Post-natal changes in the biological characteristics of Semitendinosus muscle in male Limousin cattle

Jurie, Catherine; Robelin, J.; Picard, Brigitte; Geay, Y.

doi:10.1016/0309-1740(94)00074-h

Cited by 52 publications

(40 citation statements)

References 38 publications

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“…So according to the fact that the ST muscle of cloned heifers is slower and more oxidative than that of control heifers at 8 months of age, a delayed maturation was supposed. The results from this study, namely the LDH/ICDH ratio, observed in clones at 8 months of age were similar to that described in control animals at 1 month of age in a previous study (Jurie et al, 1995), and the observed values for COX and HAD activities, another enzyme reflecting the oxidative pathway, were greater for cloned than for control heifers at 8 months of age, which was in agreement with this hypothesis. In addition, this delay probably originates from foetal myogenesis, since early in foetal life, a higher proportion of primary myotubes, and just before birth at 260-day post-conception the presence of foetal MyHC isoform, a lack of adult MyHC isoforms and a higher oxidative metabolism were observed in the ST muscle from cloned Holstein foetuses compared with control Charolais foetuses (Picard et al, 2006b).…”

Section: Discussionsupporting

confidence: 92%

“…This indicates that fibres classified IIB by conventional histochemical (Picard et al, 1999). During early postnatal growth (up to about 6 months of age), there is a conversion in fibre types, namely from fast oxido-glycolytic fibres (type FOG or IIA) into fast glycolytic fibres (type FG or IIX), which indicates a modification of muscle metabolism (Jurie et al, 1995;Wegner et al, 2000). From 6 to 12 months of age, there is a slowing down of this conversion, the rate of which is dependent on the breed (Wegner et al, 2000).…”

Section: Discussionmentioning

confidence: 95%

“…A conversion from fast to slow muscle fibre types was described from 19 months of age onwards in young bulls (Maltin et al, 1998;Jurie et al, 2005), indicating a modification of contractile type. These changes in the frequencies of different fibre types with age are accompanied by changes in area and percentage of total muscle area of each fibre type, and in muscular metabolism as indicated by metabolic enzyme activities (Jurie et al, 1995 and. Thus, in the muscles of young bulls, LDH activity (glycolytic metabolism) increases and ICDH activity (oxidative metabolism) decreases after birth.…”

Section: Discussionmentioning

confidence: 99%

“…Picard et al (2006a) have reported that muscular metabolism is the most oxidative around birth than later; maximal values were observed at 260-day post-conception and 3-week post-natal. Then during the first months of life, it becomes more glycolytic (Jurie et al, 1995). So according to the fact that the ST muscle of cloned heifers is slower and more oxidative than that of control heifers at 8 months of age, a delayed maturation was supposed.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Jurie

Picard

Heyman

et al. 2009

Animal

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Muscle contractile and metabolic characteristics were studied on nine cloned and eight non-cloned (control) heifers. The animals were submitted to repeated biopsies of the semitendinosus (ST) muscle at the ages of 8, 12, 18 and 24 months. The contractile type was determined from the proportion of the different myosin heavy chain (MyHC) isoforms separated by electrophoresis. Glycolytic metabolism was assessed by lactate dehydrogenase (LDH) activity, and oxidative metabolism was assessed by isocitrate dehydrogenase (ICDH), cytochrome-c oxidase (COX) and b-hydroxyacyl-CoA dehydrogenase (HAD) activities. In cloned heifers at 8 months of age, there was a greater proportion of MyHC I (slow oxidative isoform) and MyHC IIa (fast oxido-glycolytic isoform), a lower proportion of MyHC IIx (fast glycolytic isoform), greater COX and HAD activity and a lower LDH/ICDH ratio compared with control heifers. Thus, young cloned heifers had slower muscle types associated with a more oxidative muscular metabolism than control heifers. From 12 months of age onwards, no significant differences were observed between cloned and control heifers. A delay in muscle differentiation and maturation in cloned heifers is hypothesised and discussed.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Jurie

Picard

Heyman

et al. 2009

Animal

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“…As metabolic muscle parameters are important for meat quality, lactate dehydrogenase (LDH) and isocitrate dehydrogenase (ICDH) activities were measured as described by Jurie et al (1995) to assess glycolytic and oxidative metabolism, respectively. Values were compared between clones and controls at different ages using the t-test.…”

Section: Muscle Analysismentioning

confidence: 99%

Quality and safety of bovine clones and their products

Heyman

Chavatte‐Palmer

Fromentin³

et al. 2007

Animal

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A multidisciplinary research programme was developed to get a scientific expertise for the quality assessment of products obtained from cloned livestock. Thirty-seven bovine Holstein female clones of five different genotypes and their products were analysed in comparison with 38 control animals obtained by conventional artificial insemination and raised under the same conditions at the same experimental farm. Animal evaluation included over 150 criteria and more than 10 000 measurements to check the physiological status and health over a 3-year period. All the parameters studied were in the normal range for age and breed, but some significant differences were detected between clone and control groups in terms of delayed onset of puberty in clones, higher neutrophil counts in haematology or lower biochemical plasma concentrations of gamma glutamyl transferase. Milk and meat analyses were conformable to expected values. We, however, found some differences in fatty acid (FA) composition of milk and muscle suggesting a possible deviation in lipid metabolism as assessed by higher delta-9 desaturase activity indexes in both milk and muscles from clones compared with controls. Repeated muscle biopsies in the semitendinosus muscle of the same animals demonstrated a higher oxidative activity in muscle of young clones (8 months of age) compared with controls, suggesting a delayed muscle maturation in clones. Nutritional evaluation of milk and meat using the rat feeding trials did not show any difference between clone and control products for food intake, growth rate, body composition of the rats, nor for possible allergenicity. Possible reactivation of bovine endogenous retroviruses (BERVs) was analysed and compared between normal and cloned cattle. As expected, these BERV sequences are not transcribed and no RNA was detected in the blood of clones, donor animals or controls; therefore, it may be assumed that the sanitary risk associated with BERV sequences is not higher in cattle derived from somatic nuclear transfer than in cattle born from conventional reproduction. Our results confirm that the quality and safety of products (milk and meat) from adult and clinically healthy cloned cattle is globally similar to normal animals. However, from a strictly biological point of view, the slightly delayed maturation we observed in the muscle of clones together with some marginal differences identified in FA composition of both muscle and milk, point to the need for more refined analysis to totally exclude any risks from the consumption of those products.

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Consequences of selection for higher growth rate on muscle fibre development in cattle

et al. 2006

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Post-natal changes in the biological characteristics of Semitendinosus muscle in male Limousin cattle

Cited by 52 publications

References 38 publications

Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Quality and safety of bovine clones and their products

Consequences of selection for higher growth rate on muscle fibre development in cattle

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