Possible role of stromal-cell-derived factor-1/CXCR4 signaling on lymph node metastasis of oral squamous cell carcinoma

Uchida, Daisuke; Begum, Naseem; Almofti, Ammar; Nakashiro, Koh‐ichi; Kawamata, Hitoshi; Tateishi, Yohei; Hamakawa, Hiroyuki; Yoshida, Hideo; Sato, Mitsunobu

doi:10.1016/s0014-4827(03)00344-6

Cited by 152 publications

(160 citation statements)

References 46 publications

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“…B88 and HNt cells (10) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), 100 μg/ml streptomycin, and 100 U/ml penicillin in a humidified atmosphere of 95% air and 5% CO 2 at 37˚C.…”

Section: Methodsmentioning

confidence: 99%

“…After stimulation of the cells with or without SDF-1· under serum-starved conditions, the preparation of total RNA and reverse transcription were performed as described previously (10). E-cadherin, vimentin, snail, matrix metalloproteinase (MMP)-2, MMP-7, urokinase typeplasminogen activator (uPA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA were detected with Taqman ® gene expression assay (Applied Biosystems, Foster City, CA), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Western blotting was performed as described previously (10). The nitrocellulose membrane (Amersham Pharmacia Biotech, Uppsala, Sweden) was incubated with primary antibodies against vimentin, cytokeratin (Dako Corporation), ß-catenin (BD Biosciences), ß-actin (Sigma, St. Louis, MO) and phosphorylated-or total-ERK1/2 and Akt/PKB (Cell Signaling), followed by horseradish peroxidaseconjugated secondary antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…Among these chemokines and their receptors, the stromal cell-derived factor-1 (SDF-1), also referred to as the CXCL12)/CXCR4 system, has been demonstrated to be involved in lymph node or distant metastasis of several types of cancer (3)(4)(5)(6)(7)(8)(9). We have also reported the involvement of this system in lymph node metastasis associated with oral squamous cell carcinoma (SCC) (10,11). Moreover, we have recently found that the expression of CXCR4 in oral SCC at the primary site was significantly associated with lymph node metastasis, the mode of invasion, tumor recurrence, and patient prognosis (12).…”

Section: Introductionmentioning

confidence: 99%

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Epithelial-mesenchymal transition induced by the stromal cell-derived factor-1/CXCR4 system in oral squamous cell carcinoma cells

Onoue

Uchida²,

Begum³

et al. 2006

Int J Oncol

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Abstract. Epithelial-mesenchymal transition (EMT) refers to critical events occasionally observed during tumor progression, including invasion and metastasis, by which cancer cells acquire a fibroblast-like phenotype. Since the stromal cellderived factor-1 (SDF-1)/CXCR4 system can facilitate lymph node metastasis in oral squamous cell carcinoma (SCC), we have explored the possibility that this system might be involved in EMT. Oral SCC cells, B88 and HNt, which have functional CXCR4 and lymph node metastatic potential, were found to lose their epithelial cell morphology due to SDF-1. In this context, the downregulation of epithelial markers, cytokeratin, E-cadherin and ß-catenin, and the upregulation of mesenchymal marker, vimentin and snail were detected. Furthermore, upregulation of vimentin by treatment with SDF-1 was impaired by phosphatidylinositol 3 kinase (PI3K) inhibitor Wortmannin, but not by mitogenactivated protein kinase/extracellular signal-regulated kinase inhibitor U0126. In the type I collagen embedding culture, SDF-1-treated B88 cells formed protruding extensions, but the effect was impaired by treatment with Wortmannin. These results suggested that EMT induced by the SDF-1/ CXCR4 system might be involved in the lymph node metastasis of oral SCCs via activation of PI3K-Akt/PKB pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epithelial-mesenchymal transition induced by the stromal cell-derived factor-1/CXCR4 system in oral squamous cell carcinoma cells

Onoue

Uchida²,

Begum³

et al. 2006

Int J Oncol

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“…IL-20 induced more CXCR4 expression on the surface of IL-20-treated OC-3 cells than of the untreated control group. The involvement of CXCR4 signaling in OSCC was mediated by the activation of both the ERK and the AKT/PKB pathways (45). Therefore, IL-20-induced activation of ERK and AKT may cross-link to amplify the CXCR4 signaling for the chemotaxis of oral cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Anti–IL-20 Monoclonal Antibody Alleviates Inflammation in Oral Cancer and Suppresses Tumor Growth

Hsu

Wei

Shieh

et al. 2012

Molecular Cancer Research

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Perfluorocarbon Nanoemulsions for Combined Pulmonary siRNA Treatment of Lung Metastatic Osteosarcoma

Shen

Wang

et al. 2019

Advanced Therapeutics

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Lung metastatic osteosarcoma is a lethal disease afflicting children and adolescents, with a 5-year survival rate of less than 20%. The development of perfluorocarbon (PFC) nanoemulsions as systems for direct pulmonary delivery of combination drug/siRNA therapy is reported. Fluorinated polycation (F-PCX) with an inherent ability to inhibit C-X-C receptor type 4 (CXCR4) is synthesized and used as a surfactant in the preparation of F-PCX@PFC nanoemulsions. The nanoemulsions are loaded with siRNA against signal transducer and activator of transcription 3 (STAT3) for combined treatment of lung metastasis in osteosarcoma. Intratracheal instillation of the treatments significantly prolonged the survival of animals with established lung metastases. The treatment with F-PCX@PFC/siSTAT3 emulsion polyplexes decreased the number of myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs) in the tumors, while increasing CD8 + T cells infiltration andIFNγ secretion to overcome the immunosuppressive tumor microenvironment. The reported nanoemulsions represent a promising pulmonary treatment of lung metastatic osteosarcoma.

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Possible role of stromal-cell-derived factor-1/CXCR4 signaling on lymph node metastasis of oral squamous cell carcinoma

Cited by 152 publications

References 46 publications

Epithelial-mesenchymal transition induced by the stromal cell-derived factor-1/CXCR4 system in oral squamous cell carcinoma cells

Epithelial-mesenchymal transition induced by the stromal cell-derived factor-1/CXCR4 system in oral squamous cell carcinoma cells

Anti–IL-20 Monoclonal Antibody Alleviates Inflammation in Oral Cancer and Suppresses Tumor Growth

Perfluorocarbon Nanoemulsions for Combined Pulmonary siRNA Treatment of Lung Metastatic Osteosarcoma

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