Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction

Zhang, X.; Kidder, Gerald M.; Watson, Andrew J.; Schultz, Gilbert A.; Armstrong, David T.

doi:10.1530/jrf.0.1000375

Cited by 59 publications

(37 citation statements)

References 13 publications

(14 reference statements)

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“…In vitro experiments suggest the growth and development of the preimplantation embryo is regulated by an array of cytokines and growth factors secreted from epithelial cells lining the oviduct and uterus (16,17). GM-CSF is a cytokine originally identified as a product of activated T-lymphocytes involved in the proliferation and differentiation of myeloid hematopoietic cells (18).…”

Section: Discussionmentioning

confidence: 99%

Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis

Behr

Mooney

Wen

et al. 2005

J Assist Reprod Genet

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Purpose : To investigate the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the development of preimplantation mouse embryos. Methods : Mouse 2-cell embryos were collected and cultured in P-1 medium supplemented with GM-CSF at different concentrations. Using reverse transcription-polymerase chain reaction, expression Bcl-2 and Bax mRNA in blastocyst were evaluated in the GM-CSF group and control group. Apoptosis detection was performed using the in situ apoptosis detection kit in mouse blastocysts. The statistical significance of the data was analyzed using t-test and chi-square test. Results : The development of blastocyst increased to 89% in the addition of GM-CSF (0.125 ng/mL), compared to controlled group (80%). The number of cells staining for apoptosis was lower in GM-CSF group than that in the control group. Bcl-2 expression was found to be upregulated in blastocysts in the GM-CSF supplemented group compared to the control group. Conclusion : These results suggest that GM-CSF might be an important regulator in embryo development.KEY WORDS: Apoptosis; blastocyst development; GM-CSF; in vivo blastocyst.

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Section: Discussionmentioning

confidence: 99%

Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis

Behr

Mooney

Wen

et al. 2005

J Assist Reprod Genet

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“…The normal functioning of TGF b-1 is important for normal implantation (Das et al 1997) and it is an essential growth factor for the normal functioning of the endometrium (Polli et al 1996). Similarly, normal IGF-I expression is essential for both normal uterine function and embryonic development (Gu et al 1999, Zhang et al 1994). …”

Section: Discussionmentioning

confidence: 99%

“…IGF immunoreactivity is present in all animal tissues, particularly the uterus and trophoblast (AlvarezRodriguez & Baiza-Guzman 1996). Both IGF-I and IGF-II are essential for early embryo and uterine development (Gu et al 1999, Zhang et al 1994). …”

Section: Introductionmentioning

confidence: 99%

Endometriotic Cells Exhibit Metaplastic Change and Oxidative DNA Damage as Well as Decreased Function, Compared to Normal Endometrium

et al. 2005

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A widely accepted theory of the etiology of endometriosis is that it originates from the implantation and invasion of cells from retrograde menstruation to various sites in the body particularly the pelvic peritoneal cavity. Little is known of the function of these cells in ectopic sites. Normal endometrium was compared with endometriotic tissue using an antibody to Placental Cadherin (P Cadherin), a recently studied cadherin that is implicated in metaplasia and early neoplasia and also 8-hydroxyguanine, an indicator of oxidative DNA damage. Comparisons of endometrial tissue function were made using expression of transforming growth factor beta-1 (TGFbeta-1) and insulin-like growth factor-I (IGF-I). There was no labelling for anti-P Cadherin or anti-8-hydroxydeoxyguanosine in normal endometrium but marked labelling for both on the apical surface of the endometriotic epithelium. Studies of markers of normal endometrial function were all de-expressed in endometriosis. This study indicates that endometriosis cells are abnormal and exhibit oxidative DNA damage, metaplasia and markedly reduced function compared to normal endometrium.

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“…IGFI binds solely to the IGFIR, while IGFII binds strongly to the IGFIIR and weakly to the IGFIR. IGFI and IFG-II are expressed in the oviduct (Dalton et al 1994;Lighten et al 1998;Pfeifer and Chegini 1994;Carlsson et al 1993;Shao et al 2007;Zhang et al 1994). IGFI added to mouse embryos in vitro can promote pre-implantation embryo development, cell number and metabolism (Brison and Schultz 1997).…”

Section: Insulin-like Growth Factor I and Ii (Igfi And Igfii)mentioning

confidence: 99%

Female Tract Cytokines and Developmental Programming in Embryos

Robertson

Chin

Schjenken

et al. 2015

Advances in Experimental Medicine and Biology

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In the physiological situation, cytokines are pivotal mediators of communication between the maternal tract and the embryo. Compelling evidence shows that cytokines emanating from the oviduct and uterus confer a sophisticated mechanism for 'fine-tuning' of embryo development, influencing a range of cellular events from cell survival and metabolism, through division and differentiation, and potentially exerting long-term impact through epigenetic remodelling. The balance between survival agents, including GM-CSF, CSF1, LIF, HB-EGF and IGFII, against apoptosis-inducing factors such as TNFα, TRAIL and IFNg, influence the course of preimplantation development, causing embryos to develop normally, adapt to varying maternal environments, or in some cases to arrest and undergo demise. Maternal cytokine-mediated pathways help mediate the biological effects of embryo programming, embryo plasticity and adaptation, and maternal tract quality control. Thus maternal cytokines exert influence not only on fertility and pregnancy progression but on the developmental trajectory and health of offspring. Defining a clear understanding of the biology of cytokine networks influencing the embryo is essential to support optimal outcomes in natural and assisted conception.

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Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction

Cited by 59 publications

References 13 publications

Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis

Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis

Endometriotic Cells Exhibit Metaplastic Change and Oxidative DNA Damage as Well as Decreased Function, Compared to Normal Endometrium

Female Tract Cytokines and Developmental Programming in Embryos

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