Possible selection of viable human blastocysts after vitrification by monitoring morphological changes

Maezawa, Tadashi; Yamanaka, M.; Hashimoto, Shu; Amo, A.; Ohgaki, A.; Nakaoka, Yoshiharu; Fukuda, Aisaku; Ikeda, Tomoaki; Inoue, Masayasu; Morimoto, Yoshiharu

doi:10.1007/s10815-014-0260-x

Cited by 21 publications

(19 citation statements)

References 17 publications

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“…This is globally in accord with previous reports that used comparable culture systems without cells (20–31%, Shirazi et al, ; 0–29%, Schmaltz‐Panneau et al, ; 41 ± 4.5%, Lopera‐Vasquez et al, ; 48 ± 9%, Lopera‐Vasquez et al, ). However, embryo re‐expansion may be difficult to evaluate because, over time after warming, blastocysts often show repeated collapse and re‐expansion (Maezawa et al, ; Niimura et al, ). Moreover, the duration of the re‐expansion process, if any, is highly variable among blastocysts (Ebner et al, ; Niimura et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Intraoviductal concentrations of steroid hormones during in vitro culture changed phospholipid profiles and cryotolerance of bovine embryos

Banliat

Dubuisson

Corbin

et al. 2019

Molecular Reproduction Devel

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The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented with 55 ng/ml P4, 120 pg/ml E2, 40 ng/ml CO, or their combination (ALL). Control embryos were cultured with vehicle (0.1% ethanol). Exposure to steroids did not affect the embryo developmental rate nor the mean number of cells per blastocyst. However, at 24 hr after vitrification‐warming, exposure to P4 improved the proportion of embryos that re‐expanded and were viable while exposure to CO decreased the proportion of viable embryos. By intact cell MALDI‐TOF mass spectrometry, a total of 242 phospholipid masses of 400–1000 m/z were detected from individual fresh blastocysts. Exposure to ALL induced the highest and most specific changes in embryo phospholipids, followed by P4, E2, and CO. In particular, the m/z 546.3 and 546.4 attributed to lysophosphatidylcholines were found less abundant after exposure to P4. In conclusion, exposure of bovine embryos to intraoviductal concentrations of steroid hormones did not affect in vitro development but changed blastocyst quality in terms of cryotolerance and phospholipid profiles.

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Section: Discussionmentioning

confidence: 99%

“…T A B L E 2 Developmental rates of bovine blastocysts exposed in vitro to to intraoviductal concentrations of progesterone, estradiol, cortisol, or a combination of all at Day 8 (Maezawa et al, 2014;Niimura et al, 2010). Moreover, the duration of the re-expansion process, if any, is highly variable among blastocysts (Ebner et al, 2017;Niimura et al, 2010).…”

mentioning

confidence: 99%

Intraoviductal concentrations of steroid hormones during in vitro culture changed phospholipid profiles and cryotolerance of bovine embryos

Banliat

Dubuisson

Corbin

et al. 2019

Molecular Reproduction Devel

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“…In terms of embryo scoring, morphokinetics has taken over the leading role from pure morphological evaluation and it was only a matter of time before time-lapse data dealing with vitrified/warmed blastocysts were published [12]. This report roughly classified vitrified/warmed blastocysts by whether they had shrunk or not at 5 to 6 h after warming and then retrospectively screened the video sequences for evidence of temporary re-expansion [12].…”

Section: Introductionmentioning

confidence: 99%

“…This report roughly classified vitrified/warmed blastocysts by whether they had shrunk or not at 5 to 6 h after warming and then retrospectively screened the video sequences for evidence of temporary re-expansion [12].…”

Section: Introductionmentioning

confidence: 99%

“…Since in this preliminary study [12] the time-lapse incubator was not primarily used to measure the exact timing of particular events of the warming process, the present study was set up to generate more robust data on early morphokinetic variables of warmed blastocysts. By doing so, we aimed for a decision aid of clinical relevance, e.g., determining whether to transfer a warmed blastocyst or to warm an additional one on time.…”

Section: Introductionmentioning

confidence: 99%

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Morphokinetics of vitrified and warmed blastocysts predicts implantation potential

Ebner

Oppelt

Radler

et al. 2016

J Assist Reprod Genet

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Purpose It was studied whether morphokinetics of blastocoele re-expansion and hatching in vitrified-warmed blastocysts is predictive of implantation, clinical pregnancy, and live birth. Methods In 144 patients aiming for single warmed blastocyst transfer, blastocysts were cultured in a new time-lapse system (Miri® TL) immediately after warming. Video sequences with an image interval of 5 min were annotated and the corresponding morphokinetic variables were correlated with pregnancy outcome. In detail, tRE (start of re-expansion), tCRE (completion of re-expansion), tAH (hatching from the manipulated zona pellucida), and presence of collapses were recorded. Results In the pregnant group, tRE and tCRE were significantly lower (0.69 ± 0.45 h and 2.16 ± 0.94 h) as compared to the non-pregnant group (1.23 ± 1.08 h and 2.70 ± 1.20 h). Both variables and the duration of re-expansion (tCRE-tRE) allowed for distinction between Bnon-pregnant,^Bloss of pregnancy,^and live birth/ongoing pregnancy. Presence and number of collapses showed no correlation with outcome. Conclusions Time-lapse imaging of vitrified-warmed blastocysts offers additional selection criteria allowing for prediction of implantation potential. As a consequence, cumulative pregnancy rate could be increased and time-to-pregnancy reduced.

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Mitochondrial function of human embryo: Decline in their quality with maternal aging

Hashimoto

Morimoto

2022

Reprod Medicine & Biology

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Women's fertility declines with age. 1 One of the main causes of low development of embryos obtained from older women is the rising proportion of chromosome abnormalities 2 provoked by premature separation of bivalent to univalent during meiosis. 3 Furthermore, age-related fall in mitochondrial function of oocytes has also been suggested as a cause of low embryonic developmental potential. [4][5][6][7] Mitochondria produce ATP for cellular activity by oxidative phosphorylation. The tricarboxylic acid (TCA) cycle is characterized by a higher capacity to produce ATP per mole of substrate than the anaerobic glycolysis pathway. The ATP produced by mitochondria

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Possible selection of viable human blastocysts after vitrification by monitoring morphological changes

Abstract: Data of the present study suggest a group of shrunken blastocysts contains many viable and clinically available embryos and time-lapse observation of vitrified-warmed blastocysts is a potential method to distinguish viable embryos from shrunken blastocysts.

Cited by 21 publications

References 17 publications

Intraoviductal concentrations of steroid hormones during in vitro culture changed phospholipid profiles and cryotolerance of bovine embryos

Intraoviductal concentrations of steroid hormones during in vitro culture changed phospholipid profiles and cryotolerance of bovine embryos

Morphokinetics of vitrified and warmed blastocysts predicts implantation potential

Mitochondrial function of human embryo: Decline in their quality with maternal aging

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