Post-capture hyperbaric simulations to study the mechanism of shell regeneration of the deep-sea hydrothermal vent mussel Bathymodiolus azoricus (Bivalvia: Mytilidae)

Kádár, Enikõ; Guerra-Tschuschke, Isabel; Checa, António

doi:10.1016/j.jembe.2008.07.028

Cited by 12 publications

(4 citation statements)

References 28 publications

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“…The selected putative genes are being currently investigated under controlled conditions in our laboratory, to assess the effect of long-term acclimatization in aquaria at atmospheric pressure, the effect of de novo hyperbaric stimulations in the IPOCAMP chamber and the effect of exposure to marine Vibrio bacteria, on B. azoricus transcript profiling experiments [ 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

et al. 2010

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BackgroundBathymodiolus azoricus is a deep-sea hydrothermal vent mussel found in association with large faunal communities living in chemosynthetic environments at the bottom of the sea floor near the Azores Islands. Investigation of the exceptional physiological reactions that vent mussels have adopted in their habitat, including responses to environmental microbes, remains a difficult challenge for deep-sea biologists. In an attempt to reveal genes potentially involved in the deep-sea mussel innate immunity we carried out a high-throughput sequence analysis of freshly collected B. azoricus transcriptome using gills tissues as the primary source of immune transcripts given its strategic role in filtering the surrounding waterborne potentially infectious microorganisms. Additionally, a substantial EST data set was produced and from which a comprehensive collection of genes coding for putative proteins was organized in a dedicated database, "DeepSeaVent" the first deep-sea vent animal transcriptome database based on the 454 pyrosequencing technology.ResultsA normalized cDNA library from gills tissue was sequenced in a full 454 GS-FLX run, producing 778,996 sequencing reads. Assembly of the high quality reads resulted in 75,407 contigs of which 3,071 were singletons. A total of 39,425 transcripts were conceptually translated into amino-sequences of which 22,023 matched known proteins in the NCBI non-redundant protein database, 15,839 revealed conserved protein domains through InterPro functional classification and 9,584 were assigned with Gene Ontology terms. Queries conducted within the database enabled the identification of genes putatively involved in immune and inflammatory reactions which had not been previously evidenced in the vent mussel. Their physical counterpart was confirmed by semi-quantitative quantitative Reverse-Transcription-Polymerase Chain Reactions (RT-PCR) and their RNA transcription level by quantitative PCR (qPCR) experiments.ConclusionsWe have established the first tissue transcriptional analysis of a deep-sea hydrothermal vent animal and generated a searchable catalog of genes that provides a direct method of identifying and retrieving vast numbers of novel coding sequences which can be applied in gene expression profiling experiments from a non-conventional model organism. This provides the most comprehensive sequence resource for identifying novel genes currently available for a deep-sea vent organism, in particular, genes putatively involved in immune and inflammatory reactions in vent mussels.The characterization of the B. azoricus transcriptome will facilitate research into biological processes underlying physiological adaptations to hydrothermal vent environments and will provide a basis for expanding our understanding of genes putatively involved in adaptations processes during post-capture long term acclimatization experiments, at "sea-level" conditions, using B. azoricus as a model organism.

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Section: Resultsmentioning

confidence: 99%

High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

et al. 2010

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“…Regeneration or repair of the fractured shell is dependent mostly on secretory contents from the mantle epithelium ( Marin et al, 2008 ) and partly from hemocytes ( Mount et al, 2004 ; Kadar, Tschuschke & Checa, 2008 ). The epithelial cells of the mantle secrete matrix proteins, transport various ions through ion channels, and form a supersaturated environment in the extrapallial space for shell biomineralization ( Mann, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Calcineurin subunit B is involved in shell regeneration inHaliotis diversicolor

Buddawong

Asuvapongpatana

Suwannasing

et al. 2021

PeerJ

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Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.

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“…Artificially induced repair processes have been studied in a number of cases, after shell cutting (Caseiro and Gauthier, 1997) or shell drilling (Kapur and Sen Gupta, 1970;Caseiro and Gauthier, 1998;Fleury et al, 2008;Kadar et al, 2008). In natural environments, the shell repair process frequently takes place after accidental shell breakage, shell boring by epibionts (Marshall and Day, 2001;Nollens et al, 2002;Huchette et al, 2006), or predation by fishes.…”

Section: Introductionmentioning

confidence: 99%

Variability of shell repair in the Manila clam Ruditapes philippinarum affected by the Brown Ring Disease: A microstructural and biochemical study

Trinkler¹,

Guichard²,

Labonne³

et al. 2011

Journal of Invertebrate Pathology

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Post-capture hyperbaric simulations to study the mechanism of shell regeneration of the deep-sea hydrothermal vent mussel Bathymodiolus azoricus (Bivalvia: Mytilidae)

Cited by 12 publications

References 28 publications

High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

Calcineurin subunit B is involved in shell regeneration inHaliotis diversicolor

Variability of shell repair in the Manila clam Ruditapes philippinarum affected by the Brown Ring Disease: A microstructural and biochemical study

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