Possible Sodium and D-Glucose Cotransport in Isolated Jejunal Epithelium of Children

Grasset, E.; Heyman, Martine; Dumontier, Anne Marie; Lestradet, H; Desjeux, J. F.

doi:10.1203/00006450-197911000-00008

Cited by 16 publications

(12 citation statements)

References 10 publications

(27 reference statements)

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“…The electrical parameters (Table 3) obtained in control biopsies were similar to those obtained previously in children recovering from malnutrition (8), and in healthy children previously studied (19). The 95% confidence interval for Isc was 2 1.5 to 39.05 PA. ~m -~.…”

Section: Resultssupporting

confidence: 67%

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Grasset²,

et al. 1988

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ABSTRACT. To establish if intestinal permeability to exogenous antigens is involved in cow's milk allergy (CMA) in infants, 33 children 1 to 24 months old (18 controls and 1 5 with CMA) were tested for intestinal permeability to the protein marker horseradish peroxidase (HRP). Jejunal biopsies were performed either during the initial period of diagnosis, at the mean (and SE) age of 3 f 1 months, and/or 1 yr later, at the age of 13 f 2 months, just before and after a milk challenge. A small fragment of the biopsy was studied for histology and the remainder was mounted in an Ussing chamber for simultaneous measurement of mucosal to serosal transport of H R P in its intact and degraded forms and electrical parameters including short-circuit current and conductance. No modification in H R P absorption was noted in control children aged from 2 months to 11 yr, indicating that gut closure probably occurred earlier in life. During the initial period of CMA, transepithelial H R P fluxes were significantly higher, about 8-fold, in children with CMA (intact H R P flux = 48.5 f 15.2, 95% confidence interval, 11.2 to 85.7 versus 5.9 + 1.2, 95% confidence interval 2.9 to 8.3, in control children) In addition, short-circuit current was increased but conductance was unchanged. After several months on a milkfree diet, H R P flux and short-circuit current returned to control values. Just after the milk challenge and independently of -the clinical issue, a slight rise in H R P permeability was observed but it was not significant and remained within control values. These results suggest that increased permeability to proteins is probably not the primary cause of CMA, and that the increase in protein endocytosis noted during the initial period of CMA seems rather to be a secondary effect of an abnormal immunological response leading to mucosal inflammation and impairment of the endocytic process. (Pediatr Res 24:197-202, 1988)

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Section: Resultssupporting

confidence: 67%

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Grasset²,

et al. 1988

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“…The calculated EC 50 for D ‐glucose, 7 m M , was considerably smaller than the EC 50 of 21–58 m M found in a perfusion study in the proximal duodenum (Sparsø et al . 1984), whilst more similar to Ussing chamber measurements with EC 50 of 8 m M in the human jejunum (Grasset et al . 1979).…”

Section: Discussionsupporting

confidence: 71%

Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies

Larsen¹,

Nielsen²,

Hansen³

et al. 2001

Acta Physiologica Scandinavica

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The aim of this study was to design and evaluate a modified Ussing chamber, that makes use of constant air suction (modified Ussing air suction chamber, MUAS) for fixation of biopsy specimens. Standard size forceps biopsies were taken from the descending part of duodenum from patients undergoing endoscopy. Short circuit current (SCC) and conductance (G) were measured during basal conditions and after addition of different sugars and secretagogues. Histologic examination was performed to determine the degree of tissue damage after study in the chamber. Basal SCC was 54.7 +/- 4.3 microA x cm(-2) and G was 58.7 +/- 4.7 mS x cm(-2) (mean +/- SEM, n=48) and steady values of these parameters were observed for at least 2 h. Reproducible and steady responses in SCC were obtained with D-glucose (SCCmax=172 +/- 22.1 microA x cm(-2); EC50=6.9 +/- 0.7 mM, n=5) and D-galactose (SCCmax=233 +/- 55.7 microA x cm(-2); EC50=9.2 +/- 0.7 mM, n=3), and secretory responses with 5-hydroxytryptamine, 100 microM (DeltaSCC= 16.1 +/- 3.8 microA x cm(-2), n=10), histamine, 100 microM (DeltaSCC=24.0 +/- 4.1 microA x cm(-2), n=10) and prostaglandin E2, 1 microM (DeltaSCC=30.3 +/- 5.4 microA x cm(-2), n=6). Experimental biopsy specimens showed intact surface epithelium by histologic examination and did not differ from controls apart from minor indications of edge damage. No difference in basal electrical parameters and D-glucose fluxes were found between Helicobacter pylori positive and negative patients. Our data suggests that the MUAS chamber represents a promising alternative approach to measure transport processes in intestinal endoscopic biopsies.

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“…The biopsy specimiien was mounted between two lucite chambers as a flat sheet on filter paper (22). The exposed area was 12.57 mm2.…”

Section: Methodsmentioning

confidence: 99%

“…These coincentrations were measured as previously described (14) using pieces of jejunal epithelium and the immediately underlying tissue without the muscular layer (22). In brief, the tissue was shaken at 37°C for 60 min in 10 ml of Ringer or Na-free solution containing 3 Lysinle inlflux.…”

Section: Methodsmentioning

confidence: 99%

Lysine Fluxes across the Jejunal Epithelium in Lysinuric Protein Intolerance

Desjeux¹,

Rajantie²,

Simell³

et al. 1980

J. Clin. Invest.

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Possible Sodium and D-Glucose Cotransport in Isolated Jejunal Epithelium of Children

Cited by 16 publications

References 10 publications

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies

Lysine Fluxes across the Jejunal Epithelium in Lysinuric Protein Intolerance

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