Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

Abbasi, Imtiaz Hussain Raja; Abbasi, Farzana; El‐Hack, Mohamed E. Abd; Swelum, Ayman A.; Yao, Junhu; Cao, Yangchun

doi:10.1186/s13568-018-0566-7

Cited by 15 publications

(8 citation statements)

References 36 publications

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“…Silva et al (2016) reported that dietary CP levels did not affect ruminal pH in finishing beef cattle. Abbasi et al (2018b) noted that different levels of RPMet with low CP could not significantly alter rumen pH before or after feeding. Furthermore, cellulose is the primary building component of plant matter microorganisms recycling them under anaerobic environment and extract energy (Vodovnik and Marinsek 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The dietary RPMet ratio of each ration was estimated based on the included ingredients using the Cornell, Penn-Miner Dairy (CPM, Dairy, and Version 3.0.8.1) Software. The total and individual VFA concentrations of each sample were determined through gas chromatography (Agilent Technology 7820A GC system, Santa Clara, USA) using a 30 m × 0.25 mm × 0.33-m fused silica column (Atech Tech, Co., Ltd. PR, China) followed the procedure of Abbasi et al (2018b). The concentration of gases was analyzed by gas chromatography (model 663-30, Hitachi Corporation, Tokyo, Japan) equipped with a flame ionization detector according to Lambert and Zitomer (1960).…”

Section: Methodsmentioning

confidence: 99%

“…The pH values before or after feeding were measured immediately using pH electrode (Mettler, Toledo Ltd, England, and the UK). The nutrients disappearance rate was calculated according to Abbasi et al (2018b).…”

Section: Methodsmentioning

confidence: 99%

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Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

Abbasi

Liu

et al. 2019

AMB Expr

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The present study was performed to evaluate the effects of different concentration of rumen-protected methionine (RPMet) with a low level of crude protein (CP) using rumen simulation technology on many parameters. The experiment was assigned randomly into four treatments: (1) high protein diet (163.39 g/kg CP) without RPMet (HP); (2) low protein diet (146.33 g/kg CP) without RPMet (LP); (3) low protein diet, supplement with low RPMet (RPMet: 0.11 g/kg) (LPLMet); and (4) low protein diet, supplement with high RPMet (RPMet: 0.81 g/kg) (LPHMet), mixed with 20 g basal diet in each fermenter. Based on National Research Council (NRC) (Nutrient requirements of dairy cattle, National Academies Press, Washington, DC, 2001 ) recommendation for dairy ruminants HP diet was formulated as positive normal control and LP as a negative control. Results demonstrated that CP disappearance was found significantly higher (P < 0.05) in supplement groups compared with HP and found similar (P > 0.05) with LP. However, neutral detergent fiber (NDF) and gross energy (GE) were found a parallel among supplement groups compared to HP and higher than LP. Furthermore, microbial crude protein, total and short chain fatty acids were found similar in LPHMet and HP and found significantly higher than LPLMet and LP. The R. albus population of LPHMet found parallel to HP and pointedly higher than LP in a solid and liquid fraction. Daily production of ammonia nitrogen, total gas, and methane were higher in HP than LP, LPLMet, and LPHMet. Overall, results concluded that values of digestibility, rumen fermentation, microbial crude protein, and R. albus population were similar of LPHMet to that of HP group. However, production of ammonia-N, total gas, and methane volume were significantly higher in the HP group than LPLMet, LPHMet, and LP groups. In conclusion, rumen-protected methionine is a good feed supplement to low dietary protein in the level of 0.81 g/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

Abbasi

Liu

et al. 2019

AMB Expr

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“…Therefore, attempts to protect AA with fat coatings have been considered. However, these approaches have produced inconsistent results 5,16,17 and their efficacy appears limited, mainly due to the poor ruminal protection that is potentially further decreased by mechanical processes such as mastication 18 . Similar approaches relying on pH resistant polymers 19 have also been considered, but appear to suffer from similar limitations 12 .…”

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confidence: 99%

Applying nanotechnology to increase the rumen protection of amino acids in dairy cows

Albuquerque

Casal

Páscoa

et al. 2020

Sci Rep

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the amino acid requirements of high-production dairy cows represent a challenge to ensuring that their diet is supplied with available dietary resources, and thus supplementation with protected amino acids is necessary to increase their post-ruminal supply. Lysine is often the most limiting amino acid in cornbased diets. the present study proposes the use of lipid nanoparticles as novel rumen-bypass systems and assesses their capability to carry lysine. Solid lipid nanoparticles, nanostructured lipid carriers and multiple lipid nanoparticles were considered and their resistance in a rumen inoculum collected from fistulated cows was assessed. All nanoparticles presented diameters between 200-500 nm and surface charges lower than −30 mV. Lysine encapsulation was achieved in all nanoparticles, and its efficiency ranged from 40 to 90%. Solid lipid nanoparticles composed of arachidic or stearic acids and Tween 60 resisted ruminal digestion for up to 24 h. The nanoparticles were also proven to protect their lysine content from the ruminal microbiota. Based on our findings, the proposed nanoparticles represent promising candidates for rumen-bypass approaches and should be studied further to help improve the current technologies and overcome their limitations.

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“…Враховуючи все вищезгадане використання захищеного метіоніну, в якості харчової добавки до раціону тварин, може забезпечити дефіцит амінокислот у організмі (Abbasi et al, 2018). Метіонін представляє собою сірковмісну амінокислоту, яка бере участь у синтезі фосфоліпідів, поліамінів, вітаміну А, карнітину.…”

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Dietary Impacts on Early Rumen Microbiota Development in Calves

Шкромада

Улько

Udovenko

2020

SCIVP

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The rumen is an important digestive organ in which fermentation processes take place. Improving the rumen development of growing cattle is one of the major tasks. The need for ruminants in amino acids is as great as in mono-gastric animals. The rumen microorganisms cannot synthesize microbial protein in sufficient quantity to cover the need of developmental growth. The experiments were conducted on calves from four to five months of age at the «Za myr» farm in Sumy district, Sumy region. The calves were divided into three experimental groups of 10 animals each. The first experimental group was fed powder not protected methionine at a dose of 10 grams per animal, the second group was fed granulated methionine at a dose of 10 grams per animal for a month. The results suggest that bacterial count increased by 84,3%; Paramecium caudatum by 29,4 %; Entodiniomorpha by 26,6 % in the first experimental group to the 30th day. In the second group the indicators also changed after a month of research. The bacterial count was 93,2 % higher; Paramecium caudatum by 42,5 %; Entodiniomorpha by 25,9 % compared to control. According to the results of the experiment, we can assumed that powder methionine as well as granular had a positive effect on the qualitative and quantitative composition of the rumen microflora. In the first experimental group content of total nitrogen was higher by 12,2 % and protein content by 19,6 % compared to controls. In the second experimental group, where calves were fed a granulated methionine, content of total nitrogen was higher by 15,6 % and protein content by 22,4 % compared to controls. The residual nitrogen concentration significantly decreased by 32,2 % in the first group of calves and by 29,9 % in the second. It was experimentally proved that the enriched of granulated and non-granulated methionine food additives helped to increase the number of microorganisms in the rumen and increase the concentration of nitrogen in the rumen.

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Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

Cited by 15 publications

References 36 publications

Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

Applying nanotechnology to increase the rumen protection of amino acids in dairy cows

Dietary Impacts on Early Rumen Microbiota Development in Calves

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