Possible roles of parathyroid hormone, 1.25(OH)2D3, and fibroblast growth factor 23 on genes controlling calcium metabolism across different tissues of the laying hen

Gloux, Audrey; Roy, Nathalie Le; Ezagal, Jacky; Même, Nathalie; Hennequet-Antier, Christelle; Piketty, M.L.; Prié, Dominique; Benzoni, G; Gautron, Joël; Nys, Yves; Narcy, Agnès; Duclos, M. J.

doi:10.1016/j.domaniend.2019.106407

Cited by 13 publications

(27 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All animals had free access to water and feed, and were subjected to a photoperiodic program of 14 h light:10 h dark. Six or seven laying hens per diet and post-ovulation stage (0-1 h, 9-10 h and 18-19 h PO) were submitted to blood collection and subsequently euthanized as described before 18 . Tissue samples (jejunum, medullary bone of the left tibia, left kidney, parathyroid gland and a piece of liver) were collected and stored at − 80 °C until further analyses.…”

Section: Resultsmentioning

confidence: 99%

“…4a-c). The expression of the Ca-binding protein CALB1 was higher at 0-1 than at 9-10 h PO, and intermediate at [18][19] h PO (Fig. 4d).…”

Section: Expression Of Genes Involved In Renal Reabsorption or Jejunamentioning

confidence: 90%

“…www.nature.com/scientificreports/ in the bone structure have been reported, the molecular mechanisms involved have not been studied so far. We recently identified the coordinated response of candidate genes regulating Ca homeostasis across tissues in young laying hens compared at different stages of the ovulatory cycle with minimal, intermediate or high mineralizing activity 18 . The high demand of Ca by the uterus for eggshell formation activates the Parathyroid Hormone (PTH) gene and the genes under its control involved in medullary bone resorption.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Increased expression of fibroblast growth factor 23 is the signature of a deteriorated Ca/P balance in ageing laying hens

Gloux

Roy

Même

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

The present study concerned the effect of ageing in laying hens, from 23 to 90 weeks of age, on the regulation of Ca metabolism related to the requirement for eggshell mineralization. Samples were collected from parathyroid gland (PG), liver, jejunum, medullary bone (MB) and kidney for a quantitative study of candidate gene expression. Although parathyroid hormone (PTH) gene expression in the PG did not vary with age, a stronger challenge to Ca homeostasis was suggested in aged hens. Indeed gene expression of Ca transporters , Vitamin D Receptor (VDR) in the jejunum, and that of transient receptor potential channel subfamily V member 5 (TRPV5) in the kidney decreased. This could exacerbate bone resorption and impair bone accretion, as attested by a higher expression of the Carbonic Anhydrase 2 (CA2) gene and a lower expression of collagen type I alpha 1 chain (COL1A1) in the MB. The increased expression of Fibroblast Growth Factor 23 (FGF23) in the MB likely contributed to the decreased plasma levels of 1.25(OH)2D3 and the altered expression of target genes under its regulation. Our data highlights the molecular mechanisms underlying the osteoporotic syndrome previously documented in aged laying hens, thus providing new perspectives for future interventions.

show abstract

Section: Resultsmentioning

confidence: 99%

“…4a-c). The expression of the Ca-binding protein CALB1 was higher at 0-1 than at 9-10 h PO, and intermediate at [18][19] h PO (Fig. 4d).…”

Section: Expression Of Genes Involved In Renal Reabsorption or Jejunamentioning

confidence: 90%

mentioning

confidence: 99%

See 1 more Smart Citation

Increased expression of fibroblast growth factor 23 is the signature of a deteriorated Ca/P balance in ageing laying hens

Gloux

Roy

Même

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to parathyroid hormone, and 1,25 dihydroxy vitamin D 3 (Castillo et al, 1979), there appears to be an involvement of other mechanisms. There is increased expression of receptor activator of nuclear factor-kB (RANK) and fibroblast growth factor (FGF23) in medullary bone of hens peaking at the end of egg calcification and the end of calcium mobilization from the medullary bone (Gloux et al, 2020). There was also increased expression of solute carrier family 20 member 1 (SLC20A1) and member 2 (SLC20A2) (Gloux et al, 2020).…”

Section: Female Reproductionmentioning

confidence: 99%

“…There is increased expression of receptor activator of nuclear factor-kB (RANK) and fibroblast growth factor (FGF23) in medullary bone of hens peaking at the end of egg calcification and the end of calcium mobilization from the medullary bone (Gloux et al, 2020). There was also increased expression of solute carrier family 20 member 1 (SLC20A1) and member 2 (SLC20A2) (Gloux et al, 2020). Moreover, circulating concentrations of phosphate are elevated in laying hens passively immunized against FGF23 (Ren et al, 2019).…”

Section: Female Reproductionmentioning

confidence: 99%