Post-thaw survival and pregnancy rates of biopsied, sexed and vitrified bovine IVF embryos

Ağca, Yüksel; Monson, R.L.; Northey, D.L.; Schaefer, D.M.; Rutledge, J.J.

doi:10.1016/0093-691x(94)00002-c

Cited by 8 publications

(3 citation statements)

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“…However, the importance of an intact zona pellucida as a prerequisite for cryopreserva¬ tion has been questioned (Picard et al, 1988;Garrisi et al, 1992;Schmidt et al, 1992;Loskutoff et al, 1993;Agca et al, 1995;Booth et al, 1996) and development in vitro after vitrification and in-straw direct rehydration has been found to be acceptable (Vajta et al, 1996a, c).…”

Section: Introductionmentioning

confidence: 96%

Survival and development of bovine blastocysts produced in vitro after assisted hatching, vitrification and in-straw direct rehydration

Vajta¹,

Holm²,

Greve³

et al. 1997

Reproduction

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The purpose of this study was to establish an efficient combination of assisted hatching and cryopreservation procedures for producing bovine embryos in vitro. A total of 1312 day 7 blastocysts were subjected randomly to 14 different combinations of three factors: osmotic stress, assisted hatching and vitrification. Re-expansion, initiation and completion of the hatching process, as well as attachment to the culture dish, were analysed by SAS Genmod procedure. Incubation with sucrose was found to decrease survival rates; among the assisted hatching procedures used, zona fenestration resulted in higher survival rates compared with partial zona dissection and controls; and vitrification decreased survival and further development. The combined effect of sucrose incubation and vitrification decreased further development markedly, as did partial zona dissection followed by vitrification. Partial zona dissection performed in medium containing sucrose severely lowered embryo survival. Zona fenestration without sucrose incubation followed by vitrification did not compromise further embryo development: 86%, 84% and 79% of the blastocysts initiated, completed hatching and attached to the bottom, respectively. These data were not different from the controls (80%, 76% and 63%, respectively; P > 0.05). Cell count analysis revealed a decrease in the total number of cells as a result of the assisted hatching and vitrification compared with controls (135 versus 202, respectively; P < 0.0001). Although embryo transfer results (36% pregnancy rate and 30% calving rate) require further improvement, this combination of methods may prove useful in the commercial production of bovine embryos in vitro.

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Section: Introductionmentioning

confidence: 96%

Survival and development of bovine blastocysts produced in vitro after assisted hatching, vitrification and in-straw direct rehydration

Vajta¹,

Holm²,

Greve³

et al. 1997

Reproduction

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show abstract

“…The authors also noted that improved pregnancy results along with more identified genes associated with particular diseases or inherited characteristics could be used for preimplantation genetic diagnostics (Huhtinen et al, 1997). A biopsy technique that utilized a micro-needle to remove a smaller portion of the embryo was described by Agca et al in 1995. Embryos were held in place by suction applied to a glass holding pipette. The pipette was controlled by a joystick-type micromanipulator.…”

Section: Embryo Biopsymentioning

confidence: 99%

“…An aspiration pipette was then inserted through the zona pellucida and 2-3 blastomeres were removed by suction. The cells were left in the media drop and later recovered for genetic testing (Agca et al, 1995).…”

Section: Embryo Biopsymentioning

confidence: 99%

The Effect of Embryo Biopsy and Vitrification on the Development Potential of Equine Embryos

Gearhart¹

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The Effect of Embryo Biopsy and Vitrification on the DevelopmentPotential of Equine EmbryosRichard O. GearhartThis study investigated the development potential of equine embryos in vitro after biopsy and vitrification. Twenty embryos were obtained from Quarter Horse, Thoroughbred, and mix-breed light mares between three and ten years old. The twenty embryos were divided into a biopsy (n=10) and control group (n=10). The biopsy group underwent microaspiration biopsy using a micromanipulator to obtain a small tissue sample from the embryo.Both groups were then vitrified using a commercially available technique originally described by Carnevale (2006) at Colorado State University.All 20 embryos were cultured in DMEM/Hams F-12 medium under oil at 37°C in 5% CO 2 in air (Hinrichs et al., 1990). Embryos were monitored for expansion and hatching. Embryo development was statistically different between the two groups (p<0.05). The biopsy procedure did result in a much lower development potential in the biopsy group as compared to the control group (20% vs. 80%). However, embryos in the biopsy group did show expansion and hatching therefore the combined procedure did not preclude development potential in vitro. Based on these v findings, more research needs to be done to increase the success of the combined procedure and the ultimate viability of the embryos needs to be confirmed with the establishment of successful pregnancies.

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Pregnancies, calves and calf viability after transfer of in vitro produced bovine embryos

et al. 1996

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Post-thaw survival and pregnancy rates of biopsied, sexed and vitrified bovine IVF embryos

Cited by 8 publications

References 0 publications

Survival and development of bovine blastocysts produced in vitro after assisted hatching, vitrification and in-straw direct rehydration

Survival and development of bovine blastocysts produced in vitro after assisted hatching, vitrification and in-straw direct rehydration

The Effect of Embryo Biopsy and Vitrification on the Development Potential of Equine Embryos

Pregnancies, calves and calf viability after transfer of in vitro produced bovine embryos

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