Post‐hatching developmental changes in the adrenal gland of the Japanese quail (Coturnix coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Abdel-Maksoud, Fatma M.; Abou-Elmagd, A.; Saleh, Abdelmohaimen M.

doi:10.1002/jemt.24348

Cited by 3 publications

(2 citation statements)

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“…It is found in three cell compartments: the plasma membrane, the cytoplasm, and the nucleus (Orsulic et al, 1999). Its expression in the cortical and medullary cells in the current study could be attributed to the loss of demarcation between the cortex and medulla in adrenal of the birds (Abdel‐Maksoud et al, 2023). β‐catenin accumulation in the outer cortex could be involved in establishment of adrenal cortex zonation (Drelon et al, 2015).…”

Section: Discussionmentioning

confidence: 56%

“…In addition, the cells within the capsular zone showed a stronger positive reaction. The strong positive reaction of these cells within the capsular zone may be an indication that there was a high proportion of chromaffin stem cells in that region (Abdel‐Maksoud et al, 2023). Sox10, a member of SOX family of transcription factors (Schepers et al, 2002) serves as a nucleocytoplasmic shuttle protein and is essential for the development of the neural crest and the peripheral nervous system (Cheung et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Prehatching development of the adrenal gland in Japanese quail (Coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Fadl,

Saleh,

Abou‐Elmagd

et al. 2023

Microscopy Res & Technique

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The adrenal glands play a key role in maintaining the physiological balance of birds and helping them to survive environmental changes. The objective of the present work was to give a detailed investigation of the histological, ultrastructural, and immunohistochemical findings of the adrenal gland in Japanese quail during the prehatching phase. The current study was performed on 45 healthy Japanese quail embryos at different prehatching periods. Our results showed the primordium of the quail's adrenocortical tissue appeared at 3 days of incubation as a thickening of the splanchnic mesoderm. The prospective chromaffin cells appeared at 5 days as clusters of cells migrated from the neural crest cells along the dorsal aorta toward the interrenal tissue. TH immunoreactivity was observed in the neural crest cells during their migration toward the adrenal primordium. Furthermore, these TH immunopositive cells were intermingled with the developing interrenal cell cords that developed from the coelomic epithelium. NSE immunostaining was detected within the cytoplasm of interrenal cells, chromaffin cells, and ganglion cells. Sox10 is expressed in chromaffin and ganglion cells with different staining intensities. On the 13th day of prehatching, both interrenal and chromaffin cells were β‐catenin immunonegative, but on the 17th day, both cells were immunopositively. Our findings show that during prenatal life, the adrenal gland undergoes significant morphological changes. Together, the present data suggest that studying the prenatal development of the adrenal gland in birds is important for advancing our understanding of this critical organ and its functions.Research Highlights The present study aimed to give a detailed study of the histological, ultrastructural, and immunohistochemical investigations of the adrenal gland in Japanese quail during the prehatching period. The interrenal primordium was observed on the third embryonic day, on the fifth ED the primordium of the chromaffin tissue appeared as row of migrating neural crest cell. At the ultrastructural level, the interrenal cells take steroid‐secreting cells characters, they have varying amounts of lipid droplets and abundant mitochondria at 15th ED contained moderate number of lysosomes and mitochondria.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Prehatching development of the adrenal gland in Japanese quail (Coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Fadl,

Saleh,

Abou‐Elmagd

et al. 2023

Microscopy Res & Technique

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Meckel’s Diverticulum in Adult Geese (Alopochen egyptiacus): A Comprehensive Study of Structure Using Histological, Electron Microscopy, and Immunohistochemical Methods

Abdel-maksoud,

Ali,

Abd-Elhafeez

et al. 2024

Cells Tissues Organs

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The intestine plays an important role in mediating between the bird and its nutritional environment. The yolk stalk, also known as Meckel's diverticulum, is a landmark between the jejunum and ileum. This work aimed to investigate the anatomical, histological, and electron microscopical features of cellular components of the Meckel’s diverticulum (MD) in adult geese. The intestine was dissected from the bird’s body cavity, and Meckel’s diverticulum was exposed and prepared for light and electron microscopical examinations. Our results revealed that the MD mucosa is thrown up into villi and crypts, and the mucosal epithelium is a columnar epithelium with goblet cells as well as intraepithelial lymphocytes. Lymphoid follicles and numerous immune cells were demonstrated within the lamina propria. The mucous glands were also observed within the lamina propria and amongst the lymphoid follicles. The lining epithelium of MD appeared with different staining affinities: dark cells (electron-dense) and light cells (electron-lucent) contained few mitochondria and more secretory vesicles, while dark cells contained more mitochondria and fewer secretory vesicles. Immunohistochemical analysis of MD revealed positive immunoreactivity for several markers, such as CD 117, chromogranin, PLCβ, cytokeratin, MHC II, and S 100. Taken together, our findings suggest that MD is considered an immune organ in adult geese.

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Morphological and Immunohistochemical Differentiation of Neuronal and Glial Cells of the Vascular and Avascular Regions of the Donkey’s Paurangiotic Retina

Gaber,

Hussein,

Aly

et al. 2024

Cells Tissues Organs

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Introduction: Ocular diseases pose a significant health concern for donkeys. However, studies examining the microanatomy and cell populations of the donkey retina are scarce. The current study aimed to describe the vascular pattern of the donkey retina and document its cellular components. Methods: The donkey retina specimens were obtained from different retinal regions and prepared for semithin sectioning and immunohistochemistry. Results: The donkey has a paurangiotic retina in which retinal vessels are confined to a narrow area around the optic disc. Glial cells coexist with the blood vessels being very numerous in the vascular region and become scanty in the avascular ones. S-100-positive astrocytes could be observed in these avascular areas. Ganglion cells are organized in a single layer with the least population existing in the peripheral retina. Acidic fibroblast growth factor (AFGF) is immunoreactive in amacrine and ganglion cells. A subpopulation of amacrine cells reacted strongly to tyrosine hydroxylase (TH), and others reacted positively to S-100 protein. Ganglion cell nuclei exhibited a strong immunoreactivity to S-100 protein as well. Furthermore, glial fibrillary acidic protein (GFAP) is used to identify Müller cells that extend their processes across the retina from the inner to the outer limiting membrane. Conclusions: In conclusion, our findings provide novel insights into the normal retinal organization. The donkey retina shows the characteristic expression of immunohistochemical markers for the major cell types. In addition, the distribution of glial cells is comparable between the vascular and avascular regions.

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Post‐hatching developmental changes in the adrenal gland of the Japanese quail (Coturnix coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Cited by 3 publications

References 48 publications

Prehatching development of the adrenal gland in Japanese quail (Coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Prehatching development of the adrenal gland in Japanese quail (Coturnix japonica): Histological, immunohistochemical, and electron microscopic studies

Meckel’s Diverticulum in Adult Geese (<i>Alopochen egyptiacus</i>): A Comprehensive Study of Structure Using Histological, Electron Microscopy, and Immunohistochemical Methods

Morphological and Immunohistochemical Differentiation of Neuronal and Glial Cells of the Vascular and Avascular Regions of the Donkey’s Paurangiotic Retina

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