Possible role of the 72,000 dalton DNA-binding protein in regulation of adenovirus type 5 early gene expression

Carter, T H; Blanton, R. A.

doi:10.1128/jvi.25.2.664-674.1978

Cited by 118 publications

(34 citation statements)

References 44 publications

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“…Thus, although several early genes continue to be expressed (8,16,21), the transition from early to late phase results in a major change in the transcriptional program of adenovirus, in which the viral DNA replication seems to play a central role. This observation was further substantiated by experiments in which DNA replication was prevented by treating the infected cells with inhibitors of DNA (1,9,16,22) or protein synthesis (1,7,11,17), or by incubating at nonpermissive temperature cells that are infected with adenovirus mutants thermosensitive for viral DNA replication (2,5,6). In all cases early transcription occurred normally but there was no shift to late gene expression.…”

mentioning

confidence: 93%

Adenovirus DNA template for late transcription is not a replicative intermediate

Brison¹,

Kédinger²,

Chambon³

1979

J Virol

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The relationship between adenovirus replication and late transcription has been investigated using viral replication and transcription complexes isolated from infected HeLa cell nuclei. These two types of complexes extracted from adenovirus type 2-infected cell nuclei did not sediment at the same rate on sucrose gradients. Viral replicative intermediates were quantitatively precipitated by immunoglobulins raised against purified 72,000-dalton DNA-binding protein, whereas viral transcription complexes remained in the supernatant. These results show that late transcription does not occur on active replication complexes or on 72,000-dalton DNA-binding protein-containing replicative intermediates inactive in DNA synthesis. Additional evidence is presented indicating that it is very unlikely that replicative intermediates lacking the 72,000-dalton DNA-binding protein could be the template for late transcription.

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mentioning

confidence: 93%

Adenovirus DNA template for late transcription is not a replicative intermediate

Brison¹,

Kédinger²,

Chambon³

1979

J Virol

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“…Although cycloheximide alters the relative amounts of the E4 RNAs, all species observed are also produced in the absence of this drug (10). Cells infected with a mutant that produces a defective 72,000-dalton (72K) DNAbinding protein (adenovirus-5 ts125) overproduce E4 RNAs due to a failure to suppress E4 transcription (9,32). Thus, the expression of E4 is under the control of at least two other early viral genes.…”

mentioning

confidence: 99%

Expression of adenovirus-2 early region 4: assignment of the early region 4 polypeptides to their respective mRNAs, using in vitro translation

Tigges¹,

Raskas²

1982

J Virol

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Adenovirus-2 early region 4 (E4; map positions 91.3 to 99.1) encodes six 5' and 3' coterminal, differently spliced mRNAs, which are 2.5, 2.1, 1.8, 1.5, 1.2, and 0.8 kilobases (kb) long. Hybridization selection with five cloned viral DNA fragments that hybridize with subsets of E4 mRNAs was used to purify these six mRNAs and a previously unreported 3.0-kb mRNA from virus-infected cells. E4 mRNAs which were purified by hybridization selection with cloned EcoRI fragment C (map positions 89.7 to 100) were also fractionated by size. The purified mRNAs were then translated in rabbit reticulocyte or wheat germ lysate systems. The full complement of E4 mRNAs specified as many as 16 different polypeptides, with molecular weights ranging from 24,000 (24K) to 10K. The most abundant E4 mRNA, which was 2.1 kb long, specified an 11K polypeptide. The 1.5-kb mRNA, which differed from the 2.1-kb mRNA only by deletion of a second intron from the 3' untranslated region, also specified an 11K polypeptide. The second most abundant mRNA, which was 1.8 kb long, and the 1.2-kb mRNA, which had an intron deleted from the 3' untranslated region, specified a 15K polypeptide. This polypeptide was labeled more intensely with [5,6_3H]leucine than with [35S]methionine. The 3.0and 2.5-kb mRNAs specified four polypeptides (24K, 22K, 19K, and 17K). Translation of E4 mRNAs with a mean size of 0.8 kb, which accumulated preferentially in the presence of cycloheximide, yielded at least 10 polypeptides that migrated in polyacrylamide gels with apparent molecular weights ranging from 21,800 to 10,000. On the basis of translation in wheat germ lysates and the distribution of polypeptides encoded by size-fractionated mRNAs, we concluded that the 0.8-kb mRNA size class includes a heterogeneous mixture of mRNAs which are probably formed as the result of utilization of alternate splice acceptor and donor sites during removal of the second intron. Our polypeptide assignments for the 2.1-, 1.8-, 1.5-, and 1.2-kb mRNAs are compatible with locations of two open coding regions in the DNA sequence (Herisse et al., Nucleic Acids Res. 9: [4023][4024][4025][4026][4027][4028][4029][4030][4031][4032][4033][4034][4035][4036][4037][4038][4039][4040][4041][4042] 1981). The relationship between the four polypeptides encoded by the 3.0-and 2.5-kb mRNAs and the two open coding regions is discussed. The production of multiple polypeptides from a heterogeneous mixture of mRNAs in the 0.8-kb size class is compatible with two large open coding regions in that part of the sequence. Thus, nearly all of the potential coding information in the leftward strand of E4 is expressed in translatable form during infection. Moreover, alternate splicing of the 0.8-kb mRNA size class can produce multiple polypeptides with common amino-terminal and different carboxy-terminal amino acid sequences, which may have the same function but different specificities.Early after adenovirus-2 infection, before the leftward direction from a promoter located at mp beginning of DNA replication, RNA is tran-...

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“…This binding along ssDNA is sequence‐independent, with one DBP monomer every 10–15 nucleotides . Although DBP has been best characterized in relation to its function in the replication of viral DNA, it has been implicated in a number of different processes including transcription, mRNA stability, and even capsid assembly . DBP is essential for viral DNA replication recapitulated in vitro .…”

Section: The Viral Replication Machinerymentioning

confidence: 99%

“…DBP is essential for viral DNA replication recapitulated in vitro . Temperature‐sensitive DBP mutant viruses fail to replicate in infected cells at nonpermissive temperatures . DBP may contribute to viral DNA replication in multiple ways.…”

Section: The Viral Replication Machinerymentioning

confidence: 99%

“…This suggests that expression of late genes from the MLP may require genome replication in cis . Interestingly, inhibition of viral genome replication also results in an increase in expression of viral early genes . It is therefore likely that a modification of the viral genome or an interaction with a trans‐acting factor that is dependent on replication in cis is required to switch from early to late transcriptional programs.…”

Section: Viral Genome Replication and Late Gene Expressionmentioning

confidence: 99%

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Viral and cellular interactions during adenovirus DNA replication

2019

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Possible role of the 72,000 dalton DNA-binding protein in regulation of adenovirus type 5 early gene expression

Cited by 118 publications

References 44 publications

Adenovirus DNA template for late transcription is not a replicative intermediate

Adenovirus DNA template for late transcription is not a replicative intermediate

Expression of adenovirus-2 early region 4: assignment of the early region 4 polypeptides to their respective mRNAs, using in vitro translation

Viral and cellular interactions during adenovirus DNA replication

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