Post-injury regeneration in rat sciatic nerve facilitated by neurotrophic factors secreted by amniotic fluid mesenchymal stem cells

Pan, Hung-Chuan; Cheng, Fu‐Chou; Chen, Chun‐Jung; Lai, Shu-Zhen; Lee, Chi-Wen; Yang, Dar-Yu; Chang, Ming-Hong; Ho, Shu-Peng

doi:10.1016/j.jocn.2006.08.008

Cited by 138 publications

(174 citation statements)

References 43 publications

(55 reference statements)

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“…To our knowledge, there are no reports in the literature of such an in vivo ionic flux regulation by MSC in the GIT. Some in vivo electrophysiological experiments have revealed the capacity of MSC to increase conductance velocity (reduction of conductance latency and enhancement of action potential number and amplitude) of motor nerves 14 and somatosensory nerves 15 for functional recovery of injured peripheral nervous system. Moreover, an in vitro study has shown that MSC are able to repair cross-channel electrical conduction blocking in cardiomyocytes.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells improve small intestinal integrity through regulation of endogenous epithelial cell homeostasis

et al. 2009

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Patients who undergo pelvic or abdominal radiotherapy may develop acute and/or chronic side effects resulting from gastrointestinal tract (GIT) alterations. In this study, we address the question of the regenerative capability of mesenchymal stem cells (MSC) after radiation-induced GIT injury. We also propose cellular targets of MSC therapy. We report that the infusion of human bone marrow-derived MSC (hMSC) provides a therapeutic benefit to NOD/SCID mice undergoing radiation-induced GIT failure. We observed that hMSC treatment brings about fast recovery of the small intestine (structure and function) in mice with reversible alterations and extends the life of mice with irreversible GIT disorders. The effects of hMSC are a consequence of their ability to improve the renewal capability of small intestinal epithelium. hMSC treatment favors the re-establishment of cellular homeostasis by both increasing endogenous proliferation processes (Ki67 immunostaining) and inhibiting apoptosis (TUNEL staining) of radiation-induced small intestinal epithelial cells. Our results suggest that MSC infusion may be used as a therapeutic treatment to limit radiation-induced GIT damage.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells improve small intestinal integrity through regulation of endogenous epithelial cell homeostasis

et al. 2009

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“…No difference in functional, morphometric or immunohistochemistry between ADSCs and BMSCs Erba et al [45] Rat sciatic transection (10 mm gap) U Fibrin PHB conduit Lack of sufficient quantities of viable cells 14-d after transplantation; conclusion that regenerative effect due to initial growth factor boost or paracrine effect on resident cells Sun et al [51] Rat facial transection (8 mm gap) D Matrigel Decellularized allogeneic artery dADSCs persisted at repair site and integrated with regenerated tissue; conduits containing dADSCs achieved results comparable to those of SC-containing conduits and superior to matrigel-containing conduits alone; results inferior to autograft Fetal Pan et al [106] Rat sciatic crush U Fibrin glue…”

Section: Vein Graftmentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

124

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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“…6 Nevertheless, incidence of differentiation from naive precursor cells within the peripheral nerve is rather low in many cases. 10,11,18,46 Choosing to predifferentiate stem cells toward a desired phenotype prior to delivery into the repair site may be an effective strategy to ensure a more precise and complete therapeutic effect. It may be that cells at later developmental stages (vs embryonic stem cells, for example) possess more mature intrinsic molecular programs to direct them to their target destination.…”

Section: Differentiation State Of Delivered Stem Cellsmentioning

confidence: 99%

“…The question of survival is mechanistically interesting, as improvement in regeneration outcomes has been also been observed in the absence of detection of transplanted cells. 46 If a minimum survival time of stem cells is indeed required to observe a therapeutic effect, strategies should be devised to increase the amount of time cells remain in grafted regions. Survival and effectiveness of transplanted cells can be improved by ex vivo genetic manipulation or concomitant delivery of protective agents or trophic factors.…”

Section: Improving Survival Of Transplanted Stem Cellsmentioning

confidence: 99%

Practical considerations concerning the use of stem cells for peripheral nerve repair

Walsh

Midha

2009

FOC

111

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In this review the authors intend to demonstrate the need for supplementing conventional repair of the injured nerve with alternative therapies, namely transplantation of stem or progenitor cells. Although peripheral nerves do exhibit the potential to regenerate axons and reinnervate the end organ, outcome following severe nerve injury, even after repair, remains relatively poor. This is likely because of the extensive injury zone that prevents axon outgrowth. Even if outgrowth does occur, a relatively slow growth rate of regeneration results in prolonged denervation of the distal nerve. Whereas denervated Schwann cells (SCs) are key players in the early regenerative success of peripheral nerves, protracted loss of axonal contact renders Schwann cells unreceptive for axonal regeneration. Given that denervated Schwann cells appear to become effete, one logical approach is to support the distal denervated nerve environment by replacing host cells with those derived exogenously. A number of different sources of stem/precursor cells are being explored for their potential application in the scenario of peripheral nerve injury. The most promising candidate, transplant cells are derived from easily accessible sources such as the skin, bone marrow, or adipose tissue, all of which have demonstrated the capacity to differentiate into Schwann cell–like cells. Although recent studies have shown that stem cells can act as promising and beneficial adjuncts to nerve repair, considerable optimization of these therapies will be required for their potential to be realized in a clinical setting. The authors investigate the relevance of the delivery method (both the number and differentiation state of cells) on experimental outcomes, and seek to clarify whether stem cells must survive and differentiate in the injured nerve to convey a therapeutic effect. As our laboratory uses skin-derived precursor cells (SKPCs) in various nerve injury paradigms, we relate our findings on cell fate to other published studies to demonstrate the need to quantify stem cell survival and differentiation for future studies.

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Post-injury regeneration in rat sciatic nerve facilitated by neurotrophic factors secreted by amniotic fluid mesenchymal stem cells

Cited by 138 publications

References 43 publications

Mesenchymal stem cells improve small intestinal integrity through regulation of endogenous epithelial cell homeostasis

Mesenchymal stem cells improve small intestinal integrity through regulation of endogenous epithelial cell homeostasis

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Practical considerations concerning the use of stem cells for peripheral nerve repair

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