Post-Ischemic 31P Nmr Determination of Myocardial Intracellular Ph in vivo Using Atp Peak

Houston, R.; Heerschap, Arend; Skotnicki, S.H.; Verheugt, Freek W. A.; Oeseburg, B.

doi:10.1007/978-1-4615-5399-1_35

Cited by 2 publications

(1 citation statement)

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“…The metabolic ratios of -, -, -ATP, PCr, Pi, PDE To calculate pH from the -ATP peak, the following formula, introduced by Houston et al (20), was used:…”

Section: Methodsmentioning

confidence: 99%

(31)P MR Spectroscopic Measurement of Intracellular pH in Normal Human Hearts

Kwon

Lee

Jang

et al. 2002

J Korean Radiol Soc

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Purpose:To assess the usefulness of intracellular pH (pHi), calculated by determining the shift of a high-energy metabolite such as inorganic phosphate (Pi) or -ATP after performing MRS with ECG-gated two-dimensional 31 P CSI (chemical shift imaging), as a parameter for the overall state of the intracellular milieu. Materials and Methods: Proton decoupled 31 P CSI was performed on a 1.5-T scanner using a 1 H-31 P dual-tuned surface coil. Cardiac MRS data were obtained from eight normal volunteers aged 24 32 years with no history of heart disease. From the spectra obtained from several regions of the heart, peak position and peak area were estimated. The metabolic ratios of -, -, -ATP, PCr, Pi, phosphodiester and diphosphoglycerate were calculated, and pHi was estimated from the chemical shift of Pi and -ATP resonance. We then compared the data for the anterior myocardium with those previously published. Results: The major phosphorous metabolites identified in these human hearts were as follows: PCr, at 0.1 to +0.1 ppm; three phosphate peaks from ATP, with a chemical shift centered at about 2.7 ppm ( -ATP), 7.8 ppm ( -ATP), and 16.3 ppm ( -ATP); and phosphodiester (PDE) at 2 3 ppm, inorganic phosphate (Pi) at 4.5 5.4 ppm, and diphosphoglycerate (DPG) at 5.4 6.3 ppm. The PCr/ -ATP ratio was 2.20 0.17 and the PDE/ -ATP ratio, 1.04 0.09. pHi readings were 7.31 0.23 (calculated by the shift of Pi) and 6.81 0.20 (calculated by the shift of -ATP). Pi/PCR was 0.539, a ratio higher than that mentioned in previously published reports. Conclusion: The measurement of intracelluar metabolism was affected by various kinds of factors. We believe, however, that pHi readings indicate the overall state of the cardiac intracelluar milieu. An unexpected pHi readings, seen at MRS, may reflect errors in the MR procedure itself and, or in the alanytical method.

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“…The metabolic ratios of -, -, -ATP, PCr, Pi, PDE To calculate pH from the -ATP peak, the following formula, introduced by Houston et al (20), was used:…”

Section: Methodsmentioning

confidence: 99%