Possible Role of Low Level Laser Therapy on Bone Turnover in Ovariectomized Rats

Saad, Azza; Yamany, Mamdouh El; Abbas, Osama A.; Yehia, Mona

doi:10.21608/jhiph.2009.20721

Cited by 5 publications

(7 citation statements)

References 18 publications

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“…2). These results are in agreement with a previous report that IR laser therapy improves bone synthesis in osteoporotic as well as in normal rats [29] . Interestingly, serum bone metabolism biomarkers, including 1,25 (OH) 2D3, BALP, and TRACP, were signi cantly increased, especially in ovariectomized rats (Fig.…”

Section: Discussionsupporting

confidence: 94%

Gut Microbiome-Mediated Changes in Bone Metabolism Upon Infrared Light Exposure in Rats

Yang

et al. 2020

Preprint

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Background Adequate sunlight exposure helps reduce bone loss and is important to bone health. Currently, about 90% of the world population spends a major portion of daily life under artificial lighting. Unlike sunlight, LED white light, the main source of artificial lighting, has no infrared radiation, which is known to be beneficial to human health. In artificial lighting environments, infrared supplementation may be used to simulate the effects of sunlight on bone metabolism. Results Here, we supplemented white LED exposure with infrared light in normal and ovariectomized rats for three consecutive months and examined bone turnover, bone mass, and bone density. We also analyzed the structure and function of gut microbiota in the rats. Infrared supplementation significantly reduced the abundance of Saccharibacteria and increased the abundance of Clostridiaceae 1 and Erysipelotrichaceae bacteria. Our results indicate that changes in the gut microbiome correlate well with bone mass and bone metabolism.Conclusions Our work demonstrates that infrared supplementation can have a positive effect on rat bone metabolism by affecting gut microbiota. Our findings will be important considerations in the future design of healthy lighting environments that prevent or possibly ameliorate osteoporosis.

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Section: Discussionsupporting

confidence: 94%

Gut Microbiome-Mediated Changes in Bone Metabolism Upon Infrared Light Exposure in Rats

Yang

et al. 2020

Preprint

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“…When LLLT is applied in ovariectomized rats, it leads to a significant increase in serum calcium, inorganic phosphorus, Table 3 Means and standard errors of tissue repair volume (V tr ), density of trabecular bone volume (Vv t ), total volume of newly formed trabecular bone (Vtot), and area of collagen fibers (A c ) in groups with 6 sessions: G4 (0 J/cm 2 ), G5 (20 J/cm 2 ), and G6 (30 J/cm 2 ) osteocalcin, and alkaline phosphatase and a significant decrease in deoxypyridinoline (U-DPD)/creatinine. It also increases calcium deposition, alkaline phosphatase in exposed bone, osteoblast numbers, and compact bone thickness and decreases the number of osteoclasts [45]. According to Kanenari and colleagues [46], a possible explanation for the fact that LLLT stimulates bone formation would be based on the MAP1A gene, which promotes microtubule assembly and its functional expression.…”

Section: Discussionmentioning

confidence: 99%

Low-level laser therapy improves bone formation: stereology findings for osteoporosis in rat model

et al. 2015

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Low-level laser therapy (LLLT) benefits bone metabolism, but its use needs to be standardized. We evaluated the effects of LLLT on bone defects in calvaria of ovariectomized rats. Stereology was used to calculate tissue repair volume (V tr ), density of trabecular bone volume (Vv t ), total volume of newly formed trabecular bone (Vtot), and the area occupied by collagen fibers (A C ). Fifty-four Wistar rats were submitted to bilateral ovariectomy, and bone defects were created in calvaria after 150 days. The animals were divided into nine groups (n = 6), and 24 h after defects, the treatment started with a 780-nm low-intensity GaAlAs laser: G1, G2, and G3 received 3 sessions of 0, 20, and 30 J/cm(2) respectively; G4, G5, and G6 received 6 sessions of 0, 20, and 30 J/cm(2), respectively; and G7, G8, and G9 received 12 sessions of 0, 20, and 30 J/cm(2), respectively. A normal distribution was found for all of the data. The test used to verify the normality was the Kolmogorov-Smirnov (KS, p > 0.05). The one-way ANOVA followed by Tukey's post hoc test was used for data processing. A difference of p < 0.05 was considered statistically significant. Groups G2 and G1 showed significance for V tr , Vv t , Vtot, and (A C ). Results were significant for (Vv t ) and (Vtot) between G3 and G1. There were no significant results between G5 and G4 as well as between G8 and G7. Groups G6 and G4 results showed statistical difference for V tr , Vv t , Vtot, and (A C ). Groups G9 and G7 showed significance for V tr , Vv t , Vtot, and (A C ). In conclusion, there was new bone formation in the groups that received 20 and 30 J/cm(2) when compared to control groups, but over time, the dose of 30 J/cm(2) showed better stereological parameters when compared to 20 J/cm(2).

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“…Therefore, it is important to investigate treatments that have osteogenic potential, the capability to stimulate bone formation, and prevent bone loss [22]. The osteogenic effects of PW LLLT on osteoblastic cell proliferation and bone metabolism are well known [23][24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, PW LLLT irradiation may play an important role in bone remodeling and should be a valuable option for the treatment of bone diseases such as OP. In vivo studies have shown that PW LLLT treatments led to faster orthodontic tooth movement [26], effectively enhanced bone formation, and decreased bone resorption in osteoporotic OVX-d rats [27].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the effects of LLLT on biomechanical properties of tibial diaphysis in two rat models of experimental osteoporosis by a three point bending test

et al. 2015

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Osteoporosis (OP) is a disease which causes bone loss and fractures, leading to severe pain and deformity. This study has aimed to assess the effects of pulsed wave low-level laser therapy (PW LLLT) on cortical bone in two experimental models of OP in rats. There were four ovariectomized (OVX-d) groups and four dexamethasone-treated groups. The healthy group were considered for baseline evaluations. At 14 weeks following ovariectomy, the OVX-d rats were further subdivided into the following: control rats with OP, OVX-d rats that received alendronate (1 mg/kg), OVX-d rats treated with LLLT, and OVX-d rats treated with alendronate and LLLT. The remaining rats received dexamethasone for 5 weeks and were divided into four groups: control, alendronate-treated rats (1 mg/kg), laser-treated rats, and laser-treated rats with concomitant administration of alendronate. The rats received alendronate for 30 days. LLLT (890 nm, 80 Hz, 0.972 J/cm(2)) was performed on the tibias three times per week for 8 weeks. After 8 weeks, tibias were extracted and submitted to a three-point bending test. PW LLLT did not increase the biomechanical parameters of osteoporotic bones compared to controls and healthy rats. PW LLLT associated with alendronate treatment significantly increased stress high load in OVX-d rats compared to the healthy group. PW LLLT at the current study parameters failed to cause beneficial biomechanical effects in the examined osteoporotic cortical bones. PW LLLT associated with alendronate treatment produced a more remarkable effect on bone strength in the ovariectomized induced OP rat model.

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Possible Role of Low Level Laser Therapy on Bone Turnover in Ovariectomized Rats

Cited by 5 publications

References 18 publications

Gut Microbiome-Mediated Changes in Bone Metabolism Upon Infrared Light Exposure in Rats

Gut Microbiome-Mediated Changes in Bone Metabolism Upon Infrared Light Exposure in Rats

Low-level laser therapy improves bone formation: stereology findings for osteoporosis in rat model

Evaluation of the effects of LLLT on biomechanical properties of tibial diaphysis in two rat models of experimental osteoporosis by a three point bending test

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