Possible role of the cytosol pathway of acetyl-CoA supply in terpene biosynthesis in sweet potato infected with Ceratocystis fimbriata1

Takeuchi, Akira; Scott, K. J.; Oba, Kazuko; Uritani, Ikuzô

doi:10.1093/oxfordjournals.pcp.a076065

Cited by 7 publications

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“…For example, the sesquiterpene ipomeamarone is produced from farnesyl diphosphate in sweet potato infected by C. fimbriata , and bicyclic diterpene casbene from geranylgeranyl diphosphate by caster bean ( Ricinus communis ) seedling is greatly stimulated following infection with Rhizopus stolonifer [3,4]. It has been reported that pyruvate is a more effective precursor for sesquiterpene biosynthesis than citrate, and a cytosolic pathway which utilizes the enzymes pyruvate decarboxylase, aldehyde dehydrogenase and acetyl‐CoA synthetase, may play an important role in supply of acetyl‐CoA for sesquiterpene biosynthesis [5]. However, there is now a need to further analyze whether ACL1 might be involved in the production of acetyl‐CoA required for the formation of phytoalexins after pathogen infection in the hot pepper.…”

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confidence: 99%

Pathogen‐induced expression of plant ATP:citrate lyase¹

Suh

Lee

et al. 2001

FEBS Letters

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Hypersensitive response (HR) in plants is generally characterized by a rapid and localized cell death, cell wall strengthening and synthesis of phytoalexins and PR proteins [1]. In order to understand the molecular and cellular defense mechanisms better during HR that results from the interaction between plant pathogen and its non-host, we inoculated hot pepper (Capsicum annuum cv. Pukang) leaves with soybean pustule pathogen, Xanthomonas campestris pv. glycines8ra. A pool of genes induced or repressed by infection of the pathogen has been isolated by using a di¡erential displaypolymerase chain reaction (DD-PCR) technique (Yi et al., unpublished data). Approximately one half of the isolated genes had no sequence similarity in existing databases and the major portion of identi¢ed DNA fragments consisted of enzymes involved in primary or secondary metabolic pathways. One of the fragments was found to have signi¢cant sequence homology with ATP:citrate lyase (ACL) known from diverse organisms including alga, fungi, and mammals.ACL is known to catalyze the following reaction: citrate+ CoA+ATPCoxaloacetate+ADP+Pi+acetyl-CoA. In the cytosol of animals, oleaginous yeast, and fungi, acetyl-CoA produced by the action of ACL is the major precursor for fatty acid and sterol biosynthesis [2]. However, in plants, the biosynthetic origin of cytosolic acetyl-CoA that is needed for biosynthesis of phytochemicals is not established. In sweet potato, ACL activity has been observed to increase 10-fold in root tissues response to infection by the fungal pathogen, Ceratocystis ¢mbriata, and to correspond to the accumulation of phytoalexin ipomeamarone [3], but the molecular characterization of a plant ACL has not yet been reported.In order to isolate a full-length cDNA clone encoding ACL, a cDNA library was constructed from hot pepper leaves inoculated with X. campestris pv. glycines8ra. After screening of the cDNA library by using a cDNA fragment of ACL produced by DD-PCR as a probe, a cDNA clone with an open reading frame containing 608 amino acids was ¢nally isolated and designated as Ca-ACL1 (GenBank accession number: AF290958). The deduced amino acid of Ca-ACL1 gene has signi¢cant sequence homology with the C-terminal part of known animal ACLs. Calculated molecular mass of hot pepper ACL polypeptide was 66 kDa, which is only half of the animal ACLs. The ACL catalytic center, GHAGA, and the histidine residue that is autophosphorylated by ATP during catalysis are highly conserved in Ca-ACL1, but none of the three additional phosphorylation sites (Tyr446, Ser450, Ser454) that are involved in the regulation of rat ACL activity exists [2].To investigate the expression kinetics of the Ca-ACL1 transcripts during inoculation with X. campestris pv. glycines8ra, total RNA was isolated from hot pepper leaves 0, 4, 8, and 12 h after inoculation, and Northern blot analysis was performed by using 32 P-labeled Ca-ACL1 as a probe. HR cell death on pepper leaves appeared at approximately 15 h post-in¢ltra-tion, whereas no visible symptom was de...

