Post-prandial lipemia and glycemia in dogs fed with industrialized pet food

Silva, N. L. T.; Bonatto, Natália Camila Minucci; Oliveira, Paula Lima de; Vieira, Guilherme Coutinho; Floriano, Beatriz Perez; Barros, Luiz Daniel de; Bosculo, Maria Rachel Melo; Almeida, Breno Fernando Martins de

doi:10.1007/s00580-018-2824-0

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…Hemolysis and lipemia are also common confounders for blood-based tests. Study subjects were enrolled without any restrictions related to the timing of the dog’s last feeding, and lipemia scores (which serve as a proxy for the timing of the most recent meal) [ 103 ] had no significant association with test performance. Likewise, hemolysis scores had no significant association with test performance.…”

Section: Discussionmentioning

confidence: 99%

Clinical validation of a next-generation sequencing-based multi-cancer early detection “liquid biopsy” blood test in over 1,000 dogs using an independent testing set: The CANcer Detection in Dogs (CANDiD) study

Flory

Kruglyak²,

Tynan³

et al. 2022

PLoS ONE

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Cancer is the leading cause of death in dogs, yet there are no established screening paradigms for early detection. Liquid biopsy methods that interrogate cancer-derived genomic alterations in cell-free DNA in blood are being adopted for multi-cancer early detection in human medicine and are now available for veterinary use. The CANcer Detection in Dogs (CANDiD) study is an international, multi-center clinical study designed to validate the performance of a novel multi-cancer early detection “liquid biopsy” test developed for noninvasive detection and characterization of cancer in dogs using next-generation sequencing (NGS) of blood-derived DNA; study results are reported here. In total, 1,358 cancer-diagnosed and presumably cancer-free dogs were enrolled in the study, representing the range of breeds, weights, ages, and cancer types seen in routine clinical practice; 1,100 subjects met inclusion criteria for analysis and were used in the validation of the test. Overall, the liquid biopsy test demonstrated a 54.7% (95% CI: 49.3–60.0%) sensitivity and a 98.5% (95% CI: 97.0–99.3%) specificity. For three of the most aggressive canine cancers (lymphoma, hemangiosarcoma, osteosarcoma), the detection rate was 85.4% (95% CI: 78.4–90.9%); and for eight of the most common canine cancers (lymphoma, hemangiosarcoma, osteosarcoma, soft tissue sarcoma, mast cell tumor, mammary gland carcinoma, anal sac adenocarcinoma, malignant melanoma), the detection rate was 61.9% (95% CI: 55.3–68.1%). The test detected cancer signal in patients representing 30 distinct cancer types and provided a Cancer Signal Origin prediction for a subset of patients with hematological malignancies. Furthermore, the test accurately detected cancer signal in four presumably cancer-free subjects before the onset of clinical signs, further supporting the utility of liquid biopsy as an early detection test. Taken together, these findings demonstrate that NGS-based liquid biopsy can offer a novel option for noninvasive multi-cancer detection in dogs.

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Section: Discussionmentioning

confidence: 99%

Clinical validation of a next-generation sequencing-based multi-cancer early detection “liquid biopsy” blood test in over 1,000 dogs using an independent testing set: The CANcer Detection in Dogs (CANDiD) study

Flory

Kruglyak²,

Tynan³

et al. 2022

PLoS ONE

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“…Biochemical analysis was performed as part of a health screening for inclusion in this study, according to previously published methodology (COSTA et al, 2020, p. 2222, SILVA, et al, 2019).…”

Section: Laboratory Analysismentioning

confidence: 99%

“…Insufficient fasting (less than 8 hours) induces blood sample lipemia due to accumulation of postprandial chylomicrons, producing turbidity in the serum or plasma, mainly when triglyceride levels exceed 300 mg/dL (BAUER, 2004, 672, THRALL et al, 2012. In healthy dogs fed with commercial feed, postprandial lipemia has been shown to occur from 2h to 5h, with maximum mean value at 3h (SILVA et al, 2019). However, little is known regarding the isolated effects of feeding on hematological parameters of dogs.…”

Section: Introductionmentioning

confidence: 99%

Effect of food intake on complete blood count of healthy dogs

Oliveira¹,

Souza²,

Bonatto³

et al. 2020

Rev Agr Acad

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The present determined the hematological alterations of healthy dogs at the peak of postprandial hypertriglyceridemia. Twenty-four clinically healthy dogs had blood samples collected to perform complete blood count on three consecutive days at the same time every day: first day after a 12-hour fast; second day three hours after feeding with commercial feed, during the peak of postprandial lipemia; and third day after a 12-hour fast. Feeding led to an increase in MCHC, hemoglobin and white blood cell count due to the increase in segmented neutrophil, monocyte and eosinophil concentrations. A significant increase in total plasma protein content was also observed. Postprandial condition at the peak of hypertriglyceridemia influences hematological parameters of healthy dogs, which is an important finding, however transient, when interpreting laboratory blood tests.

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