Post-transcriptional regulation of c-fms proto-oncogene expression by dexamethasone and of CSF-1 in human breast carcinomas in vitro

Chambers, Setsuko K.; Wang, Yixun; Gilmore-Hebert, Maureen; Kacinski, Barry M.

doi:10.1016/0039-128x(94)90069-8

Cited by 19 publications

(25 citation statements)

References 45 publications

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“…Figure 5b confirms the overexpression of HuR protein, and shows the increase in c-fms protein by HuR, under dex-stimulated conditions. Under resting (no dex) conditions, bands on western blot were barely detectable (not shown), in line with other reports (Kacinski et al, 1991;Chambers et al, 1994;Sapi et al, 1995). These findings were duplicated in another breast-cancer cell line, SKBR3 (Figures 5d-f).…”

Section: Mutations In the 69-nt Element Ofsupporting

confidence: 91%

“…When stimulated with dex, c-fms RNA was >200-fold under control conditions compared with HuR shRNA (Figure 6a), accounting for more than the known 50-fold stimulation of c-fms RNA and protein by dex in breast-cancer cells (Kacinski et al, 1991;Chambers et al, 1994;Sapi et al, 1995). HuR shRNA resulted in undetectable c-fms protein levels (Figure 6b).…”

Section: Discussionmentioning

confidence: 95%

“…We reported that physiological concentrations of glucocorticoids upregulate c-fms expression by up to 50-fold in breast cancer cell lines (Kacinski et al, 1991;Chambers et al, 1994;Sapi et al, 1995). These results were confirmed by using a primary organ culture system of fresh breast-carcinoma specimens (Kacinski et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Control of c-fms expression is related to both transcriptional and post-transcriptional events (Weber et al, 1989;Chambers et al, 1993Chambers et al, , 1994Chambers et al, , 2004Sapi et al, 1995). In monocytes, we have found that dexamethasone (dex) alters c-fms mRNA transcript half-life (Chambers et al, 1993); in breast cancer cells, both mechanisms regulate c-fms expression (Chambers et al, 1994;Sapi et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…In monocytes, we have found that dexamethasone (dex) alters c-fms mRNA transcript half-life (Chambers et al, 1993); in breast cancer cells, both mechanisms regulate c-fms expression (Chambers et al, 1994;Sapi et al, 1995). Post-transcriptional events are frequently modulated by protein binding to 3 0 UTR mRNA; yet, the existence and identity of the c-fms RNA regulatory protein/s remain unknown.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer

Woo

Zhou

et al. 2009

Oncogene

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Section: Mutations In the 69-nt Element Ofsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer

Woo

Zhou

et al. 2009

Oncogene

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Interaction of AU-rich sequence binding proteins with actin: Possible involvement of the actin cytoskeleton in lymphokine mRNA turnover

1997

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In the current study, we report that cytochalasin-induced disruption of microfilaments stabilizes lymphokine mRNAs in activated human peripheral blood lymphocytes. Parallel with this, a dose-and time-dependent increase in AU-rich sequence binding protein (AUPB) activities is apparent in the nonionic detergentresistant fractions of these cells, suggesting that cytochalasin-induced modulation of lymphokine mRNA stability might be mediated through cytoplasmic AUBPs. We provide evidence that some of the AUBPs can be immunoprecipitated with anti-actin antibodies, implicating the potential of these proteins to associate with the actin-based cytoskeleton in vivo. Moreover, disruption of the microfilament network by cytochalasins produces increased immunoprecipitable actin-AUBP complexes in the detergent-resistant cytoplasmic subfractions of lymphocytes. We show that cytochalasin-induced changes in AUBP activities are parallel with their higher binding affinity to RNA containing AU-rich instability sequence element as judged by in vitro competition and in vivo ultraviolet-crosslinking analysis. Correlation of these findings with changes in mRNA stability indicates that the actin cytoskeleton may play a physiologically important role in posttranscriptional regulation of lymphokine gene expression during early lymphocyte activation.

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Expression of CSF-1 and Its Receptor CSF-1R in Non-Hematopoietic Neoplasms

Kascinski

2002

Ovarian Cancer

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Post-transcriptional regulation of c-fms proto-oncogene expression by dexamethasone and of CSF-1 in human breast carcinomas in vitro

Cited by 19 publications

References 45 publications

Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer

Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer

Interaction of AU-rich sequence binding proteins with actin: Possible involvement of the actin cytoskeleton in lymphokine mRNA turnover

Expression of CSF-1 and Its Receptor CSF-1R in Non-Hematopoietic Neoplasms

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