Post-transcriptional gene silencing: Basic concepts and applications

Ashfaq, Mohd Ashraf; Kumar, Vikash; Pp, Reddy; Kumar, Ch. Ravi; Kumar, Kamal; Rao, Narayana N.; Muddanuru, Tarakeswari; Sujatha, M.

doi:10.1007/s12038-020-00098-3

Cited by 20 publications

(14 citation statements)

References 51 publications

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“…RNAi is a post-transcriptional gene silencing mechanism that is a naturally occurring pathway found in eukaryotic organisms to protect against viruses and/or pathogens producing aberrant RNA molecules (Ashfaq et al, 2020). This process involves the recognition of the aberrant RNA, which is converted into dsRNA (Waterhouse et al, 2001).…”

Section: Rnai (Gene Silencing)mentioning

confidence: 99%

Synthetic biology and opportunities within agricultural crops

Sargent

Conaty

Tissue

et al. 2022

J of Sust Agri & Env

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Conventional breeding techniques have been integral to the development of many agronomically important traits in numerous crops. The adoption of modern biotechnology approaches further advanced and refined trait development and introduction beyond the scope possible through conventional breeding. However, crop yields continue to be challenged by abiotic and biotic factors that require the development of traits that are more genetically complex than can be addressed through conventional breeding or traditional genetic engineering. Therefore, more advanced trait development approaches are required to maintain and improve yields and production efficiency, especially as climate change accelerates the incidence of biotic and abiotic challenges to food and fibre crops. Synthetic biology (SynBio) encompasses approaches that design and construct new biological elements (e.g., enzymes, genetic circuits, cells) or redesign existing biological systems to build new and improved functions. SynBio 'upgrades' the potential of genetic engineering, which involves the transfer of single genes from one organism to another. This technology can enable the introduction of multiple genes in a single transgenic event, either derived from a foreign organism or synthetically generated. It can also enable the assembly of novel genomes from the ground up from a set of standardised genetic parts, which can then be transferred into the target cell or organism. New opportunities to advance breeding applications through exploiting SynBio technology include the introduction of new genes of known function, artificially creating genetic variation, topical applications of small RNAs as pesticides and potentially speeding up the production of new cultivars with elite traits. This review will draw upon case studies to demonstrate the potential application of SynBio to improve crop productivity and resistance to various challenges. Here, we outline specific solutions to challenges including fungal diseases, insect pests, heat and drought stress and nutrient acquisition in a range of important crops using the SynBio toolkit.

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Section: Rnai (Gene Silencing)mentioning

confidence: 99%

Synthetic biology and opportunities within agricultural crops

Sargent

Conaty

Tissue

et al. 2022

J of Sust Agri & Env

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“…Post-transcriptional gene silencing (PTGS) is a mechanism whereby gene silencing is mediated and may be open to epigenetic regulation [7]. PTGS exploits cellular mechanisms using transcripts that have sequence similarity to cellular double-stranded RNA (dsRNA) that undergo degradation in-situ.…”

Section: A) Genomics Provide Clues As To the Existence Of Chalcones In Plantsmentioning

confidence: 99%

The Unique Australian Flora, A Veritable Pandora’s Pharmacopeia of Compounds with Therapeutic Biomedical Potential: Are the Chalcones the Geni in The Box?

Melrose¹

2021

GJSFR

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The aim of this review was to highlight the unique biodiversity of the flowering plants and shrubs of Australia and their component chemicals that evolved during the separation of the Australian continent from Gondwanaland. The chemicals produced by these flowering plants provided protection ensuring the survival of the Australian flora which had to contend with often harsh Australian climatic conditions. The diversity of plant phytochemicals produced by these flowering plants reflects the unique diversity of the Australian Flora and these represent a Pharmacological goldmine. It was beyond the scope of this review to cover the full spectrum of these chemical compounds present in Australian plants instead we focused on the chalcones in this review. This compound has a special status in medicinal chemistry as a base intermediate for the synthesis of a large repertoire of polycyclic compounds that display anti-bacterial, antifungal, anti-viral and anti-tumour properties and these are thus of considerable interest in Biomedicine.

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“…More importantly, they must be still combined with other technologies to perturb the levels of the protein of interest (POI) and assess its biological role throughout the cell cycle. Despite the advancements of genetic tools such as CRISPR/Cas9-based gene editing 32 , gene silencing 33 , or inducible gene expression approaches 34 , none of these systems displays readiness of activity compatible with the kinetics of cell cycle progression.…”

Section: Introductionmentioning

confidence: 99%

A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis

Palmieri

Capece

Tessari

et al. 2022

Preprint

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The OsTIR1/auxin-inducible degron (AID) system allows selective protein degradation upon exposure to the phytohormone auxin. However, this technology does not allow to study the effect of acute protein depletion selectively in one phase of the cell cycle. Here, we report a new AID system to Regulate OsTIR1 Levels based on the Cell Cycle Status (ROLECCS) for phase-specific target proteolysis. Finally, we applied the ROLECCS technology to show that the tumor suppressor TP53 plays a S/G2-specific role in suppression of micronuclei accumulation. This new tool allows the analysis of different protein functions during cell cycle progression with unprecedented temporal resolution.

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Post-transcriptional gene silencing: Basic concepts and applications

Cited by 20 publications

References 51 publications

Synthetic biology and opportunities within agricultural crops

Synthetic biology and opportunities within agricultural crops

The Unique Australian Flora, A Veritable Pandora’s Pharmacopeia of Compounds with Therapeutic Biomedical Potential: Are the Chalcones the Geni in The Box?

A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis

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