Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection

Skjøt-Rasmussen, Line; Sandvang, Dorthe; Blanch, Alfred; Nielsen, Jette Mundus; Styrishave, Tina; Schnabl, Jannie; Brockmann, Elke; Beck, Chrysta N; Kiess, Aaron S.

doi:10.1093/femsle/fnz078s

Cited by 2 publications

(5 citation statements)

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“…The improvement in FCR and the trend in BW gain, especially by the highest GH concentration (10 7 cfu GH/50 μL), were carried through day 21 of the grow-out period. This could mean that this serotype of E. faecium at a higher concentration can colonize and multiply in the chicken's gastrointestinal tract, thus exerting its beneficial effects for a longer period ( Skjøt-Rasmussen et al., 2019 ). The delivery of the probiotic into the amnion, as compared to the air cell ( Coskun et al., 2015 ), possibly made the probiotic available earlier for the embryo to absorb as suggested by Castañeda et al.…”

Section: Discussionmentioning

confidence: 99%

In ovo inoculation of an Enterococcus faecium–based product to enhance broiler hatchability, live performance, and intestinal morphology

et al. 2020

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Previous studies have suggested the use of probiotics, as alternative to antibiotics, to enhance broiler performance. The administration of probiotics in feed has been widely explored; however, few studies have evaluated the in ovo inoculation of probiotics. Therefore, the objective was to evaluate the impact of in ovo inoculation of different concentrations of GalliPro Hatch ( GH ), an Enterococcus faecium –based probiotic, on hatchability, live performance, and gastrointestinal parameters. Ross x Ross 708 fertile eggs were incubated, and on day 18, injected with the following treatments: 1) 50 μL of Marek's vaccine ( MV ), 2) MV and 1.4 × 10 5 cfu GH/50 μL, 3) MV and 1.4 × 10 6 cfu GH/50 μL, 4) MV and 1.4 × 10 7 cfu GH/50 μL. On the day of hatch, chicks were weighed, feather sexed, and hatch residue was analyzed. Male birds (640) were randomly assigned to 40 floor pens. On day 0, 7, 14, and 21 of the grow-out phase, performance data were collected. One bird from each pen was used to obtain yolk weight and intestinal segment weight and length. Hatchability was not impacted by any GH treatment ( P = 0.58). On day 0, yolk weight was lower for all treatments than for MV alone. On day 0 to 7, feed intake was lower for 10 5 and 10 7 GH; the feed conversion ratio ( FCR ) was lower for all treatments than for MV alone ( P = 0.05; P = 0.01, respectively). From day 14 to 21, the 10 7 GH treatment had higher BW gain ( P = 0.05). For day 0 to 21, 10 7 GH had a lower FCR than MV alone ( P = 0.03). On day 0, all GH treatments resulted in heavier tissues and longer jejunum, ileum, and ceca lengths than MV alone ( P < 0.05). Spleen weight was higher for 10 5 and 10 7 GH than for MV alone. In conclusion, GH does not impact hatchability, and some concentrations improved live performance through the first 21 d of the grow-out phase. These improvements could result from the increased yolk absorption and improved intestinal and spleen morphology seen in this study.

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Section: Discussionmentioning

confidence: 99%

In ovo inoculation of an Enterococcus faecium–based product to enhance broiler hatchability, live performance, and intestinal morphology

et al. 2020

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“…However, in the in ovo injection trial, the control group was injected with Marek's vaccine alone, and hatchability where compared with that of the group injected with Marek's vaccine and the E. faecium probiotic. Based on the results, no significant difference in hatchability was found between these groups (Skjøt-Rasmussen et al, 2019). Only in the experiment in which eggs were dipped in the probiotic solution did the hatchability differ significantly between the control and treated groups (Supplementary Table S1 -Experiments 3 and 4).…”

Section: Hatchabilitymentioning

confidence: 92%

“…Enumeration was used for this comparison of the E. faecium in caeca of 10 chickens (Ross 708), which received the E. faecium probiotic via in ovo injection. These chickens received a concentration of 1.4×10 7 cfu E. faecium/egg into the amniotic fluid as previously described by Skjøt-Rasmussen et al (2019). Their caeca were stored separately in cryotubes at -80 °C until analysis was conducted, together with the caecal samples from the spray experiment described above.…”

Section: Re-isolation Of the Probiotic Enterococcus Faecium From Caeca Of The Chickens' Day 4 And 7 Post-hatch After Application By Spraymentioning

confidence: 99%

“…Only fully hatched chickens were included.Beneficial MicrobesPlease cite this article as 'in press'https://www.wageningenacademic.com/doi/pdf/10.3920/BM2020.0227 -Sunday, August 22, 2021 9:23:45 PM -IP Address:44.224.250.200 received the probiotic on day 18 in the incubation period. The in ovo experiment was conducted at the Mississippi State University, where E. faecium was administered in ovo with Marek's vaccine (Zoetis, Parsippany, NJ, USA) in the amnion cavity(Skjøt-Rasmussen et al, 2019). A selection of these samples was analysed in this study and compared with samples from the spray application (Table5, Supplementary TableS1-Experiment 18).…”

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“…Application of Enterococcus faecium as in ovo injection (Objective 5).a Ten samples were analysed in this study, and the remaining samples were analysed bySkjøt-Rasmussen et al (2019).Please cite this article as 'in press'Beneficial Microbes https…”

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Recovery of probiotic bacteria from the intestinal tract of broilers after noninvasive pre-hatch application

Thøfner

Sandvang

Aagaard

et al. 2021

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This paper reports the success of intestinal colonisation of chickens and foetuses by probiotics after different methods of pre-hatch application. Hatcheries not using in ovo injection of probiotics or wish to avoid the reduced hatchability associated with in ovo injections prefer using alternatives to in ovo technologies. Therefore, we used noninvasive pre-hatch application methods. This included the vertical transmission of probiotics from the mother hen to offspring, application of probiotic late in incubation and transmission of probiotics during hatch. Enterococcus faecium (NCIMB11181) and Lactobacillus animalis (DSM33570) were used as probiotics. Probiotics were applied either through drinking water for the mother hens, by dipping the eggs in a probiotic solution on days 16-18 of incubation or through drops/spray on the eggshell of the fertilised eggs. Similarly, intestinal colonisation of the probiotic in chickens was investigated either before hatch (pre-hatch) or immediately after hatch (post-hatch). Based on the performed experiments, it is concluded that E. faecium was vertically transmitted from the mother hen to the offspring, as E. faecium was recovered in 20 and 33% of the offspring pre- and post-hatch, respectively. When applied on the eggshell, the recovery of E. faecium before hatch depended on the application method and ranged from 0 to 9%. In contrast, L. animalis was not recovered before hatch. Moreover, when sampling post-hatch 100% of the chickens were colonised when E. faecium was used and 54% were colonised when L. animalis was used. Furthermore, spray application with E. faecium was the most successful application method as 9% of the foetuses were colonised pre-hatch and 100% became colonised post-hatch. Therefore, pre-hatch application by, for example, spray of probiotics on the eggshell can be used as an easy-to-use, noninvasive method for early life colonisation of chicken gut.

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Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection

Cited by 2 publications

References 32 publications

In ovo inoculation of an Enterococcus faecium–based product to enhance broiler hatchability, live performance, and intestinal morphology

In ovo inoculation of an Enterococcus faecium–based product to enhance broiler hatchability, live performance, and intestinal morphology

Recovery of probiotic bacteria from the intestinal tract of broilers after noninvasive pre-hatch application

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