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confidence: 99%

Pathogen‐induced expression of plant ATP:citrate lyase¹

Suh

Lee

et al. 2001

FEBS Letters

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“…Cytosolic acetyl CoA can be synthesized not only from pyruvate via mitochondria but also from acetaldehyde in higher plants. Diseased root tissues of sweet potato produce acetyl CoA for terpene biosynthesis, mainly from pyruvate via acetaldehyde (6). Low-oxygen storage of fruits induces the accumulation of acetaldehyde by pyruvate decarboxylase and a great amount of ethanol production as a result of anaerobic respiration (18).…”

Section: Effects Of Various Oxygen Concentrations On Acetatementioning

confidence: 99%

“…Murphy and Stumpf (4) and Liedvogel and Stumpf (5) demonstrated that free acetate derived from pyruvate via acetyl CoA in the mitochondria moves rapidly to the chloroplast, in which this acetate is utilized to form acetyl CoA in spinach leaf cell. Some acetyl CoA is produced from pyruvate via acetaldehyde and then used for terpene biosynthesis in the diseased tissues of sweet potato (6). Bartley et al (7) reported that acetate esters, such as pentyl and propyl acetates, are formed from fatty acids via β-oxidation of added methyl heptanoate in apples.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Acetate Ester Biosynthesis in Banana (Musa sapientum L.) Fruit Pulp under Anaerobic Conditions

Wendakoon

Ueda

Imahori

et al. 2004

J. Agric. Food Chem.

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The effect of anaerobic conditions on acetate ester biosynthesis in ripened banana pulp was investigated. Incubation of the pulp in less than 1% O(2) resulted in a significant reduction in the formation of ethyl acetate. Regardless of the presence of a large amount of endogenous ethanol and the remaining exogenous isobutyl alcohol after complete anaerobic incubation with the pulp, the production of acetate ester decreased. The effect of addition of pyruvate, isobutyl alcohol, acetate, and methyl hexanoate on acetate ester formation in 100% N(2) was also investigated. The addition of pyruvate and isobutyl alcohol to the pulp gave lower acetate esters in N(2) than in air, whereas the pulp incubated with acetate and isobutyl alcohol produced more acetate ester in both conditions. Therefore, the lack of acetyl CoA, or more precisely acetate, in the tissue is the main reason for the inhibition of acetate ester formation under anaerobic conditions. The activity of beta-oxidation measured by incubation with methyl hexanoate was detected only in the samples incubated in air. The formation of acetyl CoA, derived from pyruvate through mitochondria and through beta-oxidation, was inhibited by anaerobic conditions, which suggests that mitochondrial activity and/or beta-oxidation are essential for ester biosynthesis.

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“…ACL activity and/or cytosolic acetyl-CoA derived products are implicated as essential elements in the response to stress (Takeuchi et al, 1980;Schmid et al, 1990;and Suh et al, 2001) in addition to growth and development. To understand the significance of ACLderived acetyl-CoA to whole plant metabolism, antisense ACLA-1 Arabidopsis plants (herein referred to as anti-ACLA plants) reduced in ATP citrate lyase activity were generated and characterized.…”

Section: (2002)mentioning

confidence: 99%

ATP-citrate lyase has an essential role in cytosolic acetyl-CoA production in Arabidopsis

Fatland

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Possible role of the cytosol pathway of acetyl-CoA supply in terpene biosynthesis in sweet potato infected with Ceratocystis fimbriata1

Cited by 7 publications

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Pathogen‐induced expression of plant ATP:citrate lyase¹

Pathogen‐induced expression of plant ATP:citrate lyase¹

Inhibition of Acetate Ester Biosynthesis in Banana (Musa sapientum L.) Fruit Pulp under Anaerobic Conditions

ATP-citrate lyase has an essential role in cytosolic acetyl-CoA production in Arabidopsis

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Possible role of the cytosol pathway of acetyl-CoA supply in terpene biosynthesis in sweet potato infected with Ceratocystis fimbriata1

Cited by 7 publications

References 0 publications

Pathogen‐induced expression of plant ATP:citrate lyase1

Pathogen‐induced expression of plant ATP:citrate lyase1

Inhibition of Acetate Ester Biosynthesis in Banana (Musa sapientum L.) Fruit Pulp under Anaerobic Conditions

ATP-citrate lyase has an essential role in cytosolic acetyl-CoA production in Arabidopsis

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Pathogen‐induced expression of plant ATP:citrate lyase¹

Pathogen‐induced expression of plant ATP:citrate lyase¹