Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage

Abstract

Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment.

Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State

“…Some ECM molecules reported to modulate MSC differentiation are also observed in MSC spheroids. Upregulation of collagen V, 99 laminin, 100 and perlecan 101 was seen in MSC spheroids; in 2D studies, these ECM molecules respectively were seen to promote chondrogenesis, neurite outgrowth, and osteogenesis while blocking adipogenesis. Interestingly, collagen IV, which in 2D studies is upregulated during adipogenic induction, is less expressed in 3D MSC spheroid cultures.…”

Advances in Mechanical Properties of Hydrogels for Cartilage Tissue Defect Repair

“…Therefore, tissue adhesion should also be considered. In addition, Cruz et al [44] found that Collagen V stimulated adipose stem cells induced a significant increase in Col II in areas of osteoarthritic articular cartilage degeneration, improved cell distribution and actin fiber structure during the initial stages of chondroblast differentiation, and promoted adhesion. Understanding the mechanisms by which new tissues adhere to native cartilage is crucial, making biomaterials more likely to be used to treat osteoarthritic lesions.…”

A novel cell source for therapy of knee osteoarthritis using atelocollagen microsphere-adhered adipose-derived stem cells: Impact of synovial fluid exposure on cell activity

“…To enhance the therapeutic efficacy of ADSCs against knee OA, it is essential to maintain the viability of ADSCs within the joint cavity. Various methods have been developed to prolong the survival of stem cells at the site of administration, including the use of spheroid culture techniques [ 12 , 13 ] and improvement of the culture medium [ 14 , 15 ]. Among these methods, microspheres have recently emerged as a highly promising culture tool [ 16 , 17 ].…”