Post-transcriptional control of hsp70 mRNA in Trypanosoma brucei

Häusler, Thomas; Clayton, Christine

doi:10.1016/0166-6851(95)02538-3

Cited by 40 publications

(25 citation statements)

References 59 publications

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“…While the potential actin promoter region coincides with an H4K10ac-rich region, the HSP70 promoter does not, suggesting that the HSP70 promoter was misassigned, which agrees with a previous study that failed to confirm promoter activity for the HSP70 promoter (Hausler and Clayton 1996).…”

Section: Histone H4k10ac Is Enriched At Probable Pol II Tssssupporting

confidence: 80%

Nanocrystalline materials

2006

Nano Today

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Section: Histone H4k10ac Is Enriched At Probable Pol II Tssssupporting

confidence: 80%

Nanocrystalline materials

2006

Nano Today

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“…cruzi epimastigotes respond to HSP90 inhibition with a substantial increase of other HSP. All the published work suggests that heat shock gene regulation in protozoa of the order Kinetoplastida does not involve activation of transcription by transcription factors (2,7). We have to presume that members of the Kinetoplastida regulate differential gene expression mostly at a posttranscriptional level (5).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of HSP90 in Trypanosoma cruzi Induces a Stress Response but No Stage Differentiation

Graefe¹,

Wiesgigl

Gaworski³

et al. 2002

Eukaryot Cell

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The 90-kDa heat shock proteins (HSP90) are important in the regulation of numerous intracellular processes in eukaryotic cells. In particular, HSP90 has been shown to be involved in the control of the cellular differentiation of the protozoan parasite Leishmania donovani. We investigated the role of HSP90 in the related parasite Trypanosoma cruzi by inhibiting its function using geldanamycin (GA). GA induced a dose-dependent increase in heat shock protein levels and a dose-dependent arrest of proliferation. Epimastigotes were arrested in G 1 phase of the cell cycle, but no stage differentiation occurred. Blood form trypomastigotes showed conversion towards spheromastigote-like forms when they were cultivated with GA, but differentiation into epimastigotes was permanently blocked. We conclude that, similar to leishmanial HSP90, functional HSP90 is essential for cell division in T. cruzi and serves as a feedback inhibitor in the cellular stress response. In contrast to L. donovani cells, however, T. cruzi cells treated with GA do not begin to differentiate into relevant life cycle stages.

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“…The diminished SOD mRNA level in procyclic trypanosomes may be attributed to a cell density effect. Previously it was shown that mRNA levels are markedly reduced in late-logarithmic-phase procyclic trypanosomes [20].…”

Section: Discussionmentioning

confidence: 99%

Identification of a developmentally regulated iron superoxide dismutase of Trypanosoma brucei

Kabiri

Steverding

2001

Biochemical Journal

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An iron superoxide dismutase (FeSOD) gene of the protozoan parasite Trypanosoma brucei has been cloned and its gene product functionally characterized. The gene encodes a protein of 198 residues which shows 80% identity with FeSODs from other trypanosomatids. Inhibitor studies with purified recombinant FeSOD expressed in Escherichia coli confirmed that the enzyme is an iron-containing SOD. The FeSOD is developmentally regulated in the parasite, expression being lowest in the cell-cycle-arrested, short stumpy bloodstream forms. Differential expression of the FeSOD protein contrasts with only minor quantitative changes in the FeSOD mRNA, indicating post-transcriptional regulation of the enzyme. As the level of FeSOD increases during differentiation of cell-cycle-arrested short stumpy into dividing procyclic forms, it is suggested that the enzyme is only required in proliferating stages of the parasite for the elimination of superoxide radicals which are released during the generation of the iron-tyrosyl free-radical centre in the small subunit of ribonucleotide reductase.

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Post-transcriptional control of hsp70 mRNA in Trypanosoma brucei

Cited by 40 publications

References 59 publications

Nanocrystalline materials

Nanocrystalline materials

Inhibition of HSP90 in Trypanosoma cruzi Induces a Stress Response but No Stage Differentiation

Identification of a developmentally regulated iron superoxide dismutase of Trypanosoma brucei

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