Possible role of WNT10B in increased proliferation and tubule formation of human umbilical vein endothelial cell cultures treated with hypoxic conditioned medium from human adipocytes

Pourdashti, Sara; Faridi, Nassim; Yaghooti, Hamid; Jalali, Mohammad Taha; Soroush, Ahmadreza; Bathaie, S. Zahra

doi:10.1080/10520295.2021.1923801

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…WNT10B regulates VEGFA in umbilical cord mesenchymal stem cells ( Liu et al, 2020g ) and vascular endothelial growth factor receptor 2 ( VEGFR2 ) in endothelial cells (( Paik et al, 2015 ). Furthermore, the expression of WNT10B and VEGFA genes correlate in the subcutaneous white adipose tissue of 80 individuals, and hypoxia increases WNT10B and VEGFA in adipocytes ( Pourdashti et al, 2022 ).…”

Section: Wnt10b and Endothelial Cells/angiogenesismentioning

confidence: 99%

The role of WNT10B in physiology and disease: A 10-year update

Perkins

Singh

Abell

et al. 2023

Front. Cell Dev. Biol.

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WNT10B, a member of the WNT family of secreted glycoproteins, activates the WNT/β-catenin signaling cascade to control proliferation, stemness, pluripotency, and cell fate decisions. WNT10B plays roles in many tissues, including bone, adipocytes, skin, hair, muscle, placenta, and the immune system. Aberrant WNT10B signaling leads to several diseases, such as osteoporosis, obesity, split-hand/foot malformation (SHFM), fibrosis, dental anomalies, and cancer. We reviewed WNT10B a decade ago, and here we provide a comprehensive update to the field. Novel research on WNT10B has expanded to many more tissues and diseases. WNT10B polymorphisms and mutations correlate with many phenotypes, including bone mineral density, obesity, pig litter size, dog elbow dysplasia, and cow body size. In addition, the field has focused on the regulation of WNT10B using upstream mediators, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). We also discussed the therapeutic implications of WNT10B regulation. In summary, research conducted during 2012–2022 revealed several new, diverse functions in the role of WNT10B in physiology and disease.

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Section: Wnt10b and Endothelial Cells/angiogenesismentioning

confidence: 99%

The role of WNT10B in physiology and disease: A 10-year update

Perkins

Singh

Abell

et al. 2023

Front. Cell Dev. Biol.

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“…The research shows that in HUVECs, Wnt10b functions as an angiogenesis-related gene and may help control blood vessel growth and development and promote angiogenesis. 46 Therefore, the expression level of Wnt10b was increased not only in TK and TKH spheroids but also in DKH spheroids, and CD31 expression was observed in DKH and TKH spheroids.…”

Section: Discussionmentioning

confidence: 87%

Optimization of hair follicle spheroids for hair-on-a-chip

Jeong,

Nam,

Sung

2024

Biomater. Sci.

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“…In addition, our previous study highlighted the critical role of WNT10B in adipose tissue angiogenesis. As a positive regulator, it has been shown that WNT10B promotes the formation of new vessels through the upregulation of VEGFA expression [35].…”

Section: Discussionmentioning

confidence: 99%

Subcutaneous adipocyte size but not adiposity was associated with inflammation, ER stress, and insulin resistance markers

Pourdashti

Faridi

Monem-Homaie

et al. 2023

Preprint

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Background: The fat storage capability of the adipose tissue prevents ectopic lipid deposition, which is one of the risk factors for metabolic abnormalities in obesity. This capability depends on the adipogenic gene expression and blood supply provision for tissue expansion through angiogenesis. Here, we studied hyperplasia/hypertrophy of subcutaneous white adipose tissue (scWAT) concerning adipogenic gene expression, angiogenic status, and metabolic parameters in non-obese, Class I, and Class II+III obese subjects. Methods: The scWAT samples were collected from 80 subjects. The anthropometric parameters, adipose tissue cell size, serum biochemistry, ER stress induced XBP1 splicing, PPARγ2, SFRP1, WNT10B, and VEGFA gene expression levels were studied. In addition, the CD31 level was investigated by Western blotting. Results: The obese subjects had greater waist circumferences and higher serum TG, TC, insulin, and HOMA-IR than the non-obese group. The Class I obese group showed the largest adipocyte size, increased TNFα, insulin, HOMA-IR, and sXBP-1, WNT10B, and VEGFAexpression. In contrast, the expression of SFRP1 was not significantly different between all studied groups. The Class II+III obesity group showed high PPARγ2 expression and CD31 levels. Class I obesity, with hypertrophic scWAT adipocytes and limited capability of adipose tissue expansion, showed inflammation, insulin resistance, and ER stress. Conclusion: The results suggest that the capability of adipogenesis with inadequate angiogenesis is related to metabolic status, inflammation, and ER function. Therefore, therapeutic strategies to simultaneously promote angiogenesis and adipogenesis can effectively prevent obesity complications.

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Possible role of WNT10B in increased proliferation and tubule formation of human umbilical vein endothelial cell cultures treated with hypoxic conditioned medium from human adipocytes

Cited by 4 publications

References 32 publications

The role of WNT10B in physiology and disease: A 10-year update

The role of WNT10B in physiology and disease: A 10-year update

Optimization of hair follicle spheroids for hair-on-a-chip

Subcutaneous adipocyte size but not adiposity was associated with inflammation, ER stress, and insulin resistance markers

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