledge persist. Here, the role of a member of the TERMINAL FLOWER 1/CENTRORADIALIS gene family (termed StCEN) in the negative control of tuberisation is demonstrated for what is thought to be the first time. It is shown that reduced expression of StCEN accelerates tuber formation whereas transgenic lines overexpressing this gene display delayed tuberisation and reduced tuber yield. Protein–protein interaction studies (yeast two‐hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components of the recently described tuberigen activation complex. Using transient transactivation assays, we show that the StSP6A tuberisation signal is an activation target of the tuberigen activation complex, and that co‐expression of StCEN blocks activation of the StSP6A gene by StFD‐Like‐1. Transcriptomic analysis of transgenic lines misexpressing StCEN identifies early transcriptional events in tuber formation. These results demonstrate that StCEN suppresses tuberisation by directly antagonising the function of StSP6A in stolons, identifying StCEN as a breeding marker to improve tuber initiation and yield through the selection of genotypes with reduced StCEN expression.

show abstract

“…Moreover, excessive temperatures have a detrimental effect on photosynthetic performance including severe inhibition of CO 2 fixation and chlorophyll loss in sensitive species (Reynolds et al, 1990). Recent work has demonstrated the mechanisms by which tuberization signaling and initiation is impaired at elevated temperature (Lehretz et al, 2019;Morris et al, 2019). However, there is wide variation for heat stress tolerance across potato germplasm that could be exploited to ensure the sustainability of yield in warmer climates (Levy and Veilleux, 2007;Trapero-Mozos et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Physiological, Biochemical, and Transcriptional Responses to Single and Combined Abiotic Stress in Stress-Tolerant and Stress-Sensitive Potato Genotypes

et al. 2020

View full text Add to dashboard Cite

show abstract

Post-transcriptional Regulation of FLOWERING LOCUS T Modulates Heat-Dependent Source-Sink Development in Potato

Abstract: Highlights d Tuberization is developmentally and temperature dependent regulated via SP6A d SP6A expression is repressed under heat by a small RNA d Abolishing miRNA activity facilitates tuberization, even under strict heat conditions

Cited by 66 publications

References 63 publications

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

TERMINAL FLOWER‐1/CENTRORADIALIS inhibits tuberisation via protein interaction with the tuberigen activation complex

Physiological, Biochemical, and Transcriptional Responses to Single and Combined Abiotic Stress in Stress-Tolerant and Stress-Sensitive Potato Genotypes

Contact Info

Product

Resources

